Soil carbonate decomposition by acid has little effect on δ13C of organic matter

Midwood, Andrew J.; Boutton, Thomas W.

doi:10.1016/s0038-0717(98)00030-3

Cited by 255 publications

(118 citation statements)

References 21 publications

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“…The soil samples that passed through the sieve were air-dried and ground (< 0.15 mm). For 13 C measurement in soil organic C (SOC), carbonates were removed from the soil samples by washing in 0.1 M HCl for 24 h (Midwood and Boutton, 1998), the samples were then neutralized by adding deionized water and dried at 40 • before the 13 C measurement. The roots were carefully washed with river water and rinsed with deionized water through a 0.15 mm screen to remove attached soil and dark-brown/black debris.…”

Section: Samplingmentioning

confidence: 99%

Field 13CO2 pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a Kobresia meadow

Zou

Zhao

et al. 2014

Biogeosciences

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Abstract. Livestock exclosure has been widely used as an approach for grassland restoration. However, the effects of exclosures on grasslands are controversial and can depend on many factors, such as the grassland ecosystem types, evolutionary history and so on. In this study, we conduct field experiments to investigate the variations of the ecosystem function in response to livestock exclosure in a Kobresia humilis meadow with 6 years of grazing exclosure on the QinghaiTibetan Plateau. We focused on two ecosystem functions: plant community structure and ecosystem carbon cycling. The plant aboveground productivity, plant diversity and the composition of plant functional groups of the meadow were addressed as the indicators of the plant community structure. The 13 C isotope pulse labeling technique was applied to evaluate the alterations of ecosystem carbon cycling during a short term. The results showed that the plant community structure was changed after being fenced in for 6 years, with significantly decreased aboveground productivity, species loss and varied composition of the four plant functional groups (grasses, sedges, legumes and forbs). Using the pulse labeling technique, we found a lower cycling rate of 13 C in the plant-soil system of the fenced plots compared with the grazed sites during the first 24 h after labeling. A higher proportion of recovered 13 C in the plant-soil system migrated into the soil as root exudates immediately after labeling at both fenced and control grazed sites, with a significantly lower proportion in the fenced site, coinciding with the lower proportion of 13 C lost from soil respiration. Thirtytwo days after labeling, 37 % of the recovered 13 C remained in the soil of the fenced plots, with significant differences compared to in the grazed plots (47 %). In addition, less 13 C (5 vs. 7 %) was lost by soil respiration in the fenced plots during the chase period of 32 days. Overall, our study suggests that livestock exclosures have negative effects on the plant community structure and partitioning patterns of the photoassimilated carbon in the Kobresia meadow, and the effects on photoassimilated carbon cycling are likely to result from the variations of community structures in the ecosystem.

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Section: Samplingmentioning

confidence: 99%

Field 13CO2 pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a Kobresia meadow

Zou

Zhao

et al. 2014

Biogeosciences

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“…HCl vap is based on Hedges and Stern (1984). HCl aq was carried out using 1N HCl to avoid inefficient carbonate removal that has been reported for dilute (0.1 N) solutions, and potential alteration of δ 13 C OC that has been reported for concentrated (6 N) solutions (Midwood and Boutton 1998). Bosley and Wainright (1999) found no change in δ 13 C signatures of two species of marine animals when treated with 1N HCl.…”

Section: Materials and Proceduresmentioning

confidence: 99%

Carbonate removal from coastal sediments for the determination of organic carbon and its isotopic signatures, δ¹³C and Δ¹⁴C: comparison of fumigation and direct acidification by hydrochloric acid

Komada

Anderson

Dorfmeier

2008

Limnology & Ocean Methods

175

106

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“…The occluded light fraction was also separated by density floatation in NaI (density 51.8 g cm (3 ) but after mechanical dispersion of the soil by sonication. Prior to extraction of humic substances, the HF was pre-treated with 0.5 M HCl to remove carbonates (Midwood and Boutton 1998). Carbonates in whole soil samples were also removed using the same procedure.…”

Section: Extraction Of Soil Humic Substancesmentioning

confidence: 99%

Distribution of organic carbon in the stable soil humic fractions as affected by tillage management

Murage¹,

Voroney²

2008

Can. J. Soil. Sci.

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Murage, E. W. and Voroney, P. 2008. Distribution of organic carbon in the stable soil humic fractions as affected by tillage management. Can. J. Soil Sci. 88: 99Á106. Soil humus comprises a large and stable pool of soil organic matter (SOM); hence a better understanding of the fate of C in soil humic fractions can provide valuable information for the development of alternative tillage practices that will lead to long-term soil C sequestration. We used d 13 C techniques to investigate the effects of tillage on the dynamics of native (C 3 ÃC) and corn derived C (C 4 ÃC) in fulvic acid (FA), humic acid (HA) and humin fractions. Humic substances were extracted from soils cropped to corn for 11 yr and managed under either conventional (CT) or no-tillage (NT), and from a conventionally tilled soil under 55 yr of tobacco/rye rotation. Notillage resulted in higher proportions of C 4 ÃC in the upper 5 cm and generally lower C 4 ÃC proportions below 5 cm than CT. Up to 31, 27 and 34% of C 4 ÃC were assimilated into FA, HA and humin fractions, respectively, indicating that even the humin fraction, often described as passive, old or resistant, acted as a sink of recently added C, and that it is heterogeneous with some of its components being young. Recovery of large proportions of C 3 ÃC in the humic fractions demonstrated their importance in the long-term stabilization of SOM. Within each sampling depth, there were no unique differences in the distribution of C 3 ÃC among the three humic fractions, suggesting similar turnover of C 3 ÃC in all the fractions. Therefore, there was no unique active fraction corresponding with the concept of C pools with defined turnover characteristics used in models of SOM turnover.Key words: Soil humic fractions, corn derived C, native C, d13 C techniques, tillage practicesMurage, E. W. et Voroney, P. 2008. Incidence du travail du sol sur la re´partition du carbone organique dans les fractions humiques stables. Can. J. Soil Sci. 88: 99Á106. L'humus du sol constitue un important et stable re´servoir de matie`re organique. C'est pourquoi mieux comprendre le sort du carbone dans les fractions humiques nous procurerait des renseignements pre´cieux pour e´laborer de nouvelles pratiques de travail du sol susceptibles de favoriser une se´questration al ong terme du carbone. Les auteurs se sont servis des techniques au d 13 C pour e´tudier les conse´quences du travail du sol sur la dynamique du carbone naturel (C ÃC 3 ) et du carbone issu du maı¨s (CÃC 4 ) dans l'acide fulvique (AF), l'acide humique (AH) et l'humine. Les substances humiques ont e´te´extraites d'un sol sur lequel on avait cultive´du maı¨s pendant 11 ans avec travail classique (TC) et non-travail du sol (NT), ainsi que d'un sol travaille´de la manie`re usuelle sur lequel du tabac et du seigle avaient e´te´cultive´s en rotation pendant plus de 55 ans. Le non-travail du sol engendre une plus forte proportion de C ÃC 4 que le TC a`une profondeur supe´rieure a`5 cm. Jusqu'a`31, 27 et 34 % du CÃC 4 se transforme respectivement en AF, AH et...

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Soil carbonate decomposition by acid has little effect on δ13C of organic matter

Cited by 255 publications

References 21 publications

Field <sup>13</sup>CO<sub>2</sub> pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a <i>Kobresia</i> meadow

Field <sup>13</sup>CO<sub>2</sub> pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a <i>Kobresia</i> meadow

Carbonate removal from coastal sediments for the determination of organic carbon and its isotopic signatures, δ¹³C and Δ¹⁴C: comparison of fumigation and direct acidification by hydrochloric acid

Distribution of organic carbon in the stable soil humic fractions as affected by tillage management

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Soil carbonate decomposition by acid has little effect on δ13C of organic matter

Cited by 255 publications

References 21 publications

Field &lt;sup&gt;13&lt;/sup&gt;CO&lt;sub&gt;2&lt;/sub&gt; pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a &lt;i&gt;Kobresia&lt;/i&gt; meadow

Field &lt;sup&gt;13&lt;/sup&gt;CO&lt;sub&gt;2&lt;/sub&gt; pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a &lt;i&gt;Kobresia&lt;/i&gt; meadow

Carbonate removal from coastal sediments for the determination of organic carbon and its isotopic signatures, δ13C and Δ14C: comparison of fumigation and direct acidification by hydrochloric acid

Distribution of organic carbon in the stable soil humic fractions as affected by tillage management

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Field <sup>13</sup>CO<sub>2</sub> pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a <i>Kobresia</i> meadow

Field <sup>13</sup>CO<sub>2</sub> pulse labeling reveals differential partitioning patterns of photoassimilated carbon in response to livestock exclosure in a <i>Kobresia</i> meadow

Carbonate removal from coastal sediments for the determination of organic carbon and its isotopic signatures, δ¹³C and Δ¹⁴C: comparison of fumigation and direct acidification by hydrochloric acid