Smartphone-based Wireless Operated Milliliter-scale Bioreactor

Abstract

<p>This paper presents the development of a smartphone-based wireless operated milliliter scale bioreactor. The bioreactor has a working volume of 18 mL and is made of poly (methylmethacrylate) (PMMA) and poly (dimethylsiloxane) (PDMS) polymers using a laser engraving methodology. Temperature and reactor stirring speed are the controlled variables and wireless communication is achieved through a wireless transceiver embedded in a low-cost controller platform. The results attained are very promising as the controller is able to control the desired reactor variables precisely through wireless communications.</p>

A portable sensor for cell optical density measurement in microfluidic chips

“…The starting pH of the YPD medium was adjusted to pH 6 using 2 M sulfuric acid, H 2 SO 4 , and 2 M sodium hydroxide, NaOH solutions. The reactor was sparged with air at 100 µL h –1 for oxygenation and the stirring rate was set at 90 r/min to achieve a mixing time of about 6 s. 20 Sterilization of the reactor was performed in two steps. First, the reactor was cleaned with a 70% (v/v) ethanol solution before thoroughly rinsing it with distilled water several times.…”

“…The reactor has a working volume of approximately 18 mL and features various feedback control loops including stirring, temperature, and aeration. Details of the reactor design and fabrication have been described in Whyte et al 20 The bioreactor system was modified to include a miniature probe for measuring cell OD where light from a LED is transmitted into the bioreactor and then sent to a PD by using optical fibers ( Figure 3). The optical fiber for light transmittance was mounted at the bottom of the reactor, while the receiving fiber was fixed into lid of the reactor, immersed in the solution.…”