Small-Molecule Fluorescent Probes for Imaging and Detection of Reactive Oxygen, Nitrogen, and Sulfur Species in Biological Systems

Jiao, Xiaoyun; Li, Yong; Niu, Jinye; Xie, Xilei; Wang, Xu; Tang, Bo

doi:10.1021/acs.analchem.7b04234

Cited by 438 publications

(193 citation statements)

References 210 publications

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“…For read-out the use of fluorescence is typical in sensorbased measurements, where search probes are used for imaging and detection even in biological systems [12]. Frequently, modified nanoparticles are used e.g.…”

Section: Highlights Of Research In Analytical Chemistry -2018mentioning

confidence: 99%

European analytical column number 47

2019

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Section: Highlights Of Research In Analytical Chemistry -2018mentioning

confidence: 99%

European analytical column number 47

2019

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“…In the light of all this, it is obvious that these hetero-xanthene-based fluorophores may find applications in sensing and bioimaging, through their implementation as chemodosimeters or probes in sample matrices or in living biological systems which the pH level is sufficiently low to unveil their NIR emission [67]. A valuable example might be the development of fluorescent probes that target acidic organelles known to play specific and indispensable roles in cellular processes [68][69][70][71]. In this context, the lysosome, a key constituent of cellular digestive system containing hydrolytic enzymes that function only under acidic conditions, could be an attractive target.…”

Section: Stability and Photophysical Characterization Of Sulfone Analmentioning

confidence: 99%

Synthesis, stability and spectral behavior of fluorogenic sulfone-pyronin and sulfone-rosamine dyes

Dejouy¹,

Laly²,

Valverde³

et al. 2018

Dyes and Pigments

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The first synthesis of sulfone-pyronin and sulfone-rosamine dyes bearing optically tunable primary amino groups (acting as fluorogenic centers) is presented. Sulfone analogs of xanthene-based fluorophores have recently been highlighted as a new class of near-infrared (NIR) fluorescent dyes (Liu et al. ACS Appl Mater Interfaces 2016;8(35):22953-62), and the availability of fluorogenic derivatives is essential for the rapid construction of "turn-on" reactive probes for chemoselective bioimaging. However, these fluorescent anilines have been found to be unstable in aqueous physiological conditions due to the marked electrophilicity of their meso-position and hence its propensity to undergo nucleophilic attack by a water molecule or a hydroxyde anion. Further investigations have helped us to determine the acidic pH range at which the sulfone-rosamine 2 is fully-stable and a complete assessment of the photophysical properties could be performed. Its pro-fluorescent character and utility were then proved by the preparation and in vitro enzymatic activation of a penicillin G acylase (PGA) sensitive fluorogenic probe.

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“…[4][5][6][7] Although these methods use different techniques, they usually have some drawbacks. For example, traditional colorimetric and uorescent methods based on organic dyes have poor photostability and water solubility, and are expensive, 8 while, electrochemistry and isotopic labeling methods based on conjugated polyelectrolytes have complicated synthetic, purication, and modication procedures, which affect ALP activity. 9 Furthermore, in most cases, ALP is expressed at a low abundance level naturally in biological systems, so the detector signal for ALP levels has been affected by interference in human serum.…”

Section: Introductionmentioning

confidence: 99%

“…Comparison of our assay with reported methods for ALP activity detection RSC Adv.,2018,8,[36527][36528][36529][36530][36531][36532][36533] This journal is © The Royal Society of Chemistry 2018RSC Advances Paper Open Access Article. Published on 29 October 2018.…”

mentioning

confidence: 99%