Gender dysphoria (GD) is characterized by a marked discordance between the psychological perception of individual sex and the biological phenotype. In the present article, the authors aimed to analyze the clinical data of a cohort of subjects diagnosed with GD, referred to a national unit, specialized in the endocrine, psychiatric and surgical treatment of this entity. Methods: Data about demographics and response to treatment, were retrospectively analysed in 85 subjects diagnosed with GD, who were observed in the Endocrinology Consultation, during a 12-year period. Results: It was verifi ed that among 85 patients included in the study, 38 (44.7%) were transgender females and 47 (55.3%) were transgender males. The number of patients seeking treatment substantially increased in the last 5 years, with an inferior age of referral in transgender males. Sixty-three patients (74.1%) started cross-sex hormone therapy, deprived of signifi cant adverse events, and gender affi rming surgery was performed in 25 patients (29.4%). Conclusion: Our study revealed a progressively growing number of patients seeking sexual reassignment, being predominantly transgender males. The majority of subjects started hormone therapy without substantial related adverse events, corroborating that it may be considered as a relatively safe treatment. Gender affi rming surgery was performed in a reasonable number of patients, which was comparable with the experience of other centers.