Sodium alginate hydrogel integrated with type III collagen and mesenchymal stem cell to promote endometrium regeneration and fertility restoration

Shuai, Qizhi; Liang, Yuxiang; Xu, Xinrui; Halbiyat, Zulala; Wang, Xiaowan; Cheng, Jingwen; Liu, Jialing; Huang, Tingjuan; Peng, Zhiwei; Wang, Lei; He, Sheng; Zhao, Hong; Liu, Zhizhen; Xu, Jun; Xie, Jun

doi:10.1016/j.ijbiomac.2023.127314

Cited by 12 publications

(8 citation statements)

References 65 publications

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“…The immunofluorescence staining method was conducted according to previously described protocol [14]. Liver organoids were fixed in 4% PFA for 4 hours, dehydrated, embedded in paraffin, and sectioned at 4 μm thickness.…”

Section: Immunofluorescence (If)mentioning

confidence: 99%

Development of alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell derived liver organoids

Feng,

Zhou,

Shuai

et al. 2024

Preprint

Self Cite

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Alcoholic liver disease (ALD) poses a significant health challenge, demanding comprehensive research efforts to enhance our comprehension and treatment strategies. However, the development of effective treatments is hindered by the limitations of existing liver disease models. Liver organoids, characterized by their cellular complexity and three-dimensional (3D) tissue structure closely resembling the human liver, hold promise as ideal models for liver disease research. In this study, we employ a meticulously designed protocol involving the differentiation of human induced pluripotent stem cells (hiPSCs) into liver organoids. This process incorporates a precise combination of cytokines and small molecule compounds within a 3D culture system to guide the differentiation process. Subsequently, these differentiated liver organoids are subjected to ethanol treatment to induce ALD, thus establishing a disease model. Rigorous assessment through a series of experiments reveals that this model partially replicates key pathological features observed in clinical ALD, including cellular mitochondrial damage, elevated cellular reactive oxygen species (ROS) levels, fatty liver, and hepatocyte necrosis. In addition, this model offers potential utility in screening drugs for ALD treatment. Taken together, the liver organoids model of ALD, derived from hiPSCs differentiation, emerges as an invaluable platform for advancing our understanding and management of ALD in clinical settings.

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Section: Immunofluorescence (If)mentioning

confidence: 99%

Development of alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell derived liver organoids

Feng,

Zhou,

Shuai

et al. 2024

Preprint

Self Cite

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“…Endometrial disease refers to many disorders that impact the endometrium, which is the inner lining of the uterus. Endometriosis and endometritis are prevalent and significant disorders (Heil et al, 2023;Shuai et al, 2023). Endometritis is inflammation or irritation of the endometrium.…”

Section: Treatment With Antibiotics and Vmt Slows The Development Of ...mentioning

confidence: 99%

Vaginal microbiota transplantation is a truly opulent and promising edge: fully grasp its potential

Meng,

Sun,

Zhang

2024

Front. Cell. Infect. Microbiol.

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Vaginal microbiota transplantation (VMT) is a cutting-edge treatment modality that has the potential to revolutionize the management of vaginal disorders. The human vagina is a complex and dynamic ecosystem home to a diverse community of microorganisms. These microorganisms play a crucial role in maintaining the health and well-being of the female reproductive system. However, when the balance of this ecosystem is disrupted, it can lead to the development of various vaginal disorders. Conventional treatments, such as antibiotics and antifungal medications, can temporarily relieve the symptoms of vaginal disorders. However, they often fail to address the underlying cause of the problem, which is the disruption of the vaginal microbiota. In recent years, VMT has emerged as a promising therapeutic approach that aims to restore the balance of the vaginal ecosystem. Several studies have demonstrated the safety and efficacy of VMT in treating bacterial vaginosis, recurrent yeast infections, and other vaginal conditions. The procedure has also shown promising results in reducing the risk of sexually transmitted infections and preterm birth in pregnant women. However, more research is needed to establish optimal donor selection, preparation, and screening protocols, as well as long-term safety and efficacy. VMT offers a safe, effective, and minimally invasive treatment option for women with persistent vaginal problems. It could improve the quality of life for millions of women worldwide and become a standard treatment option shortly. With further research and development, it could potentially treat a wide range of other health problems beyond the scope of vaginal disorders.

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“…Immunofluorescence staining was conducted according to a previously described protocol [14]. Liver organoids were fixed in 4% PFA for 4 h, dehydrated, embedded in paraffin, and sectioned at 4-μm thickness.…”

Section: Immunofluorescence (If) Stainingmentioning

confidence: 99%

Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids

Feng,

Zhou,

Shuai

et al. 2024

ABBS

Self Cite

View full text Add to dashboard Cite

Alcoholic liver disease (ALD) poses a significant health challenge, so comprehensive research efforts to improve our understanding and treatment strategies are needed. However, the development of effective treatments is hindered by the limitation of existing liver disease models. Liver organoids, characterized by their cellular complexity and three-dimensional (3D) tissue structure closely resembling the human liver, hold promise as ideal models for liver disease research. In this study, we use a meticulously designed protocol involving the differentiation of human induced pluripotent stem cells (hiPSCs) into liver organoids. This process incorporates a precise combination of cytokines and small molecule compounds within a 3D culture system to guide the differentiation process. Subsequently, these differentiated liver organoids are subject to ethanol treatment to induce ALD, thus establishing a disease model. A rigorous assessment through a series of experiments reveals that this model partially recapitulates key pathological features observed in clinical ALD, including cellular mitochondrial damage, elevated cellular reactive oxygen species (ROS) levels, fatty liver, and hepatocyte necrosis. In addition, this model offers potential use in screening drugs for ALD treatment. Overall, the liver organoid model of ALD, which is derived from hiPSC differentiation, has emerged as an invaluable platform for advancing our understanding and management of ALD in clinical settings.

show abstract

Sodium alginate hydrogel integrated with type III collagen and mesenchymal stem cell to promote endometrium regeneration and fertility restoration

Cited by 12 publications

References 65 publications

Development of alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell derived liver organoids

Development of alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell derived liver organoids

Vaginal microbiota transplantation is a truly opulent and promising edge: fully grasp its potential

Development of an alcoholic liver disease model for drug evaluation from human induced pluripotent stem cell-derived liver organoids

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