Small RNA in tolerating various biotic stresses

Dutta, Summi; Afreen, Uzma; Kumar, Manish; Mukhopadhyay, Kunal

doi:10.1016/b978-0-12-817112-7.00008-0

Cited by 2 publications

(1 citation statement)

References 109 publications

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“…miRNAs play crucial roles in plant developmental processes such as juvenile-to-adult transition, shoot apical meristem development, leaf morphogenesis, oral organ formation, and owering time determination (Dong, et al 2022). sRNAs are involved in plant response to abiotic stresses (Dutta, et al 2020;Singh, et al 2019). miRNAs play a crucial role in regulating genes involved in plant responses to drought stress.…”

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confidence: 99%

ABA-responsive small RNAs contribute to drought stress memory by regulating antioxidant activity and lignification

Khoshniat,

Rafudeen,

Seifi

2023

Preprint

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Previously we showed that a single exogenous application of Abscisic acid (ABA) on Arabidopsis in early developmental stages results in enhanced plant biomass and drought tolerance in the adult plants. Here we tested the hypothesis that small RNAs (sRNAs), including micro RNAs (miRNAs) and small interfering RNAs (siRNAs), contribute to the observed ABA effects. We sequenced sRNA populations in Arabidopsis plants that were sprayed with ABA or water at the cotyledonary stage at two time-points, 4 hours and 14 days after spraying. Analyses of the sRNAs revealed that at 4 hours, four miRNA families, miR408, miR398, miR397, and miR393, were significantly down-regulated by the ABA application. These miRNAs are all copper-induced miRNAs, which are known to be involved in the regulation of antioxidant enzymes and lignification metabolic pathways. After 14 days, miR395 and miR169 families were up-regulated, while miR399 and miR827 families were down-regulated in ABA-treated plants. These miRNAs are mostly characterized as regulators of macronutrient uptake. We identified 77 and 30 differentially expressed siRNA loci in the first and second time-points respectively. Interestingly, a majority of these ABA-responsive siRNAs were found to be mainly associated with the non-coding regions of the genome, as well as with genes related to photosynthesis, particularly those encoding light-harvesting complex proteins. In summary, our data suggest that ABA-responsive miRNAs contribute to the positive effects of ABA by increasing plant antioxidant capacity and lignification in the short-term while increasing nutrient uptake in the long term.

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Section: )mentioning

confidence: 99%

ABA-responsive small RNAs contribute to drought stress memory by regulating antioxidant activity and lignification

Khoshniat,

Rafudeen,

Seifi

2023

Preprint

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Comprehensive analysis of hairpin small RNAs involved in resistance and pathogenesis during wheat–Puccinia triticina interactions

Afreen,

Pandey,

Jha

et al. 2024

Physiologia Plantarum

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Wheat leaf rust, caused by the fungus Puccinia triticina (Pt), severely affects the grain quality and quantity of bread wheat (Triticum aestivum L.). Hairpin small(s)RNAs, like micro(mi)RNAs and their variants [including isomiRNAs (isomiRs) and microRNA‐like RNAs (milRNAs)], along with their corresponding target genes, bestow leaf rust disease resistance, development and progression from both interacting species. However, the regulatory networks remain inadequately understood. Thirteen differentially expressed novel miRNAs, including two isomiRs and three milRNAs were discerned from induced reads of wheat sRNA libraries, and a further 5,393 and 1,275 candidate target genes were predicted in wheat and Pt, respectively. Functional annotation divulged that wheat‐originated miRNAs/isomiRs were involved in resistance, while Pt‐derived milRNAs imparted pathogenesis. The identified milRNAs‐ Tae‐Pt‐milR5, Tae‐Pt‐milR12, and Tae‐Pt‐milR14b and their cleavage sites on Pt target gene MEP5 were confirmed through degradome library screening, suggesting cross‐kingdom translocation of Pt virulent genes in wheat host. Co‐expression analysis of miRNAs/isomiRs‐target genes provided insights into combating leaf rust disease, while co‐expression analysis of milRNAs‐target gene pairs reflected the extent of pathogenicity exerted by Pt with varied expression levels at the analyzed time points. The analysis pinpointed leaf rust‐responsive candidate hairpin sRNAs‐ Tae‐miR8, Tae‐Pt‐miR12, Tae‐Pt‐miR14a, and Tae‐Pt‐miR14b in wheat and Tae‐Pt‐milR12 in Pt. This study provides new insights into the hairpin sRNAs involved in the resistance and pathogenesis of wheat and Pt, respectively. Furthermore, crucial hairpin sRNAs and their promising targets for future biotechnological interventions to augment stress resilience have been identified.

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Small RNA in tolerating various biotic stresses

Cited by 2 publications

References 109 publications

ABA-responsive small RNAs contribute to drought stress memory by regulating antioxidant activity and lignification

ABA-responsive small RNAs contribute to drought stress memory by regulating antioxidant activity and lignification

Comprehensive analysis of hairpin small RNAs involved in resistance and pathogenesis during wheat–Puccinia triticina interactions

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