Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach

Ireta-Muñoz, Laura Alejandra; Morales‐Narváez, Eden

doi:10.3390/bios10060060

Cited by 12 publications

(9 citation statements)

References 22 publications

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“…Herein, the PhotoBox could be extended by an excitation light and emission filter that allows for GFP detection using a smartphone. A low-cost and easy approach using paperbased filters in combination with a smartphone for a fluorescence readout has already been implemented 19 . Finally, automated sample illumination and evaluation of the results via a smartphone app could be realized for user friendly operation.…”

Section: Discussionmentioning

confidence: 99%

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OptoAssay – Light-controlled Dynamic Bioassay Using Optogenetic Switches

Urban

Hörner

Weber

et al. 2021

Preprint

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Circumventing the limitations of current bioassays, we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics. Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light. Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.

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Section: Discussionmentioning

confidence: 99%

“…Herein, the PhotoBox could be extended by an excitation light and emission filter that allows for GFP detection using a smartphone. Various low-cost and easy sensing approaches in combination with a smartphone for a fluorescence readout have already been implemented 20 .…”

Section: Discussionmentioning

confidence: 99%

OptoAssay – Light-controlled Dynamic Bioassay Using Optogenetic Switches

Urban

Hörner

Weber

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…This includes not only protocols adopting conventional fluorescence plate readers ( Sun et al., 2021 ; Tsou et al., 2021 ), but also strategies suited for measuring fluorescence at the point of care. Portable, miniaturized systems for detection of fluorescence have been developed, which enable nucleic acid detection in this second case ( Ganguli et al., 2020 ; González-González et al., 2020 ; Ireta-Muñoz and Morales-Narváez, 2020 ; Samacoits et al., 2021 ) ( Table 7 ). In addition, there are approaches based on UV-Vis spectrophotometry ( Health, 2021 ), which also demand the use of plate-readers, or in the detection of chemiluminescence, which require the use of a luminometer ( Inc, 2021 ) ( Table 7 ).…”

Section: Platforms For Performing Nucleic Acids Detectionmentioning

confidence: 99%

SARS-CoV-2 Diagnostics Based on Nucleic Acids Amplification: From Fundamental Concepts to Applications and Beyond

Vindeirinho

Pinho

Azevedo

et al. 2022

Front. Cell. Infect. Microbiol.

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COVID-19 pandemic ignited the development of countless molecular methods for the diagnosis of SARS-CoV-2 based either on nucleic acid, or protein analysis, with the first establishing as the most used for routine diagnosis. The methods trusted for day to day analysis of nucleic acids rely on amplification, in order to enable specific SARS-CoV-2 RNA detection. This review aims to compile the state-of-the-art in the field of nucleic acid amplification tests (NAATs) used for SARS-CoV-2 detection, either at the clinic level, or at the Point-Of-Care (POC), thus focusing on isothermal and non-isothermal amplification-based diagnostics, while looking carefully at the concerning virology aspects, steps and instruments a test can involve. Following a theme contextualization in introduction, topics about fundamental knowledge on underlying virology aspects, collection and processing of clinical samples pave the way for a detailed assessment of the amplification and detection technologies. In order to address such themes, nucleic acid amplification methods, the different types of molecular reactions used for DNA detection, as well as the instruments requested for executing such routes of analysis are discussed in the subsequent sections. The benchmark of paradigmatic commercial tests further contributes toward discussion, building on technical aspects addressed in the previous sections and other additional information supplied in that part. The last lines are reserved for looking ahead to the future of NAATs and its importance in tackling this pandemic and other identical upcoming challenges.

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“…Recently, several studies have demonstrated applications of the PADs to different types of food and environmental samples using detection schemes such as colorimetry, − electrochemistry, − fluorometry, − and chemiluminescence. − Colorimetry is the most frequently employed detection method because it is simple to achieve qualitative and quantitative analyses of analytes by observing the color change in the detection zones on the PAD. The color images are captured with a digital camera, smartphone, or scanner, which then is followed by image processing using software such as ImageJ. , …”

Section: Introductionmentioning

confidence: 99%

Development of Pipetteless Paper-Based Analytical Devices with a Volume Gauge

et al. 2023

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In this work, we propose a new design for paperbased analytical devices (PADs) that eliminate the need to use a micropipette for sample introduction. With this design, a PAD is equipped with a distance-based detection channel that is connected to a storage channel that indicates the volume of a sample introduced into the PAD. The analyte in the sample solution reacts with a colorimetric reagent deposited into the distance-based detection channel as the sample solution flows into the storage channel where the volume is measured. The ratio of the lengths of the detection channel and that of the storage channel (D/S ratio) are constant for a sample containing a certain concentration, which is independent of the introduced volume. Therefore, the PADs permit volume-independent quantification using a dropper instead of a micropipette because the length of the storage channel plays the role of a volume gauge to estimate the introduced sample volume. In this study, the D/S ratios obtained with a dropper were comparable to those obtained with a micropipette, which confirmed that precise volume control is unnecessary for this PAD system. The proposed PADs were applied to the determinations of iron and bovine serum albumin using bathophenanthroline and tetrabromophenol blue as colorimetric reagents, respectively. The calibration curves showed good linear relationships with coefficients of 0.989 for iron and 0.994 for bovine serum albumin, respectively.

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Smartphone and Paper-Based Fluorescence Reader: A Do It Yourself Approach

Cited by 12 publications

References 22 publications

OptoAssay – Light-controlled Dynamic Bioassay Using Optogenetic Switches

OptoAssay – Light-controlled Dynamic Bioassay Using Optogenetic Switches

SARS-CoV-2 Diagnostics Based on Nucleic Acids Amplification: From Fundamental Concepts to Applications and Beyond

Development of Pipetteless Paper-Based Analytical Devices with a Volume Gauge

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