Smartphone-Based Dual-Modality Imaging System for Quantitative Detection of Color or Fluorescent Lateral Flow Immunochromatographic Strips

Hou, Yanren; Wang, Kan; Xiao, Kun; Qin, Wei; Lu, Wenting; Tao, Wei; Cui, Daxiang

doi:10.1186/s11671-017-2078-9

Cited by 61 publications

(21 citation statements)

References 36 publications

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“…The visual detection limit of the LFIAs is 10 ng/g. Additionally, we employed a portable smartphone strip reader with multiple image processing to analyse strips in 3 s [19,21]. The LOD is 0.3 ng/g.…”

Section: Comparison Between Hplc and Immunochromatographic Strip For mentioning

confidence: 99%

“…A fluorescence detection apparatus that was coupled to a desktop computer or laptop, enabling rapid processing speeds and stable performances, recorded the fluorescence intensity of the dot. However, these bulky and heavy devices limit their widespread application in the field of family and personal care [19][20][21]. Alternatively, a mobile device-based strip reader could satisfy the requirement of high portability and feature-rich testing.…”

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confidence: 99%

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Developing Gold Nanoparticles-Conjugated Aflatoxin B1 Antifungal Strips

Sojinrin

Liu

Wang

et al. 2019

IJMS

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Lateral flow immunochromatographic assays are a powerful diagnostic tool for point-of-care tests, based on their simplicity, specificity, and sensitivity. In this study, a rapid and sensitive gold nanoparticle (AuNP) immunochromatographic strip is produced for detecting aflatoxin B1 (AFB1) in suspicious fungi-contaminated food samples. The 10 nm AuNPs were encompassed by bovine serum albumin (BSA) and AFB1 antibody. Thin-layer chromatography, gel electrophoresis and nuclear magnetic resonance spectroscopy were employed for analysing the chemical complexes. Various concentrations of AFB1 antigen (0-16 ng/mL) were tested with AFB1 antibody-BSA-AuNPs (conjugated AuNPs) and then analysed by scanning electron microscopy, ultraviolet-visible spectroscopy, and Zetasizer. The results showed that the AFB1 antibody was coupled to BSA by the N-hydroxysuccinimide ester method. The AuNPs application has the potential to contribute to AFB1 detection by monitoring a visible colour change from red to purple-blue, with a detection limit of 2 ng/mL in a 96-well plate. The lateral flow immunochromatographic strip tests are rapid, taking less than 10 min., and they have a detection capacity of 10 ng/g. The smartphone analysis of strips provided the results in 3 s, with a detection limit of 0.3 ng/g for AFB1 when the concentration was below 10 ng/g. Excellent agreement was found with AFB1 determination by high-performance liquid chromatography in the determination of AFB1 among 20 samples of peanuts, corn, rice, and bread.for Aflatoixin B1 (AFB1) [8]. The European Economic Community (EEC) has established permitted food contamination limits of 2 µg/kg for AFB1 and 4 µg/kg for the total concentration of the four AFLs since 1 February 1999 [9]. Therefore, it is necessary to develop strategies for achieving the limits of AFL contamination and reducing AFL exposure in vulnerable populations [10].Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) are the most popular techniques for detecting AFLs. However, these methods require extensive sample preparation, expensive instruments, and operation by skilled professionals. Alternatively, the enzyme-linked immunosorbent assay (ELISA) has been successfully developed for AFLs [11], but ELISA also needs incubation and washing steps, and application is mainly confined to laboratories. Lateral flow immunochromatographic/immunoassay strips (LFIAs) have received increasing attention for qualitative and quantitative analysis in different scientific sectors [12], including food safety, environmental monitoring, and precision medicine [12,13]. In 2005, Delmulle et al. [14] developed an LFIA for the detection of aflatoxin B1 (AFB1) in pig feed. Liao and Li [15] have made significant effort to investigate the effect of the core-shell silver-gold nanocomposites on the properties of LFIAs. However, this detection can only provide either qualitative (positive or negative) or semi-quantitative information on analyte concentration, and thereby does not satisfy the requirements for...

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Section: Comparison Between Hplc and Immunochromatographic Strip For mentioning

confidence: 99%

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confidence: 99%

Developing Gold Nanoparticles-Conjugated Aflatoxin B1 Antifungal Strips

Sojinrin

Liu

Wang

et al. 2019

IJMS

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“…Recently, smartphone-based dual-modality imaging system [45] was developed by us, which could quantitativly detect color or fluorescent lateral flow test strips and be operated anywhere at any time, Fig. 9.…”

Section: Research On the Detection Systemmentioning

confidence: 99%

The Application of Lateral Flow Immunoassay in Point of Care Testing: A Review

Wang¹,

Qin²,

Hou³

et al. 2016

Nano BioMed ENG

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Point-of-care testing (POCT) is essential for providing a rapid diagnostic result in a prompt onsite diagnosis and treatment. A quick analysis time and a high sensitivity, with a sample-to-answer format, are the most important features for current POCT diagnostic systems. This review covers recent advances in POCT technologies with an emphasis on demonstrated and commercially available POCT diagnostic systems with laboratory quality using lateral flow immunoassay (LFIA). The system includes the integration of nanoparticles (NPs) in lateral flow test strips (LFTSs) and the mechanism through which particles improve the analytical performance of the fabricated strips. Several examples of NP-based LFTSs were selected to illustrate novel concepts or devices with promising applications as screening tools and superior alternatives to existing conventional strategies in clinical analysis, food safety, and environmental monitoring. In each analyte category, detection methods, configuration of LFIA modules, and advantages of POCT systems are reviewed and discussed along with future prospects. This review also discusses novel signal-enhancement strategies, optimal reader systems, and multiplex design prototypes, which have been employed for highly sensitive multiplex assay of LFTSs.

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“…Ling et al reported a lateral flow-through strip for use with a smartphone camera that could quantitatively detect alkaline phosphatase activity in milk [31]. Hou et al developed a smartphone-based, dual-modality imaging system that quantitatively detected either color or fluorescent LFIS [32]. In previous reports, smartphone-based LFIS readers were used directly with smartphone cameras to capture images of the LFIS test results.…”

Section: Introductionmentioning

confidence: 99%