Software for Matching Standard Activity Enzyme Biosensors for Soil Pollution Analysis

Kratasyuk, Valentina A.; Kolosova, Е. М.; Sutormin, Oleg S.; Lonshakova-Mukina, Victoria I.; Baygin, Matvey M.; Rimatskaya, Nadezhda V.; Sukovataya, I. E.; Shpedt, A. A.

doi:10.3390/s21031017

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…Our research group previously showed that the accuracy of the bioluminescence assay, developed for and applied in soil toxicity assessments, is related not only to the presence of a toxicant but also to the humic organic matter content [11][12][13][14]. For instance, humic substances at levels higher than 1 mg/L have a negative impact on the substrate concentration in the bioluminescent reaction, which leads to a decrease in the activity of the enzyme system.…”

Section: Discussionmentioning

confidence: 99%

“…The residual luminescence was calculated according to the formula (I/I 0 ) × 100%. To determine the impact of the analyzed soil sample, the determination scale described earlier [11,12] was used as follows: at I/I 0 > 80%, the analyzed soil sample was considered to have no impact, at 50% < I/I 0 < 80% the analyzed soil sample was considered as having an impact, and at I/I 0 < 50%, the analyzed soil sample was considered to have a significant impact.…”

Section: Bioluminescent Enzymatic Assaymentioning

confidence: 99%

“…In addition, enzymes can be used as a biological part of enzyme-based biosensors. Previously, our research group demonstrated the applicability of a bioluminescent enzyme system for the assessment of heavy metal and pesticide levels in soils [3,[11][12][13][14]. The bacterial coupled enzyme system consists of two enzymes, namely, NAD(P)H:FMN-oxidoreductase and luciferase (Red + Luc), catalyzing the following reactions (1 and 2): NAD(P)Н:FMN -oxidoreductase (Red)…”

Section: Introductionmentioning

confidence: 99%

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Bioluminescent-Inhibition-Based Biosensor for Full-Profile Soil Contamination Assessment

et al. 2022

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A bioluminescent-enzyme-inhibition-based assay was applied to predict the potential toxicity of the full profile of the following soil samples: agricultural grassland, 10-year fallow land (treated with remediation processes for 10 years) and uncontaminated (virgin) land. This assay specifically detects the influence of aqueous soil extracts from soils on the activity of a coupled enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc). It was shown that the inhibitory effect of the full-profile soil samples on the Red + Luc system decreased with depth for the 10-year fallow-land and virgin-land samples, which correlated with a decrease in the humic organic matter content in the soils. The inhibitory effect of the agricultural grassland on the Red + Luc enzyme system activity was more complex and involved the presence of the humic organic matter content, as well as the presence of pollutants in the whole-soil profile. However, if the interfering effect of humic organic substances on the Red + Luc system’s activity is taken into account during full-profile soil toxicity assessments, it might help to detect pollutant mobility and its leaching into the subsoil layer. Thus, this bioluminescent method, due to the technical simplicity, rapid response time and high sensitivity, has the potential to be developed as a biological part of the inhibition-based assay and/or biosensors for the preventive tracing of potential full-profile soil contamination.

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Section: Discussionmentioning

confidence: 99%

Section: Bioluminescent Enzymatic Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioluminescent-Inhibition-Based Biosensor for Full-Profile Soil Contamination Assessment

et al. 2022

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“…The biosensors employ a biological recognition element (bioreceptor), can be a whole cell-base system or cell partbased system, for the detection of toxic pollutants, these devices allow onside quantification and avoid the requirement of sophisticated equipment [31]. The development of a wide type of biosensors using different bioreceptors such as microbial fuel cells [18,[32][33][34][35][36], microorganisms (Whole cell-based) [37][38][39][40], antibodies [41][42][43], enzymes [44][45][46][47][48][49][50], among others has been reported in the literature. On the other hand, biosensors can be placed as a favorable alternative due to the variability of pollutants (Heavy metals, organic compounds, drugs, and microorganisms) that are capable of pollutant trace detection [29].…”

Section: Introductionmentioning

confidence: 99%

Immobilized Enzyme-based Novel Biosensing System for Recognition of Toxic Elements in the Aqueous Environment

et al. 2023

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Access to secure water sources has become one of the biggest challenges for human sustainability. Climate change and associated droughts make it difficult to guarantee the usual water source and move to groundwater use or to the re-use of treated wastewater remains unviable due the lack on the capacity of monitoring water quality. Moreover, reusing treated wastewater from repositories near anthropogenic sources represents a risk of high concentrations of emerging contaminants. The strategies involve a higher risk of encountering toxic elements with a heavy burden on human and environmental health. New accessible and reliable tools are required to detect any hazard from the waterbodies in real time to ensure safe management and also to decrease mismanagement or ilegal water discharges. One of the available options is to look into enzyme-based biosensors that can detect toxic elements in the water. The proposed biosensors require sensible elements to be accessible and durable for their proper function. The present revision shows in first place, the actual need of real time monitoring due the different sources and effects of emergent pollutants. Secondly, describes how enzymes can be immobilized for its application in biosensors and the rol enzymes play as bioreceptor element in biosensing. Thirdly, describes the transduction methods that can be observed, and finally the actual application of enzyme biosensors for the detection of different toxic elements. According to the presented literature enzyme-based biosensors have been successfully applied for the detection of a wide number of pollutants reaching detection limits comparable to traditional methods such as up to 0.018 nM of mercury. Furthermore, laccase seems to be the more applied enzyme in literature, but positive results are not limited to this enzyme and other candidates have been explored showing good detection rate.

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“…The authors of the cited papers chose performance for one inhibitor, but the devices are sensitive to multiple inhibitors. Compared to AChE, the biosensors based on BChE have not gained broader interest through their applicability for practical analyses [ 17 – 20 ]. In this paper, a colorimetric biosensor based on BChE as a recognition element was intended as an analytical tool suitable for the detection of irreversible (organophosphorus) and pseudoirreversible (carbamate) inhibitors of cholinesterase.…”

Section: Introductionmentioning

confidence: 99%

A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay

Pohanka

Zakova

2022

International Journal of Analytical Chemistry

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Biosensors containing cholinesterase are analytical devices suitable for the assay of neurotoxic compounds. In the research on biosensors, a new platform has appeared some years ago. It is the digital photography and scoring of coloration (photogrammetry). In this paper, a colorimetric biosensor is constructed using 3D-printed multiwell pads treated with indoxylacetate as a chromogenic substrate and gold nanoparticles with the immobilized enzyme butyrylcholinesterase. A smartphone camera served for photogrammetry. The biosensor was tested for the assay of carbofuran and paraoxon ethyl as two types of covalently binding inhibitors: irreversible and pseudoirreversible. The biosensor exerted good sensitivity to the inhibitors and was able to detect carbofuran with a limit of detection for carbofuran 7.7 nmol/l and 17.6 nmol/l for paraoxon ethyl. A sample sized 25 μl was suitable for the assay lasting approximately 70 minutes. Up to 121 samples can be measured contemporary using one multiwell pad. The received data fully correlated with the standard spectrophotometry. The colorimetric biosensor exerts promising specifications and appears to be competitive to the other analytical procedures working on the principle of cholinesterase inhibition. Low-cost, simple, and portable design represent an advantage of the assay of the biosensor. Despite the overall simplicity, the biosensor can fully replace the standard spectroscopic methods.

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Software for Matching Standard Activity Enzyme Biosensors for Soil Pollution Analysis

Cited by 7 publications

References 17 publications

Bioluminescent-Inhibition-Based Biosensor for Full-Profile Soil Contamination Assessment

Bioluminescent-Inhibition-Based Biosensor for Full-Profile Soil Contamination Assessment

Immobilized Enzyme-based Novel Biosensing System for Recognition of Toxic Elements in the Aqueous Environment

A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay

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