Smelling Pseudomonas aeruginosa infections using a whole-cell biosensor – An alternative for the gold-standard culturing assay

Kviatkovski, Igor; Shushan, Sagit; Oron, Yahav; Frumin, Idan; Amir, Daniel; Secundo, Lavi; Livne, Eitan; Weissbrod, Aharon; Sobel, Noam; Helman, Yael

doi:10.1016/j.jbiotec.2017.12.023

Cited by 12 publications

(17 citation statements)

References 26 publications

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“…This was accomplished using a biosensor for 2-aminoacetophenone that was applied to pus samples from patients. The results were promising for further development of an easy-to-use device that is specific for P. aeruginosa ear infection (Kviatkovski et al, 2018).…”

Section: Promising Areas Of Research In Bacterial Infection Volatilomementioning

confidence: 92%

Volatilomes of Bacterial Infections in Humans

Elmassry

Piechulla

2020

Front. Neurosci.

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Sense of smell in humans has the capacity to detect certain volatiles from bacterial infections. Our olfactory senses were used in ancient medicine to diagnose diseases in patients. As humans are considered holobionts, each person's unique odor consists of volatile organic compounds (VOCs, volatilome) produced not only by the humans themselves but also by their beneficial and pathogenic micro-habitants. In the past decade it has been well documented that microorganisms (fungi and bacteria) are able to emit a broad range of olfactory active VOCs [summarized in the mVOC database (http://bioinformatics.charite.de/mvoc/)]. During microbial infection, the equilibrium between the human and its microbiome is altered, followed by a change in the volatilome. For several decades, physicians have been trying to utilize these changes in smell composition to develop fast and efficient diagnostic tools, particularly because volatiles detection is non-invasive and non-destructive, which would be a breakthrough in many therapies. Within this review, we discuss bacterial infections including gastrointestinal, respiratory or lung, and blood infections, focusing on the pathogens and their known corresponding volatile biomarkers. Furthermore, we cover the potential role of the human microbiota and their volatilome in certain diseases such as neurodegenerative diseases. We also report on discrete mVOCs that affect humans.

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Section: Promising Areas Of Research In Bacterial Infection Volatilomementioning

confidence: 92%

Volatilomes of Bacterial Infections in Humans

Elmassry

Piechulla

2020

Front. Neurosci.

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“…Pa and Af are the most important bacterium and fungus in the microbiota in many clinical settings, particularly in the lungs of immunocompromised persons and those with CF. Advanced technology has been used to detect the volatiles of Pa and other microbes [ 57 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 ], and such technology is currently under study as methods for the noninvasive detection of these pathogens [ 66 , 72 , 73 , 74 , 75 , 76 ]. Nearly 400 volatiles have been described from Pa, of which ketones are the most highly represented [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Aspergillus Is Inhibited by Pseudomonas aeruginosa Volatiles

Nazik

Sass

Déziel

et al. 2020

JoF

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Background: Pseudomonas aeruginosa (Pa) and Aspergillus fumigatus (Af) compete with each other for nutrients and survival in natural environments, and have been extensively studied because of their intermicrobial interactions in the human microbiome. These are the principal microbes infecting immunocompromised patients and persons with cystic fibrosis, particularly the airways. These intermicrobial studies have largely been conducted in liquid medium or on agar, and thus focus on soluble or diffusible microbial products. Several key inhibitory molecules were defined in such studies. Methods: in the present report, we examine several methodologies which can be conveniently used to study the interaction of microbial volatiles, including capture methods and kinetics. Results: Pa volatiles inhibit Af, and the inhibitory mechanism appears to be the incorporation of the inhibitory molecules into the substrate nourishing the Af, rather than directly onto Af structures. We define by mass spectroscopy some specific volatile Pa products that can inhibit Af. Some of these molecules are selected for interest by the study of gene deletion mutants, producing a few Pa strains that were impaired in inhibition. We presumed the volatiles of these latter strains could be excluded from the search for inhibitors. Conclusion: the Pa inhibition of Af via a gaseous phase could be critical components in their competition, particularly in airways, where more direct contact may not be extensive.

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“…[ 15 ] In order to prevent the occurrence of this situation, it is usually impossible to apply these GMO biosensors. [ 16,17 ] Furthermore, the response time of biosensors is prolonged because some analytes are hard to cross over the cell membrane, and some analytes may be toxic to living cells. Therefore, some cell‐based biosensors are not suitable for detecting cytotoxic or impermeable membrane analytes such as organophosphates.…”

Section: Introductionmentioning

confidence: 99%

Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications

Zhang

Guo

2020

Biotechnology Journal

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Synthetic biology has promoted the development of biosensors as tools for detecting trace substances. In the past, biosensors based on synthetic biology have been designed on living cells, but the development of cell biosensors has been greatly limited by defects such as genetically modified organism problem and the obstruction of cell membrane. However, the advent of cell‐free synthetic biology addresses these limitations. Biosensors based on the cell‐free protein synthesis system have the advantages of higher safety, higher sensitivity, and faster response time over cell biosensors, which make cell‐free biosensors have a broader application prospect. This review summarizes the workflow of various cell‐free biosensors, including the identification of analytes and signal output. The detection range of cell‐free biosensors is greatly enlarged by different recognition mechanisms and output methods. In addition, the review also discusses the applications of cell‐free biosensors in environmental monitoring and health diagnosis, as well as existing deficiencies and aspects that should be improved. In the future, through continuous improvement and optimization, the potential of cell‐free biosensors will be stimulated, and their application fields will be expanded.

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Smelling Pseudomonas aeruginosa infections using a whole-cell biosensor – An alternative for the gold-standard culturing assay

Cited by 12 publications

References 26 publications

Volatilomes of Bacterial Infections in Humans

Volatilomes of Bacterial Infections in Humans

Aspergillus Is Inhibited by Pseudomonas aeruginosa Volatiles

Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications

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