Sodium pheophorbide a controls cherry tomato gray mold (Botrytis cinerea) by destroying fungal cell structure and enhancing disease resistance-related enzyme activities in fruit

Ji, Jing-Yu; Yang, Jing; Zhang, Bowen; Wang, Shuren; Zhang, Guocai; Lin, Lian-Nan

doi:10.1016/j.pestbp.2020.104581

Cited by 26 publications

(7 citation statements)

References 47 publications

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“…solani, leading to leakage of the nucleic acids, proteins, and electrolytes. Similar results have been observed in Botrytis cinerea ( Ji et al, 2020 ) and Penicillium roqueforti ( Ju et al, 2020 ). After confirming that the membrane permeability of F .…”

Section: Resultssupporting

confidence: 88%

Bioassay-guided isolation of three new alkaloids from Suillus bovinus and preliminary mechanism against ginseng root rot

Xiong,

Yang,

Yao

et al. 2024

Front. Microbiol.

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In order to control the occurrence of ginseng root rot caused by Fusarium solani (Mart.) Sacc., the antifungal compounds of the mushroom Suillus bovinus were investigated. And three new alkaloids (1–3), named bovinalkaloid A–C, along with one known analog (4), were isolated and identified by bioassay-guided isolation and spectroscopic analyses. Compound 1 strongly inhibited the mycelial growth and spore germination of F. solani with minimum inhibitory concentration of 2.08 mM. Increases in electrical conductivity, nucleic acid, and protein contents, and decreases in lipid content showed that the membrane permeability and integrity were damaged by compound 1. Compound 1 also increased the contents of malondialdehyde and hydrogen peroxide and the activities of antioxidant enzymes, indicating that lipid peroxidation had taken place in F. solani. Compound 1 may serve as a natural alternative to synthetic fungicides for the control of ginseng root rot.

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Section: Resultssupporting

confidence: 88%

Bioassay-guided isolation of three new alkaloids from Suillus bovinus and preliminary mechanism against ginseng root rot

Xiong,

Yang,

Yao

et al. 2024

Front. Microbiol.

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“…mongolica. In a study of the inhibitory effect of SPA on gray mold of cherry tomato, Ji et al [23] found that SPA significantly enhanced the activities of SOD, POD, and CAT in the cherry tomatoes to protect plant cells from oxidative damage, consistent with this study. This indicates that SPA can induce resistance to pathogenic fungi by enhancing the activities of protective enzymes in plants, thus reducing the disease incidence.…”

Section: Discussionsupporting

confidence: 89%

“…Sodium pheophorbide a (SPA) is a water-soluble sodium salt of pheophorbide a that is easy to use. This compound can effectively inhibit the growth of fungal pathogens of plant hosts, including the fungal diseases of economically important horticultural crops, such as cherry tomato gray mold caused by gray mold (Botrytis cinerea) [23]. SPA exerts antifungal activities by altering the hyphal morphology, reducing the cell wall integrity, increasing the permeability of the cell membrane, and increasing the activity of defense system-related enzymes in cherry tomatoes.…”

Section: Introduction Species Of Treementioning

confidence: 99%

TMT-Based Proteomic Analysis Reveals the Molecular Mechanisms of Sodium Pheophorbide A against Black Spot Needle Blight Caused by Pestalotiopsis neglecta in Pinus sylvestris var. mongolica

Zhang,

Yang,

Wang

et al. 2024

JoF

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Black spot needle blight is a minor disease in Mongolian Scots pine (Pinus sylvestris var. mongolica) caused by Pestalotiopsis neglecta, but it can cause economic losses in severe cases. Sodium pheophorbide a (SPA), an intermediate product of the chlorophyll metabolism pathway, is a compound with photoactivated antifungal activity, which has been previously shown to inhibit the growth of P. neglecta. In this study, SPA significantly reduced the incidence and disease index and enhanced the chlorophyll content and antioxidant enzyme activities of P. sylvestris var. mongolica. To further study the molecular mechanism of the inhibition, we conducted a comparative proteomic analysis of P. neglecta mycelia with and without SPA treatment. The cellular proteins were obtained from P. neglecta mycelial samples and subjected to a tandem mass tag (TMT)-labelling LC-MS/MS analysis. Based on the results of de novo transcriptome assembly, 613 differentially expressed proteins (DEPs) (p < 0.05) were identified, of which 360 were upregulated and 253 downregulated. The 527 annotated DEPs were classified into 50 functional groups according to Gene Ontology and linked to 256 different pathways using the Kyoto Encyclopedia of Genes and Genomes database as a reference. A joint analysis of the transcriptome and proteomics results showed that the top three pathways were Amino acid metabolism, Carbohydrate metabolism, and Lipid metabolism. These results provide new viewpoints into the molecular mechanism of the inhibition of P. neglecta by SPA at the protein level and a theoretical basis for evaluating SPA as an antifungal agent to protect forests.

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“…It is possible that in the mats grown in hot spring environments, a higher amount of chlorophyll degradation products was present compared to biomasses from laboratory-grown isolates. Phaeophytin a, as well as phaeophorbide a, has been shown to have antimicrobial activity [48,49]. Accumulation of cell degradation products in mats and biofilms might also have led to some fatty acids or their derivatives accumulation compared to laboratoryobtained biomasses.…”

Section: Discussionmentioning

confidence: 99%

Hot Springs Cyanobacteria Endowed with Biological Activities for Cosmetic Applications: Evaluation of On-Site Collected Communities and Isolated Strains

et al. 2023

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Microbial communities growing around hot springs (bioglea), of which cyanobacteria are the main components, contribute to beneficial properties of thermal muds and often enter cosmetic treatment products. Bioglea is obtainable via alternative approaches to harvesting from nature: direct cultivation of whole communities and isolation and cultivation of the isolated strains. Cultivation represents a step towards industrial production, guaranteeing higher availability, quality and safety. In this work, the biochemical composition of natural and cultivated bioglea collected in different hot springs and of cyanobacterial strains isolated thereof was analysed. Lipophilic and hydrophilic extracts were tested for antimicrobial and radical scavenging activities and toxicity against Artemia salina. Higher antimicrobial activity was found in lipophilic than hydrophilic bioglea extracts and the opposite in isolates’ extracts. Thermal water extracts also showed some activity. No toxicity was observed. Bioglea radical scavenging activity positively correlated with carotenoids (lipophilic extracts) and phycobiliproteins (hydrophilic extracts). No correlation was observed for isolated strains. Cultivation at the 10 L scale of Chroococcidiopsis BIOG3 evidenced bioactivity changes with the growth phase and life cycle stage. From activity comparison, communities showed higher potential over single strains cultivation; however, further investigations should elucidate isolates’ full potential also by combining them into synthetic communities to simplify production and standardisation.

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Sodium pheophorbide a controls cherry tomato gray mold (Botrytis cinerea) by destroying fungal cell structure and enhancing disease resistance-related enzyme activities in fruit

Cited by 26 publications

References 47 publications

Bioassay-guided isolation of three new alkaloids from Suillus bovinus and preliminary mechanism against ginseng root rot

Bioassay-guided isolation of three new alkaloids from Suillus bovinus and preliminary mechanism against ginseng root rot

TMT-Based Proteomic Analysis Reveals the Molecular Mechanisms of Sodium Pheophorbide A against Black Spot Needle Blight Caused by Pestalotiopsis neglecta in Pinus sylvestris var. mongolica

Hot Springs Cyanobacteria Endowed with Biological Activities for Cosmetic Applications: Evaluation of On-Site Collected Communities and Isolated Strains

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