Smartphone-based kanamycin sensing with ratiometric FRET

Umrao, Saurabh; Anusha, S.; Jain, Vasundhara; Chakraborty, Biswanath; Roy, Rahul

doi:10.1039/c8ra10035g

Cited by 18 publications

(10 citation statements)

References 58 publications

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“…In addition to the change in intensity of single-color fluorescence, the analytes can be detected by the signal conversion of two-color fluorescence. Saurabh et al [ 60 ] demonstrated ratiometric Förster resonance energy transfer (FRET) between fluorescent dye pairs on aptamers for rapid-response and sensitive kanamycin detection. As depicted in Figure 2 d, the upper half of dye-labeled kanamycin DNA aptamer (KBA) is the kanamycin binding aptamer, while the bottom is double-stranded DNA used for surface modification of glass slide.…”

Section: Smartphone-based Aptasensormentioning

confidence: 99%

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

Tan

et al. 2022

Biosensors

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Aptamers are a particular class of functional recognition ligands with high specificity and affinity to their targets. As the candidate recognition layer of biosensors, aptamers can be used to sense biomolecules. Aptasensors, aptamer-based biosensors, have been demonstrated to be specific, sensitive, and cost-effective. Furthermore, smartphone-based devices have shown their advantages in binding to aptasensors for point-of-care testing (POCT), which offers an immediate or spontaneous responding time for biological testing. This review describes smartphone-based aptasensors to detect various targets such as metal ions, nucleic acids, proteins, and cells. Additionally, the focus is also on aptasensors-related technologies and configurations.

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Section: Smartphone-based Aptasensormentioning

confidence: 99%

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

Tan

et al. 2022

Biosensors

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“…When EpCAM is present, it displaces the Cy3-DNA conjugate from the aptamer, resulting in a loss of FRET signal ( Figure 4 g). Multiple biosensors can be delivered in this way at the PON, including smaller aptasensors, which have been used to detect antibiotics in milk [ 88 ]; however, these approaches have not yet been adopted commercially.…”

Section: Pon-suitable Ret Applicationsmentioning

confidence: 99%

“…The connector oligonucleotide is conjugated to Cy5. Binding of kanamycin spatially separates Cy3 from Cy5 components, leading to a lower FRET efficiency [ 88 ]. ( h ) An upconversion nanoparticle (UCNP) consisting of ytterbium (Yb 3+ ) and thulium (Tm 3+ ) is conjugated to the organic dye rhodol.…”

Section: Figurementioning

confidence: 99%

Resonance Energy Transfer-Based Biosensors for Point-of-Need Diagnosis—Progress and Perspectives

Weihs

Anderson

Trowell

et al. 2021

Sensors

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The demand for point-of-need (PON) diagnostics for clinical and other applications is continuing to grow. Much of this demand is currently serviced by biosensors, which combine a bioanalytical sensing element with a transducing device that reports results to the user. Ideally, such devices are easy to use and do not require special skills of the end user. Application-dependent, PON devices may need to be capable of measuring low levels of analytes very rapidly, and it is often helpful if they are also portable. To date, only two transduction modalities, colorimetric lateral flow immunoassays (LFIs) and electrochemical assays, fully meet these requirements and have been widely adopted at the point-of-need. These modalities are either non-quantitative (LFIs) or highly analyte-specific (electrochemical glucose meters), therefore requiring considerable modification if they are to be co-opted for measuring other biomarkers. Förster Resonance Energy Transfer (RET)-based biosensors incorporate a quantitative and highly versatile transduction modality that has been extensively used in biomedical research laboratories. RET-biosensors have not yet been applied at the point-of-need despite its advantages over other established techniques. In this review, we explore and discuss recent developments in the translation of RET-biosensors for PON diagnoses, including their potential benefits and drawbacks.

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“…In the absence of kanamycin, a high FRET efficiency between Cy3 and Cy5 was obtained, while the binding of kanamycin to the aptamer led to a low FRET efficiency. A homemade smartphone setup was used to obtain the ratio FL signal and showed a LOD of 28 nM for kanamycin [39].…”

Section: Ratio Fluorescent Analysis Strategiesmentioning

confidence: 99%

“…Representative (A) turn-on (reprinted with permission from[37] Copyright (2018) Elsevier) and (B) ratio FL detection for kanamycin based on fluorescence resonance energy transfer (FRET) (reproduced with permission from[39] Copyright (2019) Royal Society of Chemistry).…”

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confidence: 99%

Recent Advances in Optical Detection of Aminoglycosides

Zhou

Cao

2020

Applied Sciences

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Aminoglycosides (AGs) are broad-spectrum antibiotics used in both human infection and animal medicine. The overuse of AGs causes undesirable residues in food, leading to serious health problems due to food chain accumulation. In recent years, various methods have been developed to determine AGs in food. Among these methods, fluorescent (FL), colorimetric and chemiluminescent (CL) optical methods possess advantages such as their simple instrumentation, low cost, simple operation, feasibility of realizing visualization, and smartphone imaging. This mini-review summarizes optical assays for the detection of AGs in food developed in recent years. The detection principles for different categories are discussed. Then, the amplification techniques for the ultrasensitive detection of AGs are introduced. We also discuss multiplex methods for the simultaneous detection of AGs. Finally, the challenges and future prospects are discussed in the Conclusions and Perspectives section.

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Smartphone-based kanamycin sensing with ratiometric FRET

Abstract: FRET aptamer based kanamycin detection enables reusable and smartphone sensing.

Cited by 18 publications

References 58 publications

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

Applications of Smartphone-Based Aptasensor for Diverse Targets Detection

Resonance Energy Transfer-Based Biosensors for Point-of-Need Diagnosis—Progress and Perspectives

Recent Advances in Optical Detection of Aminoglycosides

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