Small Nucleolar RNA Host Gene 1 (SNHG1) and Chromosome 2 Open Reading Frame 48 (C2orf48) as Potential Prognostic Signatures for Liver Cancer by Constructing Regulatory Networks

Abstract

Background: Liver cancer is a common malignant tumor with poor prognosis. The present study sought to identify potential signatures that can predict the prognosis of patients with liver cancer. Material/Methods: The RNA sequencing (RNA-seq) and clinical information of liver cancer patients were obtained from the Cancer Genome Atlas (TCGA) database. Differentially expressed long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) were identified between liver cancer and adjacent normal tissues. After predicting lncRNA-miRNA and miRNA-mRNA pairs using online databases, the competing endogenous RNA (ceRNA) networks were constructed. Furthermore, the prognostic value of these differentially expressed genes was evaluated using univariate and multivariate Cox regression analyses. Results: After constructing the ceRNA network, 2 lncRNAs small nucleolar RNA host gene 1 (SNHG1) and chromosome 2 open reading frame 48 (C2orf48) with the most nodes were identified. Correlation analysis revealed that SNHG1 was correlated with miR-195 and C2orf48 was correlated with miR-195 and miR-93. High expression of SNHG1, C2orf48, and miR-93 can contribute to poorer clinical outcomes compared to low expression. Furthermore, low miR-195 expression was correlated with shorter survival time than was high expression. SNHG1 and C2orf48 were closely associated with histology grade. Univariate and multivariate Cox regression analyses confirmed that SNHG1 and C2orf48 are risk factors for liver cancer. Conclusions: Our findings revealed that SNHG1 and C2orf48 possess potential prognostic value and should be considered as possible biomarkers for predicting clinical outcomes for patients with liver cancer.

The Association between Long Noncoding RNA over Expression and Poor Prognosis of Liver Cancer: A Meta-Analysis

“…( 4) e cutoff value of lncRNA expression level was described. (5) Every study performed the association between expression of lncRNA and OS, and the hazard ratio (HR) value and its corresponding 95% confidence interval (95% CI) for OS was either obtained directly from actual study or indirectly it will be calculated by Engauge Digitizer 4.1 software from survival curve. (6) e hazard ratio (HR) value and its corresponding 95% confidence interval (95% CI) for the OS, RFS, and DFS were either directly acquired from the original text or will be indirectly estimated by Engauge Digitizer 4.1 software from the survival curve [13]; (4) risk of bias assessed the key factors.…”

“…lncRNA is a kind of endogenic RNA 200 or more nucleotides long, which is extensively present in Homo sapiens. It has no protein-coding function and lacks a specific open reading frame (ORF) [5], which was considered to be a nonsignificant byproduct of transcription in the early stage [6][7][8]. Over the years, due to the advancement of highresolution chips and high-throughput sequencing technologies, the research on transcriptome regulation has been increasingly deepened, and it is found that lncRNA not only plays a significant role in transcriptional interference but also in the process of some histological development, tumorigenesis, and tumor metastasis [9][10][11].…”

Knockdown of lncRNA LINC01234 Suppresses The Tumorigenesis of Liver Cancer via Sponging miR-513a-5p

“…Introduction Liver cancer is a common malignant tumor, with a 15-17% 5-year survival rate (1,2). The prognosis of patients with liver cancer is poor due to the high frequency of postoperative recurrence and metastasis (3).…”

Knockdown of lncRNA LINC01234 suppresses the tumorigenesis of liver cancer via sponging miR-513a-5p

“…Liver cancer is a common malignant tumor, with a 15-17% 5-year survival rate (1,2). The prognosis of patients with liver cancer is poor due to the high frequency of postoperative recurrence and metastasis (3).…”