Small molecule-sensing strategy and techniques for understanding the functionality of green tea

Fujimura, Yoshinori

doi:10.1080/09168451.2014.996205

Cited by 3 publications

(3 citation statements)

References 119 publications

(132 reference statements)

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“…Among these are vimentin, Fyn, ZAP70, insulin-like growth factor 1 receptor, and glucose regulated protein 78 kDa [42,43,44,45,46]. However, the functional effect of green tea catechins on the target protein activity has been demonstrated only at much higher concentrations of EGCG than Kd values, probably because of the non-specific binding of EGCG to other proteins competing for the target [47]. In Table 1 are listed the principal EGCG molecular targets that were identified in cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Molecular Targets of Epigallocatechin—Gallate (EGCG): A Special Focus on Signal Transduction and Cancer

et al. 2018

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Green tea is a beverage that is widely consumed worldwide and is believed to exert effects on different diseases, including cancer. The major components of green tea are catechins, a family of polyphenols. Among them, epigallocatechin-gallate (EGCG) is the most abundant and biologically active. EGCG is widely studied for its anti-cancer properties. However, the cellular and molecular mechanisms explaining its action have not been completely understood, yet. EGCG is effective in vivo at micromolar concentrations, suggesting that its action is mediated by interaction with specific targets that are involved in the regulation of crucial steps of cell proliferation, survival, and metastatic spread. Recently, several proteins have been identified as EGCG direct interactors. Among them, the trans-membrane receptor 67LR has been identified as a high affinity EGCG receptor. 67LR is a master regulator of many pathways affecting cell proliferation or apoptosis, also regulating cancer stem cells (CSCs) activity. EGCG was also found to be interacting directly with Pin1, TGFR-II, and metalloproteinases (MMPs) (mainly MMP2 and MMP9), which respectively regulate EGCG-dependent inhibition of NF-kB, epithelial-mesenchimal transaction (EMT) and cellular invasion. EGCG interacts with DNA methyltransferases (DNMTs) and histone deacetylases (HDACs), which modulates epigenetic changes. The bulk of this novel knowledge provides information about the mechanisms of action of EGCG and may explain its onco-suppressive function. The identification of crucial signalling pathways that are related to cancer onset and progression whose master regulators interacts with EGCG may disclose intriguing pharmacological targets, and eventually lead to novel combined treatments in which EGCG acts synergistically with known drugs.

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Section: Introductionmentioning

confidence: 99%

Molecular Targets of Epigallocatechin—Gallate (EGCG): A Special Focus on Signal Transduction and Cancer

et al. 2018

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“…In most cases, evaluation of the attributes of crude samples in research on pharmaceuticals, nutraceuticals, and functional foods has focused on the absolute abundance of a single component as a biomarker 1 , while the importance of multicomponent information has mostly been neglected. In addition to the chemometric approach for applying such information to the evaluation and understanding of physiological activity, the development of a methodology for effectively selecting a meaningful combination of components in crude sample systems, on a sound theoretical basis, remains a challenge.…”

Section: Resultsmentioning

confidence: 99%

“…a low-molecular-weight bioactive compound); however, to accurately predict the real bioactivity of complicated multicomponent systems, the simultaneous evaluation of multiple coexisting factors is required 1 . Nevertheless, such an analytical approach remains to be established.…”

Section: Introductionmentioning

confidence: 99%

A Chemometrics-driven Strategy for the Bioactivity Evaluation of Complex Multicomponent Systems and the Effective Selection of Bioactivity-predictive Chemical Combinations

Fujimura

Kawano

Maeda-Murayama

et al. 2017

Sci Rep

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Although understanding their chemical composition is vital for accurately predicting the bioactivity of multicomponent drugs, nutraceuticals, and foods, no analytical approach exists to easily predict the bioactivity of multicomponent systems from complex behaviors of multiple coexisting factors. We herein represent a metabolic profiling (MP) strategy for evaluating bioactivity in systems containing various small molecules. Composition profiles of diverse bioactive herbal samples from 21 green tea extract (GTE) panels were obtained by a high-throughput, non-targeted analytical procedure. This employed the matrix-assisted laser desorption ionization–mass spectrometry (MALDI–MS) technique, using 1,5-diaminonaphthalene (1,5-DAN) as the optical matrix for detecting GTE-derived components. Multivariate statistical analyses revealed differences among the GTEs in their antioxidant activity, oxygen radical absorbance capacity (ORAC). A reliable bioactivity-prediction model was constructed to predict the ORAC of diverse GTEs from their compositional balance. This chemometric procedure allowed the evaluation of GTE bioactivity by multicomponent rather than single-component information. The bioactivity could be easily evaluated by calculating the summed abundance of a few selected components that contributed most to constructing the prediction model. 1,5-DAN-MALDI–MS-MP, using diverse bioactive sample panels, represents a promising strategy for screening bioactivity-predictive multicomponent factors and selecting effective bioactivity-predictive chemical combinations for crude multicomponent systems.

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Modified Green Tea Polyphenols, EGCG-S and LTP, Inhibit Endospore in Three <i>Bacillus</i> spp.

Ali¹,

Lee²,

Laskar³

et al. 2017

AiM

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Endospores have the ability to withstand extreme temperature, desiccation, ultraviolet radiation and chemicals which make them a threat to the food and healthcare industry. Green tea polyphenols (GTP), contain anti-microbial and anti-spore properties but not stable. In this study, two modified lipophilic green tea polyphenols, epigallocatechin-3-gallate-sterate (EGCG-S) and crude lipophilic green tea polyphenols (LTP), were used to compare their anti-spore effect with EGCG and crude GTP. Purified endospores from Bacillus cereus (B. cereus), B. megaterium and B. subtilis were treated with 1% or 5% of four tea polyphenols. Log reduction showed colony forming units (CFU) reduced significantly in all treated samples, ranging from 1.27 to 4.31 with no survivals (CFU = 0) in four samples (P < 0.05). Average percentage of inhibition for these polyphenols treatment ranged from 91.68% to 100%. The EGCG-S and LTP have equal or better anti-spore activities compared with EGCG and GTP.EGCG-S and LTP were further used to carry out time course study on B. cereus.The results indicated that 15 min of treatment of 1% and 5% LTP and EGCG-S are able to inhibit 98.7% to 100% of germination. Transmission and scanning electron microscopy studies showed that EGCG-S caused surface disruption and damaged spores structural integrity. EGCG-S and LTP are stable anti-spore agents may aid in preventing food and beverage spoilage caused by sporeforming bacteria as well as preventing contamination in the medical industry.

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Small molecule-sensing strategy and techniques for understanding the functionality of green tea

Abstract: To cite this article: Yoshinori Fujimura (2015) Small molecule-sensing strategy and techniques for understanding the functionality of green tea, Bioscience, Biotechnology, and Biochemistry, 79:5, 687-699,

Cited by 3 publications

References 119 publications

Molecular Targets of Epigallocatechin—Gallate (EGCG): A Special Focus on Signal Transduction and Cancer

Molecular Targets of Epigallocatechin—Gallate (EGCG): A Special Focus on Signal Transduction and Cancer

A Chemometrics-driven Strategy for the Bioactivity Evaluation of Complex Multicomponent Systems and the Effective Selection of Bioactivity-predictive Chemical Combinations

Modified Green Tea Polyphenols, EGCG-S and LTP, Inhibit Endospore in Three <i>Bacillus</i> spp.

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Small molecule-sensing strategy and techniques for understanding the functionality of green tea

Abstract: To cite this article: Yoshinori Fujimura (2015) Small molecule-sensing strategy and techniques for understanding the functionality of green tea, Bioscience, Biotechnology, and Biochemistry, 79:5, 687-699,

Cited by 3 publications

References 119 publications

Molecular Targets of Epigallocatechin—Gallate (EGCG): A Special Focus on Signal Transduction and Cancer

Molecular Targets of Epigallocatechin—Gallate (EGCG): A Special Focus on Signal Transduction and Cancer

A Chemometrics-driven Strategy for the Bioactivity Evaluation of Complex Multicomponent Systems and the Effective Selection of Bioactivity-predictive Chemical Combinations

Modified Green Tea Polyphenols, EGCG-S and LTP, Inhibit Endospore in Three &lt;i&gt;Bacillus&lt;/i&gt; spp.

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Modified Green Tea Polyphenols, EGCG-S and LTP, Inhibit Endospore in Three <i>Bacillus</i> spp.