SNAREs Regulate Vesicle Trafficking During Root Growth and Development

Luo, Changxin; Shi, Yumei; Xiang, Yun

doi:10.3389/fpls.2022.853251

Cited by 22 publications

(21 citation statements)

References 156 publications

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“…At present, the research on plant SNARE proteins is still in its infancy. Although a large number of SNARE homologous genes have been found in plants through genome sequence alignment, few of them have been biologically verified [ 40 , 41 ]. Transmembrane domain prediction analysis showed that HbOT1 and HbOT2 proteins had four and five transmembrane domains, respectively.…”

Section: Discussionmentioning

confidence: 99%

Cloning, Expression Analysis, and Functional Characterization of Candidate Oxalate Transporter Genes of HbOT1 and HbOT2 from Rubber Tree (Hevea brasiliensis)

Yang

Zhao

Peng

et al. 2022

Cells

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Secretion of oxalic acid from roots is an important aluminum detoxification mechanism for many plants such as Hevea brasiliensis (rubber tree). However, the underlying molecular mechanism and oxalate transporter genes in plants have not yet been reported. In this study, the oxalate transporter candidate genes HbOT1 and HbOT2 from the rubber tree were cloned and preliminarily identified. It was found that HbOT1 had a full length of 1163 bp with CDS size of 792 bp, encoding 263 amino acids, and HbOT2 had a full length of 1647 bp with a CDS region length of 840 bp, encoding 279 amino acid residues. HbOT1 and HbOT2 were both stable hydrophobic proteins with transmembrane structure and SNARE_assoc domains, possibly belonging to the SNARE_assoc subfamily proteins of the SNARE superfamily. qRT-PCR assays revealed that HbOT1 and HbOT2 were constitutively expressed in different tissues, with HbOT1 highly expressed in roots, stems, barks, and latex, while HbOT2 was highly expressed in latex. In addition, the expressions of HbOT1 and HbOT2 were up-regulated in response to aluminum stress, and they were inducible by metals, such as copper and manganese. Heterologous expression of HbOT1 and HbOT2 in the yeast mutant AD12345678 enhanced the tolerance to oxalic acid and high concentration aluminum stress, which was closely correlated with the secretion of oxalic acid. This study is the first report on oxalate transporter genes in plants, which provides a theoretical reference for the study on the molecular mechanism of oxalic acid secretion to relieve aluminum toxicity and on aluminum-tolerance genetic engineering breeding.

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Section: Discussionmentioning

confidence: 99%

Cloning, Expression Analysis, and Functional Characterization of Candidate Oxalate Transporter Genes of HbOT1 and HbOT2 from Rubber Tree (Hevea brasiliensis)

Yang

Zhao

Peng

et al. 2022

Cells

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“…SNAREs are responsible for mediating vesicle-to-target membrane fusion, and the Arabidopsis genome encodes at least 64 SNAREs (Sanderfoot, 2007;Luo et al, 2022). SNARE proteins are classified as Q-SNARE or R-SNARE according to the core SNARE complex residues (glutamine and arginine, respectively) that contribute to structural assembly.…”

Section: Snares Mediate Membrane Fusion During Cell Plate Formationmentioning

confidence: 99%

“…SNARE proteins are classified as Q-SNARE or R-SNARE according to the core SNARE complex residues (glutamine and arginine, respectively) that contribute to structural assembly. Q-SNAREs are further divided into Qa-, Qb-, Qc-, and Qbc-SNAREs (Fasshauer et al, 1998;Bock et al, 2001;Lipka et al, 2007;Luo et al, 2022). SNAREs are involved in a variety of biological processes such as auxin polar transport, vesicle trafficking, autophagy, gravitropism, and biotic and abiotic stress responses (Saito and Ueda, 2009;Larson et al, 2014;Won and Kim, 2020).…”

Section: Snares Mediate Membrane Fusion During Cell Plate Formationmentioning

confidence: 99%

“…SNAREs are the core machinery mediating membrane fusion, and an important step in membrane fusion is the formation of trans-SNARE complexes, which connect cell membranes so that they can fuse together (Jahn and Scheller, 2006;Saito and Ueda, 2009). Each functional SNARE complex requires two or three Q-SNAREs and one R-SNARE to generate fusion complexes based on homology to synaptic SNAREs; furthermore, cell plate formation requires vesicle fusion mediated by SNAREs and their regulators (Südhof and Rothman, 2009;Luo et al, 2022). Initially, inactive cis-SNARE complexes assemble on the ER and traffic along the secretory pathway through the Golgi and TGN to the cell division plane (Karnahl et al, 2017).…”

Section: Snares Mediate Membrane Fusion During Cell Plate Formationmentioning

confidence: 99%

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Rab GTPases, tethers, and SNAREs work together to regulate Arabidopsis cell plate formation

Shi

Luo²,

Xiang³

et al. 2023

Front. Plant Sci.

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Cell plates are transient structures formed by the fusion of vesicles at the center of the dividing plane; furthermore, these are precursors to new cell walls and are essential for cytokinesis. Cell plate formation requires a highly coordinated process of cytoskeletal rearrangement, vesicle accumulation and fusion, and membrane maturation. Tethering factors have been shown to interact with the Ras superfamily of small GTP binding proteins (Rab GTPases) and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which are essential for cell plate formation during cytokinesis and are fundamental for maintaining normal plant growth and development. In Arabidopsis thaliana, members of the Rab GTPases, tethers, and SNAREs are localized in cell plates, and mutations in the genes encoding these proteins result in typical cytokinesis-defective phenotypes, such as the formation of abnormal cell plates, multinucleated cells, and incomplete cell walls. This review highlights recent findings on vesicle trafficking during cell plate formation mediated by Rab GTPases, tethers, and SNAREs.

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“…Membrane fusion, the last step of vesicular trafficking, is promoted by complex formation of soluble N-ethylmaleimide-sensitive factor attachment receptor proteins (SNAREs) [ 2 ]. SNAREs are located in different subcellular organelles and take part in a series of fundamental processes, for instance, cytokinesis, cytoskeleton organization, symbiosis, growth, and biotic and abiotic stress responses [ 3 , 4 , 5 , 6 ]. For instance, the SYP4 (SYNTAXIN OF PLANTS 4) and SYP6 groups are located in TGN and positively affect tolerance to salinity and osmotic stresses [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the SNARE Family in Cultivated Peanut (Arachis hypogaea L.) Reveals That Some Members Are Involved in Stress Responses

Peng

Liu

et al. 2023

IJMS

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The superfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins mediates membrane fusion during vesicular transport between endosomes and the plasma membrane in eukaryotic cells, playing a vital role in plant development and responses to biotic and abiotic stresses. Peanut (Arachis hypogaea L.) is a major oilseed crop worldwide that produces pods below ground, which is rare in flowering plants. To date, however, there has been no systematic study of SNARE family proteins in peanut. In this study, we identified 129 putative SNARE genes from cultivated peanut (A. hypogaea) and 127 from wild peanut (63 from Arachis duranensis, 64 from Arachis ipaensis). We sorted the encoded proteins into five subgroups (Qa-, Qb-, Qc-, Qb+c- and R-SNARE) based on their phylogenetic relationships with Arabidopsis SNAREs. The genes were unevenly distributed on all 20 chromosomes, exhibiting a high rate of homolog retention from their two ancestors. We identified cis-acting elements associated with development, biotic and abiotic stresses in the promoters of peanut SNARE genes. Transcriptomic data showed that expression of SNARE genes is tissue-specific and stress inducible. We hypothesize that AhVTI13b plays an important role in the storage of lipid proteins, while AhSYP122a, AhSNAP33a and AhVAMP721a might play an important role in development and stress responses. Furthermore, we showed that three AhSNARE genes (AhSYP122a, AhSNAP33a and AhVAMP721) enhance cold and NaCl tolerance in yeast (Saccharomyces cerevisiae), especially AhSNAP33a. This systematic study provides valuable information about the functional characteristics of AhSNARE genes in the development and regulation of abiotic stress responses in peanut.

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SNAREs Regulate Vesicle Trafficking During Root Growth and Development

Cited by 22 publications

References 156 publications

Cloning, Expression Analysis, and Functional Characterization of Candidate Oxalate Transporter Genes of HbOT1 and HbOT2 from Rubber Tree (Hevea brasiliensis)

Cloning, Expression Analysis, and Functional Characterization of Candidate Oxalate Transporter Genes of HbOT1 and HbOT2 from Rubber Tree (Hevea brasiliensis)

Rab GTPases, tethers, and SNAREs work together to regulate Arabidopsis cell plate formation

Genome-Wide Analysis of the SNARE Family in Cultivated Peanut (Arachis hypogaea L.) Reveals That Some Members Are Involved in Stress Responses

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