Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay

Chen, Arnold; Wang, Royal; Bever, Candace S.; Xing, Siyuan; Hammock, Bruce D.; Pan, Tingrui

doi:10.1063/1.4901348

Cited by 61 publications

(44 citation statements)

References 59 publications

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“…Severe air pollution, for example, is linked to respiratory tract infections and chronic obstructive pulmonary disease, and contaminated food and water has often induced diarrheal diseases [65]. A plug-and-play microfluidic ELISA chip was designed to analyze the presence of BDE-47 (2,20,4,40-tetrabromodiphenyl ether) [66]. Studies showed that long-term exposure to BDE-47, a common type of polybrominated diphenyl ethers (PBDEs) as flame retardant in consumer products, significantly deteriorated cognitive function and motor behavior in infants ( Figure 8B) [67].…”

Section: Immunoassaymentioning

confidence: 99%

Cellphone-based diagnostic devices

Huang¹,

Cheng²

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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Section: Immunoassaymentioning

confidence: 99%

Cellphone-based diagnostic devices

Huang¹,

Cheng²

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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“…Another device under development is a low-cost portable smart phone-enabled device to detect the presence and concentrations of the flame retardant BDE-47 (Table 1K). The device uses a lab-on-a-chip (LOC) platform to perform microscale enzyme-linked immunosorbent assays (ELISA) (16).…”

Section: Personal Exposure Monitoringmentioning

confidence: 99%

Sustainable exposure prevention through innovative detection and remediation technologies from the NIEHS Superfund Research Program

Henry

Suk

2017

Reviews on Environmental Health

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Abstract Innovative devices and tools for exposure assessment and remediation play an integral role in preventing exposure to hazardous substances. New solutions for detecting and remediating organic, inorganic, and mixtures of contaminants can improve public health as a means of primary prevention. Using a public health prevention model, detection and remediation technologies contribute to primary prevention as tools to identify areas of high risk (e.g. contamination hotspots), to recognize hazards (bioassay tests), and to prevent exposure through contaminant cleanups. Primary prevention success is ultimately governed by the widespread acceptance of the prevention tool. And, in like fashion, detection and remediation technologies must convey technical and sustainability advantages to be adopted for use. Hence, sustainability – economic, environmental, and societal – drives innovation in detection and remediation technology. The National Institute of Health (NIH) National Institute of Environmental Health Sciences (NIEHS) Superfund Research Program (SRP) is mandated to advance innovative detection, remediation, and toxicity screening technology development through grants to universities and small businesses. SRP recognizes the importance of fast, accurate, robust, and advanced detection technologies that allow for portable real-time, on-site characterization, monitoring, and assessment of contaminant concentration and/or toxicity. Advances in non-targeted screening, biological-based assays, passive sampling devices (PSDs), sophisticated modeling approaches, and precision-based analytical tools are making it easier to quickly identify hazardous “hotspots” and, therefore, prevent exposures. Innovation in sustainable remediation uses a variety of approaches: in situ remediation; harnessing the natural catalytic properties of biological processes (such as bioremediation and phytotechnologies); and application of novel materials science (such as nanotechnology, advanced membranes, new carbon materials, and materials reuse). Collectively, the investment in new technologies shows promise to reduce the amount and toxicity of hazardous substances in the environment. This manuscript highlights SRP funded innovative devices and tools for exposure assessment and remediation of organic, inorganic, and mixtures of contaminants with a particular focus on sustainable technologies.

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“…Current applications of MS 2 cover detection of various environmental and health indicators such as pH (Lopez-Ruiz et al 2014), nitrite , heavy metal (Chen et al 2014b;Wang et al 2014), bacterial contamination (Hutchison et al 2015;San Park et al 2013;Zhu et al 2012), blood glucose (Chun et al 2014), proteins (Chan et al 2015;Lillehoj et al 2013;Preechaburana et al 2012;You et al 2013) and other pathogen-associated biomarkers (Fronczek et al 2014;Stemple et al 2014;Yeo et al 2016). Some complicated assays such as enzyme-linked immunosorbent assay (ELISA) (Chen et al 2014a;Wang et al 2011) and polymerase chain reaction (PCR) Liao et al 2016;Stedtfeld et al 2012) were successfully implemented with the MS 2 systems. Furthermore, MS 2 systems offers advantages in test speed, self-containment and sample to result assay operation, which are required for in field test and point of care (PoC) diagnosis (D'Ambrosio et al 2015;Hu et al 2016;Laksanasopin et al 2015;Mudanyali et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Mkit: A cell migration assay based on microfluidic device and smartphone

Yang

Peretz‐Soroka

et al. 2018

Biosensors and Bioelectronics

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Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS 2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS 2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS 2 -based cell functional assay for testing cell migration (the M kit ). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the M kit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the M kit with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the M kit . In addition to research applications, we demonstrated the effective use of the M kit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed M kit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.

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Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay

Cited by 61 publications

References 59 publications

Cellphone-based diagnostic devices

Cellphone-based diagnostic devices

Sustainable exposure prevention through innovative detection and remediation technologies from the NIEHS Superfund Research Program

Mkit: A cell migration assay based on microfluidic device and smartphone

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