Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes

Abstract

Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency of Agrobacterium-mediated transformation of a model legume, Lotus japonicus. Moreover, we explain the transformation protocol using chloroxynil, a phenolic compound that improves the efficiency.

A simple and efficient Agrobacterium-mediated in planta transformation protocol for horse gram (Macrotyloma uniflorum Lam. Verdc.)

“…There are several conventional breeding methods adopted to develop hybrid varieties with disease and pest resistance. Significant success was achieved in the transformation of forage and pasture legumes via Agrobacterium-mediated transformation, which is the most popular and efficient method of plant genetic engineering [16][17][18][19]. In this study, the "in planta" transformation method was studied, where Agrobacterium is used to infect the seedlings.…”