Smart Immobilization of oligopeptides through electrochemical deposition onto surface

Functionalisation of solid material surface with functional protein molecules for bioelectronic properties

“…16 The EC tag method is based on the electrochemical deposition of a coordinated metal ion in peptidic ligand. 17 The peptidic ligand (at least three amino acids in length) can be introduced at any site on the enzyme molecule by genetic techniques and can be tagged onto synthesised organic compounds (e.g. oligopeptides) or inorganic materials (e.g.…”

Electrochemical preparation of junction between a molecule and solid surface through a metal coordinative peptidic tag

“…The ECtag-PEG molecule and ECtag-PEG-FITC were prepared as detailed in a previous report [13]. A purified functional ECtag-PEG-FITC molecule was obtained as a yellow powder after lyophilization.…”

“…We employed this finding to develop a macromolecular immobilization method (including the immobilization of a nano-particle), which we call the "ECtag method." [13]. However, the junction (the interaction between ECtag ligand and deposited Cu) has not yet been clarified.…”

Suppressive Method for Unspecific Adsorption on a Semiconductor Electrode in the Specific Molecular Immobilization Process Using EC tag Method

“…26 The EC tag tagged molecule was prepared as detailed in a previous report. 19 A purified functional EC tag-PEGCy3 molecule was obtained as a red powder after lyophilization.…”

“…The authors have developed a smart immobilization technique, the "EC tag method", 19,20 that is a novel way to immobilize molecules through electrochemical reduction. EC tag is a peptidic ligand (such as hexahistidine) and forms a complex with divalent metal ions 21,22 as such as a copper (II).…”