Soil–Methanogen Interactions in Two Peatlands (Bog, Fen) in Central New York State

Dettling, Mark D.; Yavitt, Joseph B.; Cadillo‐Quiroz, Hinsby; Sun, Christine; Zinder, Stephen H.

doi:10.1080/01490450701456651

Cited by 27 publications

(15 citation statements)

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“…For instance, Scholten et al (2005) reported AOM in an alkaline, SO 2− 4 -rich lake mediated by a SRB, whereas known ANMEs were not involved; they suggested the SRB acts alone, meaning that yet undiscovered mechanisms might exist. For peatlands, most contain a high diversity of methanogens (Cadillo-Quiroz et al, 2008); however, ANME relatives appear to be restricted to nutrient-rich, grass dominated fen peatlands, at very low numbers, and they do not appear to occur in the extensive acidic peatlands dominated by mosses and shrubs (Dettling et al, 2007).…”

Section: Microbiologymentioning

confidence: 98%

Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

2011

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Abstract.Despite a large body of literature on microbial anaerobic oxidation of methane (AOM) in marine sediments and saline waters and its importance to the global methane (CH 4 ) cycle, until recently little work has addressed the potential occurrence and importance of AOM in non-marine systems. This is particularly true for peatlands, which represent both a massive sink for atmospheric CO 2 and a significant source of atmospheric CH 4 . Our knowledge of this process in peatlands is inherently limited by the methods used to study CH 4 dynamics in soil and sediment and the assumption that there are no anaerobic sinks for CH 4 in these systems. Studies suggest that AOM is CH 4 -limited and difficult to detect in potential CH 4 production assays against a background of CH 4 production. In situ rates also might be elusive due to background rates of aerobic CH 4 oxidation and the difficulty in separating net and gross process rates. Conclusive evidence for the electron acceptor in this process has not been presented. Nitrate and sulfate are both plausible and favorable electron acceptors, as seen in other systems, but there exist theoretical issues related to the availability of these ions in peatlands and only circumstantial evidence suggests that these pathways are important. Iron cycling is important in many wetland systems, but recent evidence does not support the notion of CH 4 oxidation via dissimilatory Fe(III) reduction or a CH 4 oxidizing archaea in consortium with an Fe(III) reducer. Calculations based on published rates demonstrate that AOM might be a significant and underappreciated constraint on the global CH 4 cycle, although much about the process is unknown, in vitro rates may not relate well to in situ rates, and projections based on those rates are fraught with uncertainty. We suggest electron transfer mechanisms, C flow and pathways, and quantifying in situ peatland AOM rates as the highest priority topics for future research.

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Section: Microbiologymentioning

confidence: 98%

Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

2011

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“…M. boonei is predicted to carry genes for three different K + uptake mechanisms including the low-affinity trk genes that many methanogens carry, in addition to the medium-affinity kup genes and the ATP-driven high-affinity kdp genes, both of which are more rarely found among methanogenic archaea (Table S1). Only one other methanogen (sequenced to date) carries all three predicted K + uptake systems, Methanosphaerula palustris E1-9c, and it was also isolated from a peatland ecosystem (Cadillo-Quiroz et al, 2009; Cadillo-Quiroz et al, 2008), a fen in which the pH was neutral but the K + porewater concentrations were only 3-8 mM (Dettling et al, 2007).…”

Section: Adaptation To High Proton Concentrationsmentioning

confidence: 99%

Genome of Methanoregula boonei 6A8 reveals adaptations to oligotrophic peatland environments

et al. 2015

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Analysis of the genome sequence of Methanoregula boonei strain 6A8, an acidophilic methanogen isolated from an ombrotrophic (rain-fed) peat bog, has revealed unique features that likely allow it to survive in acidic, nutrient-poor conditions. First, M. boonei is predicted to generate ATP using protons that are abundant in peat, rather than sodium ions that are scarce, and the sequence of a membrane-bound methyltransferase, believed to pump Na + in all methanogens, shows differences in key amino acid residues. Further, perhaps reflecting the hypokalemic status of many peat bogs, M. boonei demonstrates redundancy in the predicted potassium uptake genes trk, kdp and kup, some of which may have been horizontally transferred to methanogens from bacteria, possibly Geobacter spp. Overall, the putative functions of the potassium uptake, ATPase and methyltransferase genes may, at least in part, explain the cosmopolitan success of group E1/E2 and related methanogenic archaea in acidic peat bogs.

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“…The inhibitory concentration of total Na + for E1-9c T was nearly twice that of 'Candidatus Methanoregula boonei' (S. Bräuer, personal communication). The difference in Na + tolerance between E1-9c T and 'Candidatus Methanoregula boonei' could hypothetically reflect an adaptation to the difference in total Na + concentration observed in the pore water of Michigan Hollow (~30 mM Na + ) versus McLean Bog (~2 mM Na + ) (Dettling et al, 2007), the respective sources of each bacterium. E1-9c…”

Section: Cells Of E1-9cmentioning

confidence: 99%

Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland

Cadillo‐Quiroz

Yavitt

Zinder

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

106

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Peat-forming wetlands or peatlands (Gore, 1983) store nearly 30 % of all terrestrial carbon (Gorham, 1991) and are estimated to contribute around 20 % (115 Tg) of total annual CH 4 emissions to the atmosphere (Cicerone & Oremland, 1988). Hence, the methanogenic archaea inhabiting these ecosystems are of importance, particularly since the CH 4 concentration in the atmosphere has more than doubled in the past 200 years (Cicerone & Oremland, 1988). Surveys of 16S rRNA gene sequences from acidic and neutral pH peatlands have shown a broad diversity of euryarchaeal sequences (Cadillo-Quiroz et al., 2006Galand et al., 2003;Kotsyurbenko et al., 2004). The majority of sequences are not closely related to cultured strains, but instead form independent clusters, suggesting the existence of novel euryarchaeal groups. Two of these clusters, named groups E1 and E2 in the , 2006Galand et al., 2003; Hales et al., 1996). The order Methanomicrobiales is diverse and its members exhibit great morphological and physiological variability (Dubach & Bachofen, 1985). This order comprises the families Methanomicrobiaceae, Methanocorpusculaceae and Methanospirillaceae (Boone et al., 2001;Garcia et al., 2000). It also includes three genera that are not placed within any family: the genus Methanocalculus, which is listed as a genus incertae sedis (Boone et al., 2001), with four reported species (Lai et al., 2002Mori et al., 2000;Ollivier et al., 1998), the proposed genus 'Candidatus Methanoregula', with one species, 'Candidatus Methanoregula boonei', isolated from an acidic bog (Bräuer et al., 2006a, b), and the recently described genus Methanolinea, with one species, Methanolinea tarda, isolated from sewage sludge (Imachi et al., 2008).Abbreviations: TEM, transmission electron microscopy; T-RFLP, terminal restriction fragment length polymorphism.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and mcrA gene sequences of strain E1-9c T are EU156000 and EU296536, respectively.Plots showing the effects of sodium ion concentration on growth of strain E1-9c T and formate utilization by strain E1-9c T are available as supplementary material with the online version of this paper.

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Soil–Methanogen Interactions in Two Peatlands (Bog, Fen) in Central New York State

Cited by 27 publications

References 56 publications

Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

Genome of Methanoregula boonei 6A8 reveals adaptations to oligotrophic peatland environments

Methanosphaerula palustris gen. nov., sp. nov., a hydrogenotrophic methanogen isolated from a minerotrophic fen peatland

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