Smooth and Steady. How Lessons Learned Led to Successful Startup of the Chambers Creek Side Stream Deammonification Process

Klein, Adam; Summers, Amanda; Tobin, A.-E.; Johnson, Chandler; Melcer, Henryk

doi:10.2175/193864718825138970

Cited by 2 publications

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“…Together, these studies indicate that substrate composition and biofilm structure impose selective pressures that impact the heterotrophic microbial communities within PNA reactors. The reactors sampled in this study have been maintained under long-term stable operating conditions ( van Dongen et al, 2001 ; Abma et al, 2007 ; van der Star et al, 2007 ; Klein et al, 2018 ), suggesting that a stable microbial community has emerged under the unique environmental conditions imposed by each system.…”

Section: Discussionmentioning

confidence: 99%

“…Biomass and mixed liquor samples were collected from a one-stage PNA bioreactor at Chamber’s Creek Regional Wastewater Treatment Plant, Tacoma, WA, United States ( Klein et al, 2018 ), and a two-stage reactor in Olbergen, Rotterdam, the Netherlands ( van Dongen et al, 2001 ; Abma et al, 2007 ; van der Star et al, 2007 ). Material was separated by centrifugation at 5,000 rcf in a 50 mL tube resulting in three distinct layers: granules, flocs, and suspended fractions from the one-stage reactor, and only granules and suspended material from the two-stage system.…”

Section: Methodsmentioning

confidence: 99%

“…The primary distinguishing factor differentiating one-stage and two-stage PNA reactors is the presence of oxygen in the bulk liquid environment and this likely represents a significant factor in determining the available niches and the types of heterotrophic populations that are selected for. To improve our understanding of how reactor configuration impacts the microbial communities within PNA systems we characterized the microbial communities of a one-stage ( van Dongen et al, 2001 ; Abma et al, 2007 ; van der Star et al, 2007 ) and a two-stage system ( Klein et al, 2018 ) treating side-stream sludge anaerobic digester centrate. We performed 16S rRNA gene sequencing and shotgun metagenomic analyses to identify the microbial populations present in each system and assess structural and functional differences between the communities.…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic Insights Into Competition Between Denitrification and Dissimilatory Nitrate Reduction to Ammonia Within One-Stage and Two-Stage Partial-Nitritation Anammox Bioreactor Configurations

et al. 2022

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Anaerobic ammonia oxidizing bacteria (Anammox) are implemented in high-efficiency wastewater treatment systems operated in two general configurations; one-stage systems combine aerobic ammonia oxidizing bacteria (AOB) and Anammox within a single aerated reactor, whereas two-stage configurations separate these processes into discrete tanks. Within both configurations heterotrophic populations that perform denitrification or dissimilatory nitrate reduction to ammonia (DNRA) compete for carbon and nitrate or nitrite and can impact reactor performance because DNRA retains nitrogen in the system. Therefore, it is important to understand how selective pressures imposed by one-stage and two-stage reactor configurations impact the microbial community structure and associated nitrogen transforming functions. We performed 16S rRNA gene and metagenomic sequencing on different biomass fractions (granules, flocs, and suspended biomass) sampled from two facilities treating sludge dewatering centrate: a one-stage treatment facility (Chambers Creek, Tacoma, WA) and a two-stage system (Rotterdam, Netherlands). Similar microbial populations were identified across the different samples, but relative abundances differed between reactor configurations and biomass sources. Analysis of metagenome assembled genomes (MAGs) indicated different lifestyles for abundant heterotrophic populations. Acidobacteria, Bacteroidetes, and Chloroflexi MAGs had varying capacity for DNRA and denitrification. Acidobacteria MAGs possessed high numbers of glycosyl hydrolases and glycosyl transferases indicating a role in biomass degradation. Ignavibacteria and Phycosphaerae MAGs contributed to the greater relative abundance of DNRA associated nrf genes in the two-stage granules and contained genomic features suggesting a preference for an anoxic or microoxic niche. In the one-stage granules a MAG assigned to Burkholderiales accounted for much of the abundant denitrification genes and had genomic features, including the potential for autotrophic denitrification using reduced sulfur, that indicate an ability to adapt its physiology to varying redox conditions. Overall, the competition for carbon substrates between denitrifying and DNRA performing heterotrophs may be impacted by configuration specific selective pressures. In one-stage systems oxygen availability in the bulk liquid and the oxygen gradient within granules would provide a greater niche space for heterotrophic populations capable of utilizing both oxygen and nitrate or nitrite as terminal electron acceptors, compared to two-stage systems where a homogeneous anoxic environment would favor heterotrophic populations primarily adapted to anaerobic metabolism.

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