Smuggling gold nanoparticles across cell types – A new role for exosomes in gene silencing

Roma‐Rodrigues, Catarina; Pereira, Francisca; Matos, A. P. Alves de; Fernandes, Marta B.; Baptista, Pedro V.; Fernandes, Alexandra R.

doi:10.1016/j.nano.2017.01.013

Cited by 50 publications

(53 citation statements)

References 46 publications

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“…Delivery of TNAs, such as genes, oligonucleotides, miRNAs or siRNAs to cancer cells has allowed to tackle cancer via the silencing oncogenes or restoring the expression of tumor suppressor genes [8][9][10][11][12][13][14][15][16][17][18][19]. Most of these approaches (e.g., antisense therapy, RNA interference (RNAi), gene editing) aim at gene alteration/modulation [16][17][18][19][76][77][78][79][80][81] -see Figure 3. The immunization gene therapies, particularly chimeric antigen receptor (CAR) in T cells (CAR-T cells) based therapies, stand-out since they represent the higher number of therapeutic strategies in clinical practice.…”

Section: Gene Therapy Focused On Cancermentioning

confidence: 99%

“…They are composed by a lipid membrane and an exosomal lumen composed by proteins and nucleic acids, including mRNA and miRNAs, and their content is dependent of the cell of origin as well as in its physiological condition [142]. Importantly, after internalization by a secondary cell, exosomes induce phenotypical alterations dependent of the exosomal cargo [16,143,144]. Generally, tumor cells secrete higher quantities of exosomes than normal cells, and tumor cells derived exosomes promote tumor progression by inducing malignant transformation in normal cells, tumor escape to immune system, CAF transformation, angiogenesis and metastasis [7].…”

Section: Targeting Tumor Cells Derived Exosomesmentioning

confidence: 99%

“…The mounting knowledge on the characteristics of tumor cells and surrounding TME have sparked the use of gene therapy to tackle cancer molecular mechanisms. Gene therapy consists of the introduction of exogenous nucleic acids, such as genes, gene segments, oligonucleotides, miRNAs or siRNAs into cells envisaging a target gene edition, expression modulation of a target gene, mRNA or synthesis of an exogenous protein [8][9][10][11][12][13][14][15][16][17][18][19]. Gene transfer into tumor cells has been demonstrated via administration of therapeutic nucleic acids (TNAs) ex vivo and/or in vivo (Figure 1) [20].…”

Section: Introductionmentioning

confidence: 99%

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Gene Therapy in Cancer Treatment: Why Go Nano?

et al. 2020

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The proposal of gene therapy to tackle cancer development has been instrumental for the development of novel approaches and strategies to fight this disease, but the efficacy of the proposed strategies has still fallen short of delivering the full potential of gene therapy in the clinic. Despite the plethora of gene modulation approaches, e.g., gene silencing, antisense therapy, RNA interference, gene and genome editing, finding a way to efficiently deliver these effectors to the desired cell and tissue has been a challenge. Nanomedicine has put forward several innovative platforms to overcome this obstacle. Most of these platforms rely on the application of nanoscale structures, with particular focus on nanoparticles. Herein, we review the current trends on the use of nanoparticles designed for cancer gene therapy, including inorganic, organic, or biological (e.g., exosomes) variants, in clinical development and their progress towards clinical applications.

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Section: Gene Therapy Focused On Cancermentioning

confidence: 99%

Section: Targeting Tumor Cells Derived Exosomesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gene Therapy in Cancer Treatment: Why Go Nano?

et al. 2020

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“…Kasper et al found silica nanoparticles could decrease secretion of ICAM/E-selectin bearing exosomes/microvesicles when exposed to the inflamed endothelium [58] . Roma-Rodrigues et al found that gold nanoparticles (AuNPs) functionalized with thiolated oligonucleotides anti-RAB27A could decrease the release of exosomes due to specific gene silencing [59] . In addition, nanoparticles can also affect the sorting of exosomal cargos.…”

Section: Nanoparticlesmentioning

confidence: 99%

Exosomes in osteoarthritis and cartilage injury: advanced development and potential therapeutic strategies

et al. 2020

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Articular cartilage injury is a common clinical problem, which can lead to joint dysfunction, significant pain, and secondary osteoarthritis (OA) in which major surgical procedures are mandatory for treatment. Exosomes, as endosome-derived membrane-bound vesicles, participating in intercellular communications in both physiological and pathophysiological conditions, have been attached great importance in many fields. Recently, the significance of exosomes in the development of OA has been gradually concerned, while the therapeutic value of exosomes in cartilage repair and OA treatment has also been gradually revealed. The functional difference of different types and derivations of exosomes are determined by their specific contents. Herein, we provide comprehensive understanding on exosome and OA, including how exosomes participating in OA, the therapeutic value of exosomes for cartilage injury/OA, and related bioengineering strategies for future therapeutic design.

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“…71,73 AuNP@PEG@anti-RAB27A silence higher gene expression than that of RAB27A, also inhibiting formation of Rab27, a protein from RAB27A mRNA. 74 Xinyan Chen et al used "ship in a bottle strategy" and encapsulated AuNRs inside the hollow cavity of HSN (hollow silica nanoparticles). SF (hydrophobic antiproliferative and antiangiogenic drug) release was observed due to the detachment of CD-PGEA when NIR radiation was applied, leading to apoptosis in both in vitro as well as in vivo.…”

Section: Ayush Mongia Was Born Inmentioning

confidence: 99%