Soft tissue integration versus early biofilm formation on different dental implant materials

Zhao, Bingran; Mei, Henny C. van der; Subbiahdoss, Guruprakash; Vries, J. de; Rustema-Abbing, Minie; Kuijer, Roel; Busscher, Henk J.; Ren, Yijin

doi:10.1016/j.dental.2014.04.001

Cited by 160 publications

(188 citation statements)

References 29 publications

Supporting

Mentioning

185

Contrasting

Order By: Relevance

“…A cell-bacteria co-culture study was also performed because it provides a better mimic of the clinical situation than mono-cultures studies with either bacteria or cells [4,17]. The outcome of co-culture studies involving bacteria and tissue cells depends on a multitude of factors [4,5].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

View full text Add to dashboard Cite

Bacterial infection in dental implants along with osseointegration failure usually leads to loss of the device. Bioactive molecules with antibacterial properties can be attached to titanium surfaces with anchoring molecules such as silanes, preventing biofilm formation and improving osseointegration. Properties of silanes as molecular binders have been thoroughly studied, but research on the biological effects of these coatings is scarce. The aim of the present study was to determine the in vitro cell response and antibacterial effects of triethoxysilypropyl succinic anhydride (TESPSA) silane anchored on titanium surfaces. X-ray photoelectron spectroscopy confirmed a successful silanization. The silanized surfaces showed no cytotoxic effects. Gene expression analyses of Sarcoma Osteogenic (SaOS-2) osteoblast-like cells cultured on TESPSA silanized surfaces reported a remarkable increase of biochemical markers related to induction of osteoblastic cell differentiation. A manifest decrease of bacterial adhesion and biofilm formation at early stages was observed on treated substrates, while favoring cell adhesion and spreading in bacteria-cell co-cultures.Surfaces treated with TESPSA could enhance a biological sealing on implant surfaces against bacteria colonization of underlying tissues. Furthermore, it can be an effective anchoring platform of biomolecules on titanium surfaces with improved osteoblastic differentiation and antibacterial properties.

show abstract

Section: Discussionmentioning

confidence: 99%

“…This protocol was modified from a published study [17]. Treated surfaces and control samples were immersed in S.sanguinis and L.salivarius strain suspensions (concentration of 10 8 bacteria ml -1 ) in their corresponding medium and incubated at 37ºC for 2 hours.…”

Section: Cell-bacteria Co-culturesmentioning

confidence: 99%

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Godoy-Gallardo

Guillem-Marti

Sevilla

et al. 2016

Materials Science and Engineering: C

View full text Add to dashboard Cite

show abstract

“…The data indicated that the addition of SNPs significantly increased the titanium surface roughness and decreased its wettability, leading to an increase in CA hysteresis. The impact of the SNPs on the surface roughness after SNP deposition was described by Mei et al, 28 Cabal et al, 16 and Ewald et al 22 A decrease in surface roughness was described by Nakajo et al 14 No differences were noticed by Zhao et al 29 and Li et al 30 An increase in surface wettability associated with nanosilver incorporation was described by Mei et al 28 and Unosson et al, 13 although Gao et al did not observe significant changes in surface wettability after silver incorporation. 20 In the present study, the silver formed clusters resembling microparticulate silver, and this probably increased the surface hydrophobicity, as in the study by …”

Section: Discussionmentioning

confidence: 99%

“…24 Zhao et al suggested that a certain nanotopography could help to prevent excessive ion leakage. 29 In contrast, Zhang et al proposed that the level of hydrophobicity protected the material from excessive silver release. 53 Nevertheless, no reliable technique for Ag + release stabilization has been proposed so far.…”

mentioning

confidence: 99%

In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery

Pokrowiecki¹,

Zaręba²,

Szaraniec³

et al. 2017

IJN

View full text Add to dashboard Cite

The addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. For this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. Titanium disks were incorporated with silver nanoparticles over different time periods by Tollens reaction, which is considered to be an eco-friendly, cheap, and easy-to-perform method. The surface roughness, wettability, and silver release profile of each disc were measured. In addition, the antibacterial activity was also evaluated by using disk diffusion tests for bacteria frequently isolated from the peri-implant biofilm: Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus sanguis, Porphyromonas gingivalis, Staphylococcus aureus , and Escherichia coli . Cytotoxicity was evaluated in vitro in a natural human osteoblasts cell culture. The addition of nanosilver significantly increased the surface roughness and decreased the wettability in a dose-dependent manner. These surfaces were significantly toxic to all the tested bacteria following a 48-hour exposure, regardless of silver doping duration. A concentration of 0.05 ppm was sufficient to inhibit Gram-positive and Gram-negative species, with the latter being significantly more susceptible to silver ions. However, after the exposure of human osteoblasts to 0.1 ppm of silver ions, a significant decrease in cell viability was observed by using ToxiLight™ BioAssay Kit after 72 hours. Data from the present study indicated that the incorporation of nanosilver may influence the surface properties that are important in the implant healing process. The presence of nanosilver on the titanium provides an antibacterial activity related to the bacteria involved in peri-implantitis. Finally, the potential toxicological considerations of nanosilver should further be investigated, as both the antibacterial and cytotoxic properties may be observed at similar concentration ranges.

show abstract

“…Future research on dental implant surfaces with reduced bacterial adhesion must employ validated experiments, preferably multicultural testing or co-culturing 183) under dynamic growth conditions and subsequently backed up by validated in vivo studies.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial dental implant functionalization strategies —A systematic review

Grischke

Eberhard

Stiesch

2016

Dental Materials Journal

View full text Add to dashboard Cite

Soft tissue integration versus early biofilm formation on different dental implant materials

Cited by 160 publications

References 29 publications

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

Anhydride-functional silane immobilized onto titanium surfaces induces osteoblast cell differentiation and reduces bacterial adhesion and biofilm formation

In vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery

Antimicrobial dental implant functionalization strategies —A systematic review

Contact Info

Product

Resources

About