Soil Contamination with Heavy Metals and Petroleum Derivates: Impact on Edaphic Fauna and Remediation Strategies

Souza, Rogéria Cristiane Gratão de; Maziviero, Thiago Guilherme; Christofoletti, Cintya Aparecida; Pinheiro, Tamaris Gimenez; Fontanetti, Carmem Silvia

doi:10.5772/52868

Cited by 12 publications

(8 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Soil conservation is necessary since many important life-related processes take place in this ecosystem, including food production, biogeochemical cycles, industrial uses, and mineral extraction, besides being the substrate of our living environment. Regrettably, these human activities are the main source of soil pollution [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Laboratory-Scale Biodegradation of Fuel Oil No. 6 in Contaminated Soils by Autochthonous Bacteria

Piñón-Castillo¹,

Gutiérrez²,

Serna³

et al. 2018

Advances in Bioremediation and Phytoremediation

View full text Add to dashboard Cite

In order to evaluate the degradation of fuel oil no. 6 (FO6) in contaminated soil, laboratory-scale bioreactors were set up to study biostimulation, bioaugmentation, and natural attenuation processes. A solution of fertilizers was added in biostimulation and biouagmentation (0.03% N, 0.01% P). To the bioaugmentation process, an enrichment culture of indigenous hydrocarbon-degrading microorganisms was also added once a week. Total aerobic and hydrocarbon-degrading microorganisms were determined by plate count, and total petroleum hydrocarbon (TPH) concentration was determined gravimetrically (EPA method 9071b) every 15 days. After 1 year of study, degradation rate was higher for biostimulation (0.19 g TPH/day), followed by natural attenuation (0.18 g TPH/day) and bioaugmentation (0.16 g TPH/day). TPH showed a change in composition of hydrocarbons, attributed to microbiological activity. Microbial counts of hydrocarbon-degrading microorganisms were on the range of 4-6 log CFU/g soil. Preliminary bacterial identification corresponded to Pseudomonas, Rhodococcus, Actinomyces, and Bacillus strains; randomly amplified polymorphic DNA (RAPD); and denaturing gradient gel electrophoresis (DGGE) analysis demonstrated a large microbial diversity. From the degradation rates, it can be predicted that such limits will be achieved by increasing further 107-117 days of the treatments. Results demonstrated to be efficient on the restoration of contaminated soil, being an alternative to treat soils contaminated with heavy hydrocarbons.

show abstract

Section: Introductionmentioning

confidence: 99%

Laboratory-Scale Biodegradation of Fuel Oil No. 6 in Contaminated Soils by Autochthonous Bacteria

Piñón-Castillo¹,

Gutiérrez²,

Serna³

et al. 2018

Advances in Bioremediation and Phytoremediation

View full text Add to dashboard Cite

show abstract

“…Environmental contamination with oil (i.e. petroleum) hydrocarbons is one of the most serious ecological problems worldwide [4][5][6]. The increasing industrialization of the world's economy has led to a significant increase in consumption of petrochemical compounds [2,3].…”

Section: Introductionmentioning

confidence: 99%

Ecotoxicity and bioremediation potential assessment of soil from oil refinery station area

Zawierucha

Malina

Herman

et al. 2022

J Environ Health Sci Engineer

View full text Add to dashboard Cite

Purpose The aim of the present study was to evaluate the toxicity and biodegradation potential of oil hydrocarbons contaminated soil samples obtained from different depths at an oil refinery station area. An approach involving chemical, microbiological, respirometry and ecotoxicity assessment of soil polluted by oil hydrocarbons was adopted, in order to determine the biodegradability of pollutants and ecotoxicological effects of natural attenuation strategy. Methods The ecotoxicity of soil samples was evaluated using an ostracod test kit and a seed germination test. The results of the phytotoxicity assay were expressed as a percentage of seedling emergence and as the relative yield of fresh and dry biomass compared to control plants. The intrinsic biodegradation potential of the contaminated soil was examined using a Micro-Oxymax respirometer. Intrinsic biodegradation rates were estimated from the slopes of linear regressions curves plotted for cumulative O2 uptake. The obtained values were then entered in the mass balance equation for the stoichiometric reaction of hydrocarbon decomposition and converted per kg of soil per day. Results Although the tested contaminants were biodegradable in the respirometric assay, they were slightly to moderately toxic to plants and extremely toxic to ostracods. The noxious effects raised with the increased concentration of contaminants. The monocotyledonous oat was more tolerant to higher concentrations of oil hydrocarbons than the other test plants, indicating its greater suitability for soil reclamation purposes. Conclusion By assessing phytotoxicity and effect on ostracod mortality and progress of soil self-decontamination process, proper approach of reclamation of demoted area can be provided.

show abstract

“…Degradation of properties of soil colloids (sorption capacity, ion exchange) occurs, provoking visible and detrimental changes in the environment. Moreover, some crude oil fractions can dissolve cell membranes and consequently disrupt the structure of the plant roots [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Suitability of Melilotus officinalis for Phytoremediation of Soil Contaminated with Petroleum Hydrocarbons (TPH and PAH), Zn, Pb and Cd Based on Toxicological Tests

Steliga¹,

Kluk²

2021

Toxics

View full text Add to dashboard Cite

The article presents issues related to the possibility of using toxicological tests as a tool to monitor the progress of soil treatment contaminated with petroleum substances (TPH, PAH), Zn, Pb and Cd in bio-phytoremediation processes. In order to reduce the high content of petroleum pollutants (TPH = 56,371 mg kg−1 dry mass, PAH = 139.3 mg kg−1 dry mass), the technology of stepwise soil treatment was applied, including basic bioremediation and inoculation with biopreparations based of indigenous non-pathogenic species of bacteria, fungi and yeasts. As a result of basic bioremediation in laboratory conditions (ex-situ method), the reduction of petroleum pollutants TPH by 33.9% and PAH by 9.5% was achieved. The introduction of inoculation with biopraparation-1 prepared on the basis of non-pathogenic species of indigenous bacteria made it possible to reduce the TPH content by 86.3%, PAH by 40.3%. The use of a biopreparation-1 enriched with indigenous non-pathogenic species of fungi and yeasts in the third series of inoculation increased to an increase in the degree of biodegradation of aliphatic hydrocarbons with long carbon chains and PAH by a further 28.9%. In the next stage of soil treatment after biodegradation processes, which was characterized by an increased content of heavy metals (Zn, Pb, Cd) and naphthalene, chrysene, benzo(a)anthracene and benzo(ghi)perylene belonging to polycyclic aromatic hydrocarbons, phytoremediation with the use of Melilotus officinalis was applied. After the six-month phytoremediation process, the following was achieved: Zn content by 25.1%, Pb by 27.9%, Cd by 23.2% and TPH by 42.2% and PAH by 49.9%. The rate of removal of individual groups of hydrocarbons was in the decreasing order: C12–C18 > C6–C12 > C18–C25 > C25–C36. PAHs tended to be removed in the following order: chrysene > naphthalene > benzo(a)anthracene > benzo(ghi)perylene. The TF and BCF coefficients were calculated to assess the capacity of M. officinalis to accumulate metal in tissues, uptake from soil and transfer from roots to shoots. The values of TF translocation coefficients were, respectively, for Zn (0.44), Pb (0.12), Cd (0.40). The calculated BCF concentration factors (BCFroots > BCFshoots) show that heavy metals taken up by M. officinalis are mainly accumulated in the root tissues in the following order Zn > Pb > Cd, revealing a poor metal translocation from the root to the shoots. This process was carried out in laboratory conditions for a period of 6 months. The process of phytoremediation of contaminated soil using M. officinalis assisted with fertilization was monitored by means of toxicological tests: Microtox, Ostracodtoxkit FTM, MARA and PhytotoxkitTM. The performed phytotoxicity tests have indicated variable sensitivity of the tested plants on contaminants occurring in the studied soils, following the sequence: Lepidium sativum < Sorghum saccharatum < Sinapis alba. The sensitivity of toxicological tests was comparable and increased in the order: MARA < Ostracodtoxkit FTM < Microtox. The results of the toxicological monitoring as a function of the time of soil treatment, together with chemical analyses determining the content of toxicants in soil and biomass M. officinalis, clearly confirmed the effectiveness of the applied concept of bioremediation of soils contaminated with zinc, lead and cadmium in the presence of petroleum hydrocarbons.

show abstract

Soil Contamination with Heavy Metals and Petroleum Derivates: Impact on Edaphic Fauna and Remediation Strategies

Cited by 12 publications

References 92 publications

Laboratory-Scale Biodegradation of Fuel Oil No. 6 in Contaminated Soils by Autochthonous Bacteria

Laboratory-Scale Biodegradation of Fuel Oil No. 6 in Contaminated Soils by Autochthonous Bacteria

Ecotoxicity and bioremediation potential assessment of soil from oil refinery station area

Assessment of the Suitability of Melilotus officinalis for Phytoremediation of Soil Contaminated with Petroleum Hydrocarbons (TPH and PAH), Zn, Pb and Cd Based on Toxicological Tests

Contact Info

Product

Resources

About