Smaller Copper Oxide Nanoparticles have More Biological Effects Versus Breast Cancer and Nosocomial Infections Bacteria

Abstract

Background and Objectives: Despite promising successes in developing new drugs and pharmaceutical biotechnology, infectious diseases and cancer are still the principal causes of mortality and morbidity globally. Therefore, finding effective ways to deal with these pathogens and cancers is critical. Metal nanoparticles are one of the new strategies to combat bacteria and cancers. Methods: We examined the antimicrobial activity of 30 and 60 nm copper oxide nanoparticles (CuO-NPs) against Acinetobacter baumannii and Staphylococcus epidermidis bacteria responsible for nosocomial infections in standard and clinical strains and anti-cancer activity against 4T1 cell line as malignancy breast cancer cells. Synthesis of CuO-NPs was performed by a one-step reduction method and confirmed by DLS and TEM microscopy at 30 and 60 nm sizes. The antibacterial and anti-cancer activities of the nanoparticles were then investigated against the aforementioned bacteria and breast cancer. Results: Using disk, well, MIC, MBC methods, and viability/bacterial growth assay, 30 nm CuO NPs were found to have more antibacterial activity on standard and clinical strains than 60 nm CuO NPs. On the other hand, using MTT, apoptosis, and gene expression method, 30 nm nanoparticles were found to have more anti-cancer potential than 60 nm CuO NPs. Conclusions: Our findings implicate CuO-NPs to possess antimicrobial and anti-cancer effects and more significant potential in smaller sizes, suggesting their pharmaceutical and biomedical capacity.

Biogenic copper oxide nanoparticles from Bacillus coagulans induced reactive oxygen species generation and apoptotic and anti-metastatic activities in breast cancer cells

“…The present study’s findings are in line with those of other studies; nevertheless, the elevated concentration of CuONPs caused a significant reduction in VEGF expression and elevated apoptosis in 4T1 cancer cells 90 . In a previous investigation conducted by Luo et al 91 , a dose- and time-dependent reduction was observed in the levels of cyclin A and cyclin B1 in HaCaT cells treated with CuONPs.…”

Antibiotics Combinations and Chitosan Nanoparticles for Combating Multidrug Resistance Acinetobacter baumannii

“…The obtained results were in accordance with many recent studies conducted in 2021 on the antimicrobial activities of different nanoparticles either alone or in combination with other antimicrobials have proved activity against various pathogens. These included, Casein-silver NPs combined with TGC against A. baumannii , 72 antibiofilm and anti-virulence potential of silver NPs against MDR A. baumannii , 73 Cu:Ag bimetallic NPs for antibiotic-resistant bacteria, 74 Lignin-Capped silver NPs, 75 Smaller Copper Oxide Nanoparticles against MDR Bacteria, 76 colistin-integrated chitosan nanoparticles. 77 Our results revealed that CNPs when combined with MEM significantly increased the susceptibilities of the MDR A. baumannii isolates by 100% as compared to MEM alone.…”

Multiprocessing Substrates Enhanced Ta2O5/NiCo2O4 Spinel Nanocomposites for Effective Electro-/Photocatalytic and Toxicological Effects via the Caenorhabditis elegans Model

“…As a result, the small particle size of the RTNCO composite improved the antibacterial performance against bacteria, and the remaining three composites, which have a larger particle size, demonstrated a weaker antibacterial effect. 47 The reactive oxygen species (ROS) test was also carried out to determine the oxidative stress on the bacterial cell membrane caused by free radicals during the testing. Excessive levels of ROS produce higher oxidative stress on the cell wall, which damages protein linkages, DNA cleavage, nucleic acids, and the respiratory chain.…”