Smart SU-8 pillars implemented in a microfluidic bioreactor for continuous measurement of glucose

Talaei, Sara; Frey, Olivier; Psoma, Sotiria D.; Wal, P. D. van der; Rooij, N. F. de

doi:10.1016/j.proeng.2010.09.143

Cited by 6 publications

(8 citation statements)

References 2 publications

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“…Different principles of glucose sensors based on electrochemical and optical detection were recently proposed in the literature for application in BRs [ 247 , 248 , 249 , 250 , 251 , 252 , 253 , 254 , 255 , 256 , 257 ]. The innovative combination of droplet microfluidics and optical detection methods was proposed by Adams and coworkers for rapid measurements of glucose levels in the µBR systems [ 247 ].…”

Section: Sensorsmentioning

confidence: 99%

“…The methodology for surface modification of electrodes with enzymes typically involves the reaction with glutaraldehyde to crosslink the enzymes with proteins (such as bovine serum albumin) and/or polymers containing free amine groups. In addition, novel methodologies are being demonstrated, such as immobilizing enzymes (glucose oxidase) on SU-8 surfaces [ 249 ]. The attachment between SU-8 and the enzyme is enabled by binding unreacted epoxy groups of SU-8 to the free NH 2 group of glucose oxidase.…”

Section: Sensorsmentioning

confidence: 99%

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Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Djisalov

Knežić

Podunavac

et al. 2021

Biology

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Meat cultivation via cellular agriculture holds great promise as a method for future food production. In theory, it is an ideal way of meat production, humane to the animals and sustainable for the environment, while keeping the same taste and nutritional values as traditional meat and having additional benefits such as controlled fat content and absence of antibiotics and hormones used in the traditional meat industry. However, in practice, there is still a number of challenges, such as those associated with the upscale of cultured meat (CM). CM food safety monitoring is a necessary factor when envisioning both the regulatory compliance and consumer acceptance. To achieve this, a multidisciplinary approach is necessary. This includes extensive development of the sensitive and specific analytical devices i.e., sensors to enable reliable food safety monitoring throughout the whole future food supply chain. In addition, advanced monitoring options can help in the further optimization of the meat cultivation which may reduce the currently still high costs of production. This review presents an overview of the sensor monitoring options for the most relevant parameters of importance for meat cultivation. Examples of the various types of sensors that can potentially be used in CM production are provided and the options for their integration into bioreactors, as well as suggestions on further improvements and more advanced integration approaches. In favor of the multidisciplinary approach, we also include an overview of the bioreactor types, scaffolding options as well as imaging techniques relevant for CM research. Furthermore, we briefly present the current status of the CM research and related regulation, societal aspects and challenges to its upscaling and commercialization.

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Section: Sensorsmentioning

confidence: 99%

Section: Sensorsmentioning

confidence: 99%

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Djisalov

Knežić

Podunavac

et al. 2021

Biology

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“…The diffusion efficiency was influenced with diffusion resistance of the sensing film, and the diffusion resistance of sensing film decreased with increasing the flow rate. Furthermore, the high flow rate would make the enzyme membrane to fall off and caused the lower sensitivity and linearity [28], [35], [36].…”

Section: E Dynamical Measurement Of the Arrayed Glucose Biosensormentioning

confidence: 99%

Fabrication and Investigation of Arrayed Glucose Biosensor Based on Microfluidic Framework

Chou

Cheng

et al. 2013

IEEE Sensors J.

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In this paper, an arrayed glucose biosensor is integrated to the poly-dimethylsiloxane microchannel as the microfluidic device for measuring the characteristics of the arrayed glucose biosensor at dynamical conditions. The radio frequency sputtering system and screen printed technology are used to fabricate the arrayed glucose biosensor. Furthermore, the computer numerical control technique is used to fabricate the microchannel mold. The polypyrrole differential reference electrode has good stable characteristics and provides a stable potential for the arrayed pH sensor. At static conditions, the average sensitivity and linearity of the arrayed pH sensor are 57.85 mV/pH and 0.977, respectively. And at dynamical conditions with 20 mL/min flow rate, the average sensitivity and linearity of the arrayed pH sensor are 66.73 mV/pH and 0.993, respectively. The arrayed pH sensor is dropped glucose sensing membranes as an arrayed glucose biosensor. The arrayed glucose biosensor is measured in different glucose concentrations at static conditions, and the average sensitivity and linearity of the arrayed glucose biosensor are 11.84 mV(100 mg/dl) −1 and 0.995, respectively. At dynamical conditions with 25 mL/min flow rate, the average sensitivity and linearity of the arrayed glucose biosensor are 30.41 mV(100 mg/dl) −1 and 0.975, respectively.

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“…Previously, we introduced a simple technique for immobilization of enzymes on SU-8 surface without the need for pretreatment of the surface (Talaei et al 2010(Talaei et al , 2011(Talaei et al , 2012. A similar method was reported for immobilization of enzymes on other photoresists as well as SU-8 (Thomas et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Enzyme SU-8 microreactors: simple tools for cell-culture monitoring

Talaei

Wal

Ahmed

et al. 2015

Microfluid Nanofluid

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Smart SU-8 pillars implemented in a microfluidic bioreactor for continuous measurement of glucose

Cited by 6 publications

References 2 publications

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Cultivating Multidisciplinarity: Manufacturing and Sensing Challenges in Cultured Meat Production

Fabrication and Investigation of Arrayed Glucose Biosensor Based on Microfluidic Framework

Enzyme SU-8 microreactors: simple tools for cell-culture monitoring

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