SMYD Proteins: Key Regulators in Skeletal and Cardiac Muscle Development and Function

Du, Shao Jun; Tan, Xungang; Zhang, Jianshe

doi:10.1002/ar.22972

Cited by 86 publications

(72 citation statements)

References 97 publications

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“…SMYD2 regulates HSP90 methylation50 and the inhibition of HSP90 has been shown to reduce AAA formation in murine models51, suggesting this as a possible link between SMYD2 and AAA. SMYD2 also plays a role in the differentiation of embryonic stem cells52, again suggesting a possible role for aberrations of in utero aortic development influencing the risk of aortic disease later in life.…”

Section: Discussionmentioning

confidence: 98%

Meta-Analysis of Genome-Wide Association Studies for Abdominal Aortic Aneurysm Identifies Four New Disease-Specific Risk Loci

Jones¹,

Tromp²,

Kuivaniemi³

et al. 2017

Circulation Research

179

195

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Rationale Abdominal aortic aneurysm (AAA) is a complex disease with both genetic and environmental risk factors. Together, 6 previously identified risk loci only explain a small proportion of the heritability of AAA. Objective To identify additional AAA risk loci using data from all available genome-wide association studies (GWAS). Methods and Results Through a meta-analysis of 6 GWAS datasets and a validation study totalling 10,204 cases and 107,766 controls we identified 4 new AAA risk loci: 1q32.3 (SMYD2), 13q12.11 (LINC00540), 20q13.12 (near PCIF1/MMP9/ZNF335), and 21q22.2 (ERG). In various database searches we observed no new associations between the lead AAA SNPs and coronary artery disease, blood pressure, lipids or diabetes. Network analyses identified ERG, IL6R and LDLR as modifiers of MMP9, with a direct interaction between ERG and MMP9. Conclusions The 4 new risk loci for AAA appear to be specific for AAA compared with other cardiovascular diseases and related traits suggesting that traditional cardiovascular risk factor management may only have limited value in preventing the progression of aneurysmal disease.

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Section: Discussionmentioning

confidence: 98%

Meta-Analysis of Genome-Wide Association Studies for Abdominal Aortic Aneurysm Identifies Four New Disease-Specific Risk Loci

Jones¹,

Tromp²,

Kuivaniemi³

et al. 2017

Circulation Research

179

195

View full text Add to dashboard Cite

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“…[12][13][14] The myogenic factor 6 (MYF6) gene belongs to the family of muscle regulatory factors (MRFs) and plays a major role in muscle growth and development. 15 MYF6 is the principal factor influencing the skeletal muscle phenotype.…”

Section: Discussionmentioning

confidence: 99%

Establishment and Cryopreservation of a Giant Panda Skeletal Muscle-Derived Cell Line

Zeng

Zhang

et al. 2015

Biopreservation and Biobanking

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The giant panda Ailuropoda melanoleuca is an endangered species and is a symbol for wildlife conservation. Although efforts have been made to protect this rare and endangered species through breeding and conservative biology, the long-term preservation of giant panda genome resources (gametes, tissues, organs, genomic libraries, etc.) is still a practical option. In this study, the giant panda skeletal muscle-derived cell line was successfully established via primary explants culture and cryopreservation techniques. The population doubling time of giant panda skeletal cells was approximately 33.8 h, and this population maintained a high cell viability before and after cryopreservation (95.6% and 90.7%, respectively). The two skeletal muscle-specific genes SMYD1 and MYF6 were expressed and detected by RT-PCR in the giant panda skeletal muscle-derived cell line. Karyotyping analysis revealed that the frequencies of giant panda skeletal muscle cells showing a chromosome number of 2n=42 ranged from 90.6∼94.2%. Thus, the giant panda skeletal muscle-derived cell line provides a vital resource and material platform for further studies and is likely to be useful for the protection of this rare and endangered species.

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“…Expression of two other muscle-enriched proteins, Smyd1, a member of a family of SET-MYND domain-containing lysine methyltransferases (10), which are critical for cardiac and skeletal muscle development (18), and Naca, whose musclespecific isoform, Sknac, is the major binding partner of Smyd1 in striated muscle (41), increased after removal of SB202190 from DM. Sknac knockout mice display impaired muscle growth and regeneration (41).…”

Section: Discussionmentioning

confidence: 99%

Separating myoblast differentiation from muscle cell fusion using IGF-I and the p38 MAP kinase inhibitor SB202190

Gardner

Gross

David

et al. 2015

American Journal of Physiology-Cell Physiology

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The p38 MAP kinases play critical roles in skeletal muscle biology, but the specific processes regulated by these kinases remain poorly defined. Here we find that activity of p38α/β is important not only in early phases of myoblast differentiation, but also in later stages of myocyte fusion and myofibrillogenesis. By treatment of C2 myoblasts with the promyogenic growth factor insulin-like growth factor (IGF)-I, the early block in differentiation imposed by the p38 chemical inhibitor SB202190 could be overcome. Yet, under these conditions, IGF-I could not prevent the later impairment of muscle cell fusion, as marked by the nearly complete absence of multinucleated myofibers. Removal of SB202190 from the medium of differentiating myoblasts reversed the fusion block, as multinucleated myofibers were detected several hours later and reached ∼90% of the culture within 30 h. Analysis by quantitative mass spectroscopy of proteins that changed in abundance following removal of the inhibitor revealed a cohort of upregulated muscle-enriched molecules that may be important for both myofibrillogenesis and fusion. We have thus developed a model system that allows separation of myoblast differentiation from muscle cell fusion and should be useful in identifying specific steps regulated by p38 MAP kinase-mediated signaling in myogenesis.

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SMYD Proteins: Key Regulators in Skeletal and Cardiac Muscle Development and Function

Cited by 86 publications

References 97 publications

Meta-Analysis of Genome-Wide Association Studies for Abdominal Aortic Aneurysm Identifies Four New Disease-Specific Risk Loci

Meta-Analysis of Genome-Wide Association Studies for Abdominal Aortic Aneurysm Identifies Four New Disease-Specific Risk Loci

Establishment and Cryopreservation of a Giant Panda Skeletal Muscle-Derived Cell Line

Separating myoblast differentiation from muscle cell fusion using IGF-I and the p38 MAP kinase inhibitor SB202190

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