Small RNA-Sequencing Library Preparation for the Halophilic Archaeon Haloferax volcanii

The use of RNA-seq for the study of physiological adaptations of halophiles in extreme environments for astrobiological data interpretation

“…Our hypothesis is bolstered by these reports on using RNA-seq in halophiles research as described below: Chromohalobacter salexigens (Salvador et al, 2018), Cenococcum geophilum (Li J. et al, 2022), Halobacterium salinarum NRC-1 (Pastor et al, 2022;Lorenzetti et al, 2023;Ten-Caten et al (2018), Haloferax mediterranei ATCC33500 (Artieri et al, 2017;Payá et al, 2020;Pastor et al, 2022), Haloarcula hispainca DF60 (Pastor et al, 2022), Halolamina sp. YKT1 (Kurt-Kızıldoğan et al, 2017), Halomonas bluephagenesis TD01 (Xu et al, 2022), Halomonas socia NY-011 (Peng et al, 2020), Haloferax volcanii (Ammar et al, 2012;Babski et al, 2016;Artieri et al, 2017;Gebetsberger et al, 2017;Laass et al, 2019;Gelsinger and DiRuggiero, 2022;Pastor et al, 2022;Pastor et al, 2023), Haloferax alexandrinus DSM 27206 (Buda et al, 2023), Dunaliella bardawil (Liang et al, 2020), Tetraselmis sp. (D'Adamo et al, 2014), Dunaliella salina (Gao et al, 10.3389/fspas.2024.1342694 2023), Euhalothece sp.…”