Soil bio-solarization and Trichoderma asperellum suppress black root rot disease and increase strawberry yield

Abstract

Trichoderma asperellum applied as pellets, disks, or spore suspension against the causal agents of strawberry-black root rot disease represented by three fungal species was tested. The pellets/disks could significantly reduce the growth area of the pathogenic fungi Rhizoctonia solani, Fusarium solani, and Macrophomina phaseolina in vitro. Solarization via mulching soil with transparent polyethylene sheets could affect these pathogens. It reduced the counts of the fungi buried in cloth bags at 1–10 cm of soil surface by 70, 65, and 65% for R. solani, F. solani, and M. phaseolina, respectively relative to unmulched soil. Yet, the more depths of bags in soil, the less the mulching affects the three pathogens. In two field experiments, the disease incidence and severity were more reduced when T. asperellum pellets/suspension was integrated with bio-solarization (mulching soon after bio-fumigation with Al-Abour Compost®)) than any single treatment. The highest reductions obtained by T. asperellum pellets combined with bio-solarization averaged 75 and 73.8%, respectively. Bio-solarization with Actamyl affected the disease incidence/severity to a lesser extent. All tested treatments significantly boosted yield of strawberry plants. Strawberry yield and activities of the pathogenesis-related proteins peroxidase and chitinase showed favorable responses almost parallel to the extent of reduction in the disease incidence and severity caused by all treatments. The highest yield increase achieved via combining T. asperellum pellets with bio-solarization was 160.8%. Our results support hypothetical biocontrol potential that T. asperellum and bio-solarization together with adequate phytosanitary measures can reliably control strawberry-black root rot disease and enhance strawberry yield.