“SMART” digital nucleic acid amplification technologies for lung cancer monitoring from early to advanced stages

Rapid Detection of Uropathogens Using an Integrated Multiplex Digital Nucleic Acid Detection Assay Powered by a Digital-to-Droplet Microfluidic Device

“…3,4 Digital detection provides precise measurements and allows for accurate quantification of small amounts of targets. 5,6 Droplet microfluidics is advantageous for digital detection assays as it offers the capability of generating a large number of droplets for highly efficient sample compartmentalization. 7,8 Such assays are suited for various targets including nucleic acid, protein, and cell, making it extensively employed in biomedical research and clinical diagnostics.…”

“…A digital detection assay is a quantitative analysis method that relies on large-scale dispersion systems and employs the Poisson distribution algorithm. , This approach estimates the concentration of the detection target by evaluating the proportion of statistically positive units, leading to significant enhancements in detection sensitivity and quantitation accuracy. , Digital detection provides precise measurements and allows for accurate quantification of small amounts of targets. , Droplet microfluidics is advantageous for digital detection assays as it offers the capability of generating a large number of droplets for highly efficient sample compartmentalization. , Such assays are suited for various targets including nucleic acid, protein, and cell, making it extensively employed in biomedical research and clinical diagnostics. − …”