SOAT1 methylation is associated with coronary heart disease

Abstract

BackgroundThis study was designed to investigate whether differential DNA methylationin of cholesterol absorption candidate genes can function as a biomarker for patients with coronary heart disease (CHD).MethodsDNA methylation levels of the candidate genes FLOT1, FLOT2 and SOAT1 were measured in peripheral blood leukocytes (PBLs) from 99 patients diagnosed with CHD and 89 control subjects without CHD. A total of 110 CPG sites around promoter regions of them were examined.ResultsCompared with groups without CHD, patients with CHD had lower methylation levels of SOAT1 (P<0.001). When each candidate genes were divided into different target segments, patients with CHD also had lower methylation levels of SOAT1 than patients without (P = 0.005). After adjustment of other confounders, methylation levels of SOAT1 were still associated with CHD (P = 0.001, OR = 0.290, 95% CI: 0.150–0.561).ConclusionsSOAT1 methylation may be associated with development of CHD. Patients with lower methylation levels in SOAT1 may have increased risks for CHD. Further studies on the specific mechanisms of this relationship are necessary.

piRNA-6426 increases DNMT3B-mediated SOAT1 methylation and improves heart failure

“…A study reported that the methylation level SOAT1 is lower in patients with heart disease than normal [ 16 ]. We used the online prediction software MethPrimer ( http://www.urogene.org/methprimer ) to predict the CPG islands in SOAT1 promoter region.…”

“…A study showed that the methylation level of SOAT1 in patients with coronary heart disease is significantly decreased [ 16 ]. We found that the overexpression of piRNA-6426 could increase the methylation level of SOAT1 promoter and reduce the expression levels of SOAT1 mRNA and protein, while the interference of piRNA-6426 had the opposite effect.…”

“…Sterol o-acyltransferase 1 (SOAT1) is a key enzyme that converts endoplasmic reticulum cholesterol into cholesterol esters to store lipid droplets [ 14 ]. And it is up-regulated in cardiovascular diseases such as atherosclerosis [ 15 ] and coronary heart disease [ 16 ]. In addition, up-regulation of SOAT1 could further promote the expression level of inflammatory factors [ 17 ].…”

“…In addition, up-regulation of SOAT1 could further promote the expression level of inflammatory factors [ 17 ]. A study found that the methylation level of SOAT1 promoter is lower in patients with coronary heart disease and higher in patients with non-coronary heart disease [ 16 ]. DNA methylation refers to the regulation of gene expression without changing the DNA base sequence mediated by DNA methyltransferases [ 18 ].…”

Roles and Mechanisms of DNA Methylation in Vascular Aging and Related Diseases

“…DNA methylation and hydroxymethylation among the elderly CHD patients were significantly upgraded. Report supported that a lower methylation level in the SOAT1 gene might enhance the risk of CHD ( Abuzhalihan et al, 2019 ).…”