“… 40 , 41 In contrast, open-source software, including CellProfiler, allows image analysis methods and codes not only to be accessible to investigators but also to be interrogated, extended, enhanced, and shared by a wider community, which can solve the majority of the challenges around data management, visualization, and interactivity while permitting others to add new algorithms and functionality for specific tasks. 39 , 42 , 43 CellProfiler is an image analysis software released in 2005 and has since undergone a series of improvements to become one of the most widely used forms of software for pathomics analyses due to its versatility, continuous improvements, and ease of use. 24 , 43 Although some useful features might be ignored by using the inherent software extraction method of CellProfiler, there is no perfect software or method providing features that support all studies; thus, a more suitable software or method can be selected for individual research needs.…”