Smart Shockwave Responsive Titania-Based Nanoparticles for Cancer Treatment

Vighetto, Veronica; Racca, Luisa; Canta, Marta; Matos, Joana C.; Dumontel, Bianca; Gonçalves, M. Clara; Cauda, Valentina

doi:10.3390/pharmaceutics13091423

Cited by 15 publications

(12 citation statements)

References 33 publications

(49 reference statements)

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“…In this context, Chen et al employed the adsorption of functional proteins (bone morphogenetic protein 2 and sclerostin antibody) to modify TiO 2 nanotube arrays to repair bone fractures [ 35 ]. The PC alters biodistribution, biological identity and stability, toxicity, and ultimately the fate of TiO 2 nps [ 36 ]. Thus, there is a need to meticulously characterize the nanomaterial properties, emphasizing particle size, crystal structure, and specific surface area, for a reliable prediction of the toxicological behavior of TiO 2 nanomaterials.…”

Section: Reviewmentioning

confidence: 99%

Engineered titania nanomaterials in advanced clinical applications

Sahare¹,

Alvarez²,

Yanez³

et al. 2022

Beilstein J. Nanotechnol.

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Significant advancement in the field of nanotechnology has raised the possibility of applying potent engineered biocompatible nanomaterials within biological systems for theranostic purposes. Titanium dioxide (titanium(IV) oxide/titania/TiO2) has garnered considerable attention as one of the most extensively studied metal oxides in clinical applications. Owing to the unique properties of titania, such as photocatalytic activity, excellent biocompatibility, corrosion resistance, and low toxicity, titania nanomaterials have revolutionized therapeutic approaches. Additionally, titania provides an exceptional choice for developing innovative medical devices and the integration of functional moieties that can modulate the biological responses. Thus, the current review aims to present a comprehensive and up-to-date overview of TiO2-based nanotherapeutics and the corresponding future challenges.

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Section: Reviewmentioning

confidence: 99%

Engineered titania nanomaterials in advanced clinical applications

Sahare¹,

Alvarez²,

Yanez³

et al. 2022

Beilstein J. Nanotechnol.

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“…cervical adenocarcinoma KB cell line (ATCC CCL17TM), which grows in adhesion as a 2D monolayer. KB cells were grown in Eagle's Minimum Essential Medium (EMEM, Sigma) supplemented with 10% heat inactivated fetal bovine serum (FBS, Sigma), 100 units/mL penicillin and 100 µg/mL streptomycin (Sigma), and maintained as reported for Daudi cell line Vighetto et al 2021).…”

Section: Cells Line and Treatmentsmentioning

confidence: 99%

A comparative analysis of low intensity ultrasound effects on living cells: from simulation to experiments

Campanini¹,

Vighetto²,

Racca³

et al. 2022

Biomed Microdevices

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Ultrasounds are already broadly exploited in clinical diagnostics and are now becoming a powerful and not harmful tool in antitumoral therapies, as they are able to produce damages towards cancer cells, thank to inertial cavitation and temperature increase. The use of US alone or combined to molecular compounds, microbubbles or solid-state nanoparticles is the focus of current research and clinical trials, like thermoablation, drug sonoporation or sonodynamic therapies. In the present work, we discuss on the non-thermal effects of ultrasound and the conditions which enable oxygen radical production and which role they can have in provoking the death of different cancer cell lines. In this perspective, we set a mathematical model to predict the pressure spatial distribution in a defined water sample volume and thus obtain a map of acoustic pressures and acoustic intensities of the applied ultrasound at different input powers. We then validate and verify these numerical results with direct acoustic measurements and by detecting the production of reactive oxygen species (ROS) by means of sonochemiluminescence (SCL) and electron paramagnetic resonance (EPR) spectroscopy, applied to the same water sample volume and using the same US input parameters adopted in the simulation. Finally, the various US conditions are applied to two different set of cancer cell lines, a cervical adenocarcinoma and a hematological cancer, Burkitt’s lymphoma. We hypothesize how the ROS generation can influence the recorded cell death. In a second set of experiments, the role of semiconductor metal oxide nanocrystals, i.e. zinc oxide, is also evaluated by adding them to the water and biological systems. In particular, the role of ZnO in enhancing the ROS production is verified. Furthermore, the interplay among US and ZnO nanocrystals is evaluated in provoking cancer cell death at specific conditions. This study demonstrates a useful correlation between numerical simulation and experimental acoustic validation as well as with ROS measurement at both qualitative and quantitative levels during US irradiation of simple water solution. It further tries to translate the obtained results to justify one of the possible mechanisms responsible of cancer cell death. It thus aims to pave the way for the use of US in cancer therapy and a better understanding on the non-thermal effect that a specific set of US parameters can have on cancer cells cultured in vitro. Graphical abstract

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“…To overcome the limitations of conventional drugs, such as limited solubility (hydrophobic and water-insoluble), failure to selectively target the disease site, adverse effects, and undesirable pharmacodynamics, many studies have been conducted in recent years toward developing more effective drug delivery systems, highlighting the importance of a high level of biocompatibility, loading capacity, and unique structural properties [7,8]. Titanium (Ti) and its alloys, which are currently used in clinical surgeries, are promising candidates for this purpose, as they are extremely durable, nonreactive to human tissues (e.g., dental and bone), and incompatible with living tissue [9][10][11][12]. Despite these, they can neither form strong bonds with bone during the early stages of osseointegration, nor carry/deliver bioactive agents and provide sustained release.…”

Section: Introductionmentioning

confidence: 99%

“…Titanium (Ti) and its alloys, which are currently used in clinical surgeries, are promising candidates for this purpose, as they are extremely durable, nonreactive to human tissues (e.g., dental and bone), and incompatible with living tissue [ 9 , 10 , 11 , 12 ]. Despite these, they can neither form strong bonds with bone during the early stages of osseointegration, nor carry/deliver bioactive agents and provide sustained release.…”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin-Loaded Titanium Nanotubes Coated with Chitosan: A Promising Formulation with Sustained Release and Enhanced Antibacterial Properties

et al. 2022

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Due to their high entrapment efficiency, anodized titanium nanotubes (TiO2-NTs) are considered effective reservoirs for loading/releasing strong antibiotics whose systemic administration is associated with diverse and severe side-effects. In this study, TiO2-NTs were synthesized by anodic oxidation of titanium foils, and the effects of electrolyte percentage and viscosity on their dimensions were evaluated. It was found that as the water content increased from 15 to 30%, the wall thickness, length, and inner diameter of the NTs increase from 5.9 to 15.8 nm, 1.56 to 3.21 µm, and 59 to 84 nm, respectively. Ciprofloxacin, a highly potent antibiotic, was loaded into TiO2-NTs with a high encapsulation efficiency of 93%, followed by coating with different chitosan layers to achieve a sustained release profile. The prepared formulations were characterized by various techniques, such as scanning electron microscopy, differential scanning calorimetry, and contact measurement. In vitro release studies showed that the higher the chitosan layer count, the more sustained the release. Evaluation of antimicrobial activity of the formulation against two endodontic species from Peptostreptococcus and Fusobacterium revealed minimum inhibitory concentrations (MICs) of 1 µg/mL for the former and the latter. To summarize, this study demonstrated that TiO2-NTs are promising reservoirs for drug loading, and that the chitosan coating provides not only a sustained release profile, but also a synergistic antibacterial effect.

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Smart Shockwave Responsive Titania-Based Nanoparticles for Cancer Treatment

Cited by 15 publications

References 33 publications

Engineered titania nanomaterials in advanced clinical applications

Engineered titania nanomaterials in advanced clinical applications

A comparative analysis of low intensity ultrasound effects on living cells: from simulation to experiments

Ciprofloxacin-Loaded Titanium Nanotubes Coated with Chitosan: A Promising Formulation with Sustained Release and Enhanced Antibacterial Properties

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