SNHG8 is identified as a key regulator of epstein-barr virus(EBV)-associated gastric cancer by an integrative analysis of lncRNA and mRNA expression

Huang, Tao; Ji, Yan; Hu, Dan; Chen, Baozheng; Zhang, Hejun; Li, Chao; Chen, Gang; Luο, Xingguang; Xiao, Zheng; Lin, Xiandong

doi:10.18632/oncotarget.13167

Cited by 48 publications

(44 citation statements)

References 89 publications

(99 reference statements)

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“…Previous research indicated that lncRNA SNHG8 is highly expressed in EBV-associated GC [19]. To elucidate the expression levels of SNHG8 in HCC tissues, we found that SNHG8 expression was noticeably increased in HCC tissues (n = 23) as compared with the adjacent normal tissues (n = 23; Fig.…”

Section: Lncrna Snhg8 Expression Was Upregulated In Human Hcc Tissuesmentioning

confidence: 75%

lncRNA SNHG8 Promotes the Tumorigenesis and Metastasis by Sponging miR-149-5p and Predicts Tumor Recurrence in Hepatocellular Carcinoma

Dong

Teng

Guo

et al. 2018

Cell Physiol Biochem

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Background/Aims: Long noncoding RNAs (lncRNAs) are aberrantly expressed in multiple malignant tumors involved in tumor growth and metastasis. Accumulating data show that small nucleolar RNA host gene (SNHG) 1/12/20 plays a key role in the progression of hepatocellular carcinoma (HCC). However, the molecular mechanisms by which SNHG8 contributes to HCC remain elusive and merit exploration. Methods: The association between SNHG8 expression and the clinicopathological characteristics and prognoses in HCC patients was analysed by using qRT-PCR analysis and the data from The Cancer Genome Atlas. Cell growth and metastatic potential were determined by MTT, colony formation, Transwell assays, and the mouse xenograft tumor model and lung metastasis model. Epithelial–mesenchymal transition markers were detected by western blot analysis. The binding capacity of SNHG8 with miRNAs was evidenced by bioinformatic analysis and a luciferase reporter assay. In addition, the rescue experiments were performed based on co-transfection with sh-SNHG8 and a miR-149 inhibitor in HCC cells. Results: The expression levels of lncRNA SNHG8 were dramatically increased in HCC tissues and cell lines as compared with the adjacent normal tissues, and SNHG8 expression was an independent prognostic factor of tumor recurrence in HCC patients. Furthermore, knockdown of SNHG8 inhibited cell proliferation, invasion, and lung metastasis in vitro and in vivo, whereas overexpression of SNHG8 reversed these effects. SNHG8 acted as a sponge of miR-149 and counteracted the tumor suppressive effects of mi R-149 in HCC cells. Expression of phosphatase, Mg2+/Mn2+ dependent 1F, a target of R-149, displayed a negative correlation with miR-149 expression but a positive correlation with SNHG8 expression in HCC specimens. Conclusion: As lncRNA SNHG8 may promote HCC tumorigenesis and metastasis by sponging miR-149, it is a potential candidate marker and therapeutic target for HCC.

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Section: Lncrna Snhg8 Expression Was Upregulated In Human Hcc Tissuesmentioning

confidence: 75%

lncRNA SNHG8 Promotes the Tumorigenesis and Metastasis by Sponging miR-149-5p and Predicts Tumor Recurrence in Hepatocellular Carcinoma

Dong

Teng

Guo

et al. 2018

Cell Physiol Biochem

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“…Small microRNAs (miRNAs) and long noncoding RNAs (IncRNAs) play important post-transcriptional gene regulatory roles in EBVaGC [39, 40]. EBV was the first virus in which miRNAs were discovered [40].…”

Section: Pathophysiology and Tumorigenesismentioning

confidence: 99%

“…IncRNAs are associated with larger tumors, greater invasion and reduced survival rates in gastric cancer [39]. Huang et al showed increased expression of an IncRNA, known as SNHG8 in EBVaGC [39].…”

Section: Pathophysiology and Tumorigenesismentioning

confidence: 99%

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Outlooks on Epstein-Barr virus associated gastric cancer

Naseem

Barzi

Brezden-Masley

et al. 2018

Cancer Treatment Reviews

171

143

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Epstein-Barr virus associated gastric cancer (EBVaGC) comprises approximately 10% of gastric carcinomas. Multiple factors contribute to tumorigenesis, including EBV driven hypermethylation of tumor suppressor genes, inflammatory changes in gastric mucosa, host immune evasion by EBV and changes in cell cycle pathways. The unique molecular characteristics of EBVaGC, such as programmed death ligand 1 (PD-L1) overexpression, highlight the potential for using EBV as a biomarker for response to immunotherapy. Few studies have reported benefit from immunotherapy in EBV positive cancers, and clinical trials investigating the impact of checkpoint inhibitors in EBVaGC are currently underway. This review provides the most recent updates on molecular pathophysiology, epidemiology, clinical features and treatment advances pertaining to EBVaGC.

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“…Long noncoding RNAs (lncRNAs) are over 200 nucleotides in length, lack protein‐coding ability, and retain essential functions in a range of biological processes and collected evidence has confirmed the impact of lncRNAs on cancer initiation, development, and progression . For example, the lncRNA SNHG8 was found to affect several GC‐specific pathways and target genes of EBV . SNHG8 expression was reported to be upregulated 14 fold in GC compared with normal gastric tissues, and in particular, it can increase to still higher levels in EBVaGC than in EBV‐negative GC .…”

Section: Introductionmentioning

confidence: 99%

Differential expression profiling of lncRNAs related to Epstein‐Barr virus infection in the epithelial cells

Zhang

Zuo

et al. 2019

Journal of Medical Virology

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Epstein‐Barr virus (EBV) is a prevalent γ‐herpesvirus associated with a variety of cancers, including epithelial nasopharyngeal carcinoma (NPC) and gastric carcinoma (GC). Long noncoding RNAs (lncRNAs) are emerging as powerful regulators that have demonstrated to play crucial roles in cancer development. In this approach, based on genome‐wide RNA sequencing, we examined the lncRNA expression profiles in four EBV genome‐infected and EBV‐negative 293 cells. A series of lncRNAs were found to be dysregulated in a comparative analysis. Then, eight typical lncRNAs were selected for validation of expression levels by real‐time quantitative polymerase chain reaction and were detected in EBV‐positive or EBV‐negative GC and NPC cells. The differential expression patterns of the eight lncRNAs for validation were fundamentally identical to those revealed in RNA sequencing. Particularly, the differential expression of these lncRNAs in GC and NPC cells indicated their possible roles in EBV infection and tumorigenesis. In addition, a predicted lncRNA‐microRNA‐messenger RNA network suggested their potential interactions. This study reveals the first lncRNAome related to EBV infection in the epithelial cells and provides novel clues for the study of viral role in epithelial cancers through the interaction between EBV and host lncRNAs.

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SNHG8 is identified as a key regulator of epstein-barr virus(EBV)-associated gastric cancer by an integrative analysis of lncRNA and mRNA expression

Cited by 48 publications

References 89 publications

lncRNA SNHG8 Promotes the Tumorigenesis and Metastasis by Sponging miR-149-5p and Predicts Tumor Recurrence in Hepatocellular Carcinoma

lncRNA SNHG8 Promotes the Tumorigenesis and Metastasis by Sponging miR-149-5p and Predicts Tumor Recurrence in Hepatocellular Carcinoma

Outlooks on Epstein-Barr virus associated gastric cancer

Differential expression profiling of lncRNAs related to Epstein‐Barr virus infection in the epithelial cells

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