Sodium azide mutagenesis within temporary immersion bioreactors modifies sugarcane in vitro micropropagation rates and aldehyde, chlorophyll, carotenoid, and phenolic profiles

Gómez, Daviel; Hernández, Lázaro; Martínez, José Dimas López; Escalante, Doris; Zevallos, Byron E.; Yabor, Lourdes; Trethowan, Richard; Beemster, Gerrit T.S.; Sershen,; Lorenzo, José Carlos

doi:10.1007/s11738-019-2911-0

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…The genotoxicity of NaN 3 has been extensively studied, and its produced metabolite β-azidoalanine fraction [N 3 -CH 2 -CH(NH 2 )-COOH] plays a mutagenic role, inducing chromosomal mutations at a lower rate than other chemical mutagens ( Gomez et al., 2019 ). Thus NaN 3 is characterized by random regions causing a high frequency of mutations and a low frequency of chromosomal abnormalities ( Turkoglu et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Lai

et al. 2023

Front. Plant Sci.

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Plumbago indica L. is a perennial herb with ornamental and anticancer medicinal functions widely distributed in the tropics. It is affected by temperature and cannot bloom normally in colder subtropical regions, which seriously affects its ornamental value. To create low-temperature resistance mutants and enrich new germplasm resources, this study used tissue culture and chemical reagent (0.5 mmol/L NaN3) and low-temperature stress (0°C, full darkness for 48h) induction to target and screen for cold-resistance mutants. The results showed that the ISSR band polymorphism ratio of the 24 suspected mutant materials was 87.5%. The DNA profiles of the 9 mutants initially identified were altered. The content of plumbagin in the stems and leaves of the mutants was examined, and it was found that the accumulation in the leaves of the mutant SA24 could be as high as 3.84 times that of the control, which was 0.5991%. There were significant differences in the anatomical structures of roots, stems and leaves. The mutants mostly exhibited reduced root diameter (only 0.17-0.69 times that of CK), increased stem diameter (up to 2.19 times that of CK), enlarged mesophyll cells, increased thickness (up to 1.83 times that of CK) and high specificity, which are thought to be important for the different cold resistance obtained by the mutants. In the cold resistance experiment, four cold-tolerant mutants were successfully screened according to their morphological characteristics and physiological indexes, and the mutagenesis efficiency could be as high as 2.22% and did not affect the accumulation of plumbagin in their stems and leaves, even higher than CK. The responses of the screened mutants SA15, SA19, SA23 and SA24 to low temperature showed slower leaf wilting, higher light energy conversion efficiency, less accumulation of MDA content, increased enzymatic activities of antioxidant enzymes (SOD, CAT, POD) and more accumulation of soluble sugars and proline content. These characteristics are consistent with the response of cold-resistance plants to low temperatures. The cold- resistance mutants cultivated in soil were observed of agronomic and ornamental traits for one year, mainly manifested as delayed flowering and delayed entry into the senescence stage. This study provides a more rapid and accurate technique for identifying and screening cold-tolerant mutants, and lays the foundation for future experiments on the creation of new cold-resistant varieties.

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Section: Discussionmentioning

confidence: 99%

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Lai

et al. 2023

Front. Plant Sci.

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“…TIS provides the most natural environment for in vitro culture of shoots, somatic embryos, and micro-tubers. It is recognized as a perspective technology for plant micropropagation, production of secondary metabolites ( Pérez-Alonso et al., 2012 ), transgenic plant selection ( Espinosa et al., 2002 ), and evaluation of an organism’s response to abiotic stresses (drought, salinity, flooding, and high temperature) ( Gómez et al., 2019 ). Another perspective for the application of TIS is the mass propagation of plants used in phytoremediation ( Ergönül and Sidal, 2021 ) or the reduction in the inhibitory effects of phenolics by anaerobic digestion ( Kietkwanboot et al., 2022 ).…”

Section: Temporary Immersion Culture: An Efficient Technology For Se ...mentioning

confidence: 99%

Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes

Aguilar

Wang²,

Escalona³

et al. 2022

Front. Plant Sci.

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Culture in temporary immersion systems (TIS) is a valuable tool for the semi-automation of high frequency somatic embryogenesis of coffee. This system allows the intermittent exposure of explants to liquid medium in cycles of specific frequency and duration of immersion with renewal of the culture atmosphere in each cycle. TIS have revolutionized somatic embryogenesis of coffee plants as an alternative for scaling up and reducing costs associated with labor-intensive solid media culture. In Central America, somatic embryogenesis is employed on a commercial scale to produce F1 Coffea arabica hybrids. In Asia and Africa, somatic embryogenesis is used for the multiplication of selected genotypes of C. arabica and C.canephora. Somatic embryogenesis of coffee plants is considered a model system for woody species due to its biological versatility and low frequency of somaclonal variation. Nevertheless, the success of somatic embryogenesis for mass propagation of coffee plants depends on the development, optimization, and transfer of complementary technologies. Temporary immersion using the RITA® bioreactor is, so far, the best complementary tool for somatic embryogenesis of Arabica coffee for a single recipient with simple changes in liquid media. Likewise, high volume bioreactors, such as 10-L glass BIT® and 10-L flexible disposable plastic bags, have been successfully used for somatic embryogenesis of other coffee species. These bioreactors allow the manipulation of thousands of embryos under semi-automated conditions. The protocols, advantages, and benefits of this technology have been well documented for organogenesis and somatic embryogenesis pathways. However, adaptation in commercial laboratories requires technical and logistical adjustments based on the biological response of the cultures as well as the costs of implementation and production. This review presents the historical and present background of TIS and its commercial application and, in particular, pertinent information regarding temporary immersion culture for C. arabica somatic embryogenesis. The main limitations of this technology, such as hyperhydricity, asynchrony, and developmental abnormalities, are examined, and a critical analysis of current knowledge regarding physiological, biochemical, and molecular aspects of the plant response to temporary immersion is offered. Further, perspectives are provided for understanding and solving the morpho-physiological problems associated with temporary immersion culture of coffee plants.Systematic Review Registration:

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Temporary Immersion Bioreactors for Sugarcane Multiplication and Rooting

Spinoso-Castillo,

Serrano-Fuentes,

Sorcia-Morales

et al. 2024

Methods in Molecular Biology

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Sodium azide mutagenesis within temporary immersion bioreactors modifies sugarcane in vitro micropropagation rates and aldehyde, chlorophyll, carotenoid, and phenolic profiles

Cited by 4 publications

References 47 publications

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes

Temporary Immersion Bioreactors for Sugarcane Multiplication and Rooting

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