Smartphone-based fluorescent lateral flow immunoassay platform for highly sensitive point-of-care detection of Zika virus nonstructural protein 1

Rong, Zhen; Wang, Qiong; Sun, Nanxi; Jia, Xiaofei; Wang, Keli; Xiao, Rui; Wang, Shengqi

doi:10.1016/j.aca.2018.12.043

Cited by 151 publications

(97 citation statements)

References 50 publications

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“…A good example that results from this new multidisciplinary approach is the smartphones-based POC devices, as previously cited. They are a real and promising novel tool for flaviviruses detection without complex instruments, since, in a simple way, the blood sample can be analyzed under 40 min (Priye et al, 2017;Rong et al, 2019). Despite the advances, still, there is an urgent need for proper and precise use of nanosensors in hospitals, field, and to prevent these potential health risk diseases.…”

Section: Biosensorsmentioning

confidence: 99%

Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases

Campos

Oliveira

Abrantes

et al. 2020

Front. Bioeng. Biotechnol.

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Arboviruses such as yellow fever, dengue, chikungunya and zika are transmitted mainly by the mosquito vector Aedes aegypti. Especially in the tropics, inefficacy of mosquito control causes arboviruses outbreaks every year, affecting the general population with debilitating effects in infected individuals. Several strategies have been tried to control the proliferation of A. aegypti using physical, biological, and chemical control measures. Other methods are currently under research and development, amongst which the use of nanotechnology has attracted a lot of attention of the researchers in relation to the production of more effective repellents and larvicides with less toxicity, and development of rapid sensors for the detection of virus infections. In this review, the utilization of nano-based formulations on control and diagnosis of mosquito-borne diseases were discussed. We also emphasizes the need for future research for broad commercialization of nano-based formulations in world market aiming a positive impact on public health.

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Section: Biosensorsmentioning

confidence: 99%

Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases

Campos

Oliveira

Abrantes

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Further, NGS platforms like MinION (a portable, real-time NGS sequencer) coupled with NanoPipe analysis are promising tools to perform bacterial and viral disease investigation in low throughput laboratories and specifically in the field (Beato et al, 2018;Shabardina et al, 2019). Although, yet not been adopted for animal disease diagnosis, but novel platforms such as smartphonebased diagnosis (which expands nucleic acid-based detection assays toward POCD) like RT-LAMP and fluorescent lateral flow immunoassay (already developed for Zika virus and Dengue virus) provide exciting opportunities for veterinary diagnostics in the near future (Rong et al, 2019).…”

Section: Biotechnological Tools In Companion Animals' Disease Diagnosismentioning

confidence: 99%

Biotechnological innovations in farm and pet animal disease diagnosis

Malik

Verma

Kumar

et al. 2020

Genomics and Biotechnological Advances in Veterinary, Poultry, and Fisheries

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“…IgG and IgM antibodies are produced in the human body at the later stage of the infection (four to seven days), this makes them inapt for early stage detection. In contrast, NS1 antigen with a similar structure has been considered for highly sensitive, specific early stage detection for different flaviviruses previously [9][10][11][12][13][14]. There are currently numerous POC devices that detect ZIKV by using NS1 as a biomarker.…”

Section: Introductionmentioning

confidence: 99%

“…Although the technique lowered the cost and sensitivity, the assay is not suitable for point-of-care applications. Paper-based lateral flow immunoassay (LFIA) devices also exist, which can be effective in detecting infections/molecules [11,[15][16][17][18]. Even though LFIA is cheap, rapid, and portable, it can only give qualitative or semiqualitative results.…”

Section: Introductionmentioning

confidence: 99%

Development of a Flow-Free Automated Colorimetric Detection Assay Integrated with Smartphone for Zika NS1

Kabir

Zilouchian²,

Sher

et al. 2020

Diagnostics

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The Zika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitoes that can potentially cause microcephaly, Guillain–Barré Syndrome, and other birth defects. Effective vaccines for Zika have not yet been developed. There is a necessity to establish an easily deployable, high-throughput, low-cost, and disposable point-of-care (POC) diagnostic platform for ZIKV infections. We report here an automated magnetic actuation platform suitable for a POC microfluidic sandwich enzyme-linked immunosorbent assay (ELISA) using antibody-coated superparamagnetic beads. The smartphone integrated immunoassay is developed for colorimetric detection of ZIKV nonstructural protein 1 (NS1) antigen using disposable chips to accommodate the reactions inside the chip in microliter volumes. An in-house-built magnetic actuator platform automatically moves the magnetic beads through different aqueous phases. The assay requires a total of 9 min to automatically control the post-capture washing, horseradish peroxidase (HRP) conjugated secondary antibody probing, washing again, and, finally, color development. By measuring the saturation intensity of the developed color from the smartphone captured video, the presented assay provides high sensitivity with a detection limit of 62.5 ng/mL in whole plasma. These results advocate a great promise that the platform would be useful for the POC diagnosis of Zika virus infection in patients and can be used in resource-limited settings.

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Smartphone-based fluorescent lateral flow immunoassay platform for highly sensitive point-of-care detection of Zika virus nonstructural protein 1

Cited by 151 publications

References 50 publications

Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases

Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases

Biotechnological innovations in farm and pet animal disease diagnosis

Development of a Flow-Free Automated Colorimetric Detection Assay Integrated with Smartphone for Zika NS1

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