Sol–gel bioactive glasses support both osteoblast and osteoclast formation from human bone marrow cells

Karpov, Maria; Łączka, M.; Leboy, Phoebe S.; Osyczka, Anna M.

doi:10.1002/jbm.a.31386

Cited by 46 publications

(28 citation statements)

References 60 publications

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“…Our earlier studies reported the production, physico-chemical, and biological properties of gel-derived bioactive glasses of the CaO–P 2 O 5 –SiO 2 system 19,27. We showed that CaO:SiO 2 ratio in these glasses influenced their physical and chemical properties and affected the growth and differentiation of bone marrow-derived cells 6,17,20. Our data further indicate that incorporation of these gel-derived glasses into a HA matrix enhanced growth and osteogenic differentiation of human mesenchymal stem cells (MSC) 5.…”

Section: Introductionmentioning

confidence: 57%

Degradation, Bioactivity, and Osteogenic Potential of Composites Made of PLGA and Two Different Sol–Gel Bioactive Glasses

et al. 2011

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We have developed poly(l-lactide-co-glycolide) (PLGA) based composites using sol–gel derived bioactive glasses (S-BG), previously described by our group, as composite components. Two different composite types were manufactured that contained either S2—high content silica S-BG, or A2—high content lime S-BG. The composites were evaluated in the form of sheets and 3D scaffolds. Sheets containing 12, 21, and 33 vol.% of each bioactive glass were characterized for mechanical properties, wettability, hydrolytic degradation, and surface bioactivity. Sheets containing A2 S-BG rapidly formed a hydroxyapatite surface layer after incubation in simulated body fluid. The incorporation of either S-BG increased the tensile strength and Young’s modulus of the composites and tailored their degradation rates compared to starting compounds. Sheets and 3D scaffolds were evaluated for their ability to support growth of human bone marrow cells (BMC) and MG-63 cells, respectively. Cells were grown in non-differentiating, osteogenic or osteoclast-inducing conditions. Osteogenesis was induced with either recombinant human BMP-2 or dexamethasone, and osteoclast formation with M-CSF. BMC viability was lower at higher S-BG content, though specific ALP/cell was significantly higher on PLGA/A2-33 composites. Composites containing S2 S-BG enhanced calcification of extracellular matrix by BMC, whereas incorporation of A2 S-BG in the composites promoted osteoclast formation from BMC. MG-63 osteoblast-like cells seeded in porous scaffolds containing S2 maintained viability and secreted collagen and calcium throughout the scaffolds. Overall, the presented data show functional versatility of the composites studied and indicate their potential to design a wide variety of implant materials differing in physico-chemical properties and biological applications. We propose these sol–gel derived bioactive glass–PLGA composites may prove excellent potential orthopedic and dental biomaterials supporting bone formation and remodeling.

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Section: Introductionmentioning

confidence: 57%

Degradation, Bioactivity, and Osteogenic Potential of Composites Made of PLGA and Two Different Sol–Gel Bioactive Glasses

et al. 2011

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“…RANKL treatment of RAW 264.7 cells, plated on bone slices, bone-mimetics or glass slides, leads to osteoclast formation [23], [24], [25], [26], [27]. Two traditional assays used to test for osteoclast presence are the tartarate resistant acid phosphatase (TRAP) assay and bone resorption assays [7].…”

Section: Resultsmentioning

confidence: 99%

Modulation of Osteoclastogenesis with Macrophage M1- and M2-Inducing Stimuli

et al. 2014

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Macrophages are generated through the differentiation of monocytes in tissues and they have important functions in innate and adaptive immunity. In addition to their roles as phagocytes, macrophages can be further differentiated, in the presence of receptor activator of nuclear factor kappa-B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF), into osteoclasts (multinucleated giant cells that are responsible for bone resorption). In this work, we set out to characterize whether various inflammatory stimuli, known to induce macrophage polarization, can alter the type of multinucleated giant cell obtained from RANKL differentiation. Following a four-day differentiation protocol, along with lipopolysaccharide (LPS)/interferon gamma (IFNγ) as one stimulus, and interleukin-4 (IL-4) as the other, three types of multinucleated cells were generated. Using various microscopy techniques (bright field, epifluorescence and scanning electron), functional assays, and western blotting for osteoclast markers, we found that, as expected, RANKL treatment alone resulted in osteoclasts, whereas the addition of LPS/IFNγ to RANKL pre-treated macrophages generated Langhans-type giant cells, while IL-4 led to giant cells resembling foreign body giant cells with osteoclast-like characteristics. Finally, to gain insight into the modulation of osteoclastogenesis, we characterized the formation and morphology of RANKL and LPS/IFNγ-induced multinucleated giant cells.

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“…A separate study on 45S5 Bioglass 45S5 discs showed no significant difference on differentiation of human-bone-marrow-derived MSCs compared to those cultured on tissue culture plastic (with or without bone morphogenetic protein 2, BMP-2) [129]. When bone marrow MSCs were cultured on bioactive sol-gel coatings with low silica content (40 mol.% SiO 2 , 54 mol.% CaO, 6 mol.% P 2 O 5 ), the MSCs differentiated into osteoblasts and osteoclasts (with or without BMP-2 added to the culture) [130], which seems an ideal result for bone regeneration (bone production and remodelling). When the cells were cultured on glass coatings of high silica composition (80 mol.% SiO 2 , 54 mol.% CaO, 6 mol.% P 2 O 5 ), the MSCs differentiated into osteoblasts but not osteoclasts.…”

Section: Ionic Dissolution Products and Osteogenesismentioning

confidence: 99%