Small Molecules for Dissecting Endomembrane Trafficking: A Cross-Systems View

Mishev, Kiril; Dejonghe, Wim; Russinova, Eugenia

doi:10.1016/j.chembiol.2013.03.009

Cited by 45 publications

(38 citation statements)

References 94 publications

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“…VTI12 also is used as a TGN marker (Geldner et al, 2009). Likewise, the treatment of brefeldin A (BFA) and wortmannin, endomembrane trafficking inhibitors (Hicks and Raikhel, 2010;Mishev et al, 2013), induced the formation of BFA compartments and ringlike structures of SCYL2B proteins (Supplemental Fig. S11).…”

Section: Discussionmentioning

confidence: 99%

SCYL2 Genes Are Involved in Clathrin-Mediated Vesicle Trafficking and Essential for Plant Growth

et al. 2017

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Protein transport between organelles is an essential process in all eukaryotic cells and is mediated by the regulation of processes such as vesicle formation, transport, docking, and fusion. In animals, SCY1-LIKE2 (SCYL2) binds to clathrin and has been shown to play roles in trans-Golgi network-mediated clathrin-coated vesicle trafficking. Here, we demonstrate that SCYL2A and SCYL2B, which are Arabidopsis (Arabidopsis thaliana) homologs of animal SCYL2, are vital for plant cell growth and root hair development. Studies of the SCYL2 isoforms using multiple single or double loss-of-function alleles show that SCYL2B is involved in root hair development and that SCYL2A and SCYL2B are essential for plant growth and development and act redundantly in those processes. Quantitative reverse transcription-polymerase chain reaction and a b-glucuronidase-aided promoter assay show that SCYL2A and SCYL2B are differentially expressed in various tissues. We also show that SCYL2 proteins localize to the Golgi, trans-Golgi network, and prevacuolar compartment and colocalize with Clathrin Heavy Chain1 (CHC1). Furthermore, bimolecular fluorescence complementation and coimmunoprecipitation data show that SCYL2B interacts with CHC1 and two Soluble NSF Attachment Protein Receptors (SNAREs): Vesicle Transport through t-SNARE Interaction11 (VTI11) and VTI12. Finally, we present evidence that the root hair tip localization of Cellulose Synthase-Like D3 is dependent on SCYL2B. These findings suggest the role of SCYL2 genes in plant cell developmental processes via clathrin-mediated vesicle membrane trafficking.

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Section: Discussionmentioning

confidence: 99%

SCYL2 Genes Are Involved in Clathrin-Mediated Vesicle Trafficking and Essential for Plant Growth

et al. 2017

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“…The endomembrane system is composed of specific membrane-delimited organelles, such as the endoplasmic reticulum, Golgi apparatus/TGN, plasma membrane, endosomes (early and late endosomes) and prevacuolar compartments/multivesicular bodies (Mishev et al, 2013;Shoji et al, 2014). Recently, accumulating evidence has shown that endomembrane trafficking and related organelles have a key role in maintaining physiological processes and pathogenicity in filamentous fungi (Shoji et al, 2014).…”

Section: Researchmentioning

confidence: 99%

Retrograde trafficking from the endosome to the trans‐Golgi network mediated by the retromer is required for fungal development and pathogenicity in Fusarium graminearum

Zheng

Zhao

et al. 2016

New Phytologist

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SummaryIn eukaryotes, the retromer is an endosome-localized complex involved in protein retrograde transport. However, the role of such intracellular trafficking events in pathogenic fungal development and pathogenicity remains unclear.The role of the retromer complex in Fusarium graminearum was investigated using cell biological and genetic methods.We observed the retromer core component FgVps35 (Vacuolar Protein Sorting 35) in the cytoplasm as fast-moving puncta. FgVps35-GFP co-localized with both early and late endosomes, and associated with the trans-Golgi network (TGN), suggesting that FgVps35 functions at the donor endosome membrane to mediate TGN trafficking. Disruption of microtubules with nocodazole significantly restricted the transportation of FgVps35-GFP and resulted in severe germination and growth defects. Mutation of FgVPS35 not only mimicked growth defects induced by pharmacological treatment, but also affected conidiation, ascospore formation and pathogenicity. Using yeast two-hybrid assays, we determined the interactions among FgVps35, FgVps26, FgVps29, FgVps17 and FgVps5 which are analogous to the yeast retromer complex components. Deletion of any one of these genes resulted in similar phenotypic defects to those of the DFgvps35 mutant and disrupted the stability of the complex.Overall, our results provide the first clear evidence of linkage between the retrograde transport mediated by the retromer complex and virulence in F. graminearum.

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“…Over the recent years though, plant biology has witnessed an increasing interest in chemical biology approaches for processes such as endomembrane trafficking, hormonal signaling and primary and secondary metabolism (Hicks and Raikhel, 2012; Mishev et al, 2013; Tresch, 2013; Ma and Robert, 2014). Depending on which aspect of plant biology the small molecule of choice is affecting, and what the intended use will be, knowledge on the interactome of the small molecule might be essential.…”

Section: Future Perspectivesmentioning

confidence: 99%

Target identification strategies in plant chemical biology

Dejonghe

Russinova

2014

Front. Plant Sci.

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The current needs to understand gene function in plant biology increasingly require more dynamic and conditional approaches opposed to classic genetic strategies. Gene redundancy and lethality can substantially complicate research, which might be solved by applying a chemical genetics approach. Now understood as the study of small molecules and their effect on biological systems with subsequent target identification, chemical genetics is a fast developing field with a strong history in pharmaceutical research and drug discovery. In plant biology however, chemical genetics is still largely in the starting blocks, with most studies relying on forward genetics and phenotypic analysis for target identification, whereas studies including direct target identification are limited. Here, we provide an overview of recent advances in chemical genetics in plant biology with a focus on target identification. Furthermore, we discuss different strategies for direct target identification and the possibilities and challenges for plant biology.

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Small Molecules for Dissecting Endomembrane Trafficking: A Cross-Systems View

Cited by 45 publications

References 94 publications

SCYL2 Genes Are Involved in Clathrin-Mediated Vesicle Trafficking and Essential for Plant Growth

SCYL2 Genes Are Involved in Clathrin-Mediated Vesicle Trafficking and Essential for Plant Growth

Retrograde trafficking from the endosome to the trans‐Golgi network mediated by the retromer is required for fungal development and pathogenicity in Fusarium graminearum

Target identification strategies in plant chemical biology

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