SmdAB, a Heterodimeric ABC-Type Multidrug Efflux Pump, in
            <i>Serratia marcescens</i>

Matsuo, Tetsuji; Chen, Jing; Minato, Yusuke; Ogawa, Wakano; Mizushima, Tohru; Kuroda, Teruo; Tsuchiya, Tomofusa

doi:10.1128/jb.01513-07

Cited by 59 publications

(34 citation statements)

References 43 publications

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“…When expressed from plasmids, three additional pumps, SmdAB (a heterodimeric ABC-type), SmfY (an MFS-type) and SsmE (an SMR-type), confer, to a multidrug-susceptible E. coli , resistance to several structurally unrelated antibacterials (Table II). [202–204] TetA(41) efflux protein and TetR(41) repressor were observed in an environmental strain of S. marcescens . [205] A TetR/AcrR family repressor PizR, which was identified because of its effect on the production of secondary metabolites (prodigiosin and carbapenem), is a specific repressor of a four-component RND efflux pump, ZrpADBC.…”

Section: Drug Efflux In Gram-negative Bacteriamentioning

confidence: 99%

Efflux-Mediated Drug Resistance in Bacteria

Nikaido²

2009

Drugs

922

875

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Drug efflux pumps play a key role in drug resistance and also serve other functions in bacteria. There has been a growing list of multidrug and drug-specific efflux pumps characterized from bacteria of human, animal, plant and environmental origins. These pumps are mostly encoded on the chromosome although they can also be plasmid-encoded. A previous article (Li X-Z and Nikaido H, Drugs, 2004; 64[2]: 159–204) had provided a comprehensive review regarding efflux-mediated drug resistance in bacteria. In the past five years, significant progress has been achieved in further understanding of drug resistance-related efflux transporters and this review focuses on the latest studies in this field since 2003. This has been demonstrated in multiple aspects that include but are not limited to: further molecular and biochemical characterization of the known drug efflux pumps and identification of novel drug efflux pumps; structural elucidation of the transport mechanisms of drug transporters; regulatory mechanisms of drug efflux pumps; determining the role of the drug efflux pumps in other functions such as stress responses, virulence and cell communication; and development of efflux pump inhibitors. Overall, the multifaceted implications of drug efflux transporters warrant novel strategies to combat multidrug resistance in bacteria.

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Section: Drug Efflux In Gram-negative Bacteriamentioning

confidence: 99%

Efflux-Mediated Drug Resistance in Bacteria

Nikaido²

2009

Drugs

922

875

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“…Tetracycline is a substrate for the RND pump SdeXY (68). Matsuo and others showed that the ABC pump SmdAB provided increased tetracycline resistance when it was cloned into a susceptible E. coli strain (257). In addition, the RND pump SdeAB was shown to provide an increase in tetracycline resistance after S. marcescens was exposed to cetylpyridinium chloride (255).…”

Section: Resistance To the Tetracyclines In Serratia Speciesmentioning

confidence: 99%

“…Another efflux pump characterized from S. marcescens, SmdAB, belongs to the ATP-binding cassette (ABC) family (257). When cloned into E. coli, this pump provided elevated MICs for several antimicrobials, including the quinolones ciprofloxacin, norfloxacin, ofloxacin, and nalidixic acid (257). Efflux pumps have not been well characterized for other Serratia species, but several are predicted from the genome sequence of S. proteamaculans strain 568.…”

Section: Quinolone Resistance In Serratia Speciesmentioning

confidence: 99%

“…Interestingly, it was shown that exposure of S. marcescens to cetylpyridinium chloride, a quaternary ammonium disinfectant, caused mutations in SdeAB that increased resistance to norfloxacin, biocides, and several other antibiotics (255). Another efflux pump characterized from S. marcescens, SmdAB, belongs to the ATP-binding cassette (ABC) family (257). When cloned into E. coli, this pump provided elevated MICs for several antimicrobials, including the quinolones ciprofloxacin, norfloxacin, ofloxacin, and nalidixic acid (257).…”

Section: Quinolone Resistance In Serratia Speciesmentioning

confidence: 99%

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Serratia Infections: from Military Experiments to Current Practice

2011

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SUMMARY Serratia species, in particular Serratia marcescens , are significant human pathogens. S. marcescens has a long and interesting taxonomic, medical experimentation, military experimentation, and human clinical infection history. The organisms in this genus, particularly S. marcescens , were long thought to be nonpathogenic. Because S. marcescens was thought to be a nonpathogen and is usually red pigmented, the U.S. military conducted experiments that attempted to ascertain the spread of this organism released over large areas. In the process, members of both the public and the military were exposed to S. marcescens , and this was uncovered by the press in the 1970s, leading to U.S. congressional hearings. S. marcescens was found to be a certain human pathogen by the mid-1960s. S. marcescens and S. liquefaciens have been isolated as causative agents of numerous outbreaks and opportunistic infections, and the association of these organisms with point sources such as medical devices and various solutions given to hospitalized patients is striking. Serratia species appear to be common environmental organisms, and this helps to explain the large number of nosocomial infections due to these bacteria. Since many nosocomial infections are caused by multiply antibiotic-resistant strains of S. marcescens , this increases the danger to hospitalized patients, and hospital personnel should be vigilant in preventing nosocomial outbreaks due to this organism. S. marcescens , and probably other species in the genus, carries several antibiotic resistance determinants and is also capable of acquiring resistance genes. S. marcescens and S. liquefaciens are usually identified well in the clinical laboratory, but the other species are rare enough that laboratory technologists may not recognize them. 16S rRNA gene sequencing may enable better identification of some of the less common Serratia species.

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“…Recently, a new heterodimeric ABC transporter from this microorganism, SmdAB, was cloned, and the plasmid harboring the genes was able to confer resistance to several agents in a hypersensitive strain of E. coli (307). Lactobacillus brevis is a major contaminant in spoiled beer, despite the presence of bacteriostatic compounds derived from the hop plant that are added to beer to give a bitter taste.…”

Section: Vol 72 2008 Bacterial Atp-binding Cassette Systems 343mentioning

confidence: 99%

Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems

Davidson

Dassa

Orelle

et al. 2008

Microbiol Mol Biol Rev

1,187

1,137

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SUMMARY ATP-binding cassette (ABC) systems are universally distributed among living organisms and function in many different aspects of bacterial physiology. ABC transporters are best known for their role in the import of essential nutrients and the export of toxic molecules, but they can also mediate the transport of many other physiological substrates. In a classical transport reaction, two highly conserved ATP-binding domains or subunits couple the binding/hydrolysis of ATP to the translocation of particular substrates across the membrane, through interactions with membrane-spanning domains of the transporter. Variations on this basic theme involve soluble ABC ATP-binding proteins that couple ATP hydrolysis to nontransport processes, such as DNA repair and gene expression regulation. Insights into the structure, function, and mechanism of action of bacterial ABC proteins are reported, based on phylogenetic comparisons as well as classic biochemical and genetic approaches. The availability of an increasing number of high-resolution structures has provided a valuable framework for interpretation of recent studies, and realistic models have been proposed to explain how these fascinating molecular machines use complex dynamic processes to fulfill their numerous biological functions. These advances are also important for elucidating the mechanism of action of eukaryotic ABC proteins, because functional defects in many of them are responsible for severe human inherited diseases.

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SmdAB, a Heterodimeric ABC-Type Multidrug Efflux Pump, in Serratia marcescens

Cited by 59 publications

References 43 publications

Efflux-Mediated Drug Resistance in Bacteria

Efflux-Mediated Drug Resistance in Bacteria

Serratia Infections: from Military Experiments to Current Practice

Structure, Function, and Evolution of Bacterial ATP-Binding Cassette Systems

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