Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

Solanki, Pratima R.; Patel, M.; Pandey, Manoj Kumar; Kotnala, R.K.; Malhotra, Bansi D.

doi:10.1002/elan.201100351

Cited by 22 publications

(12 citation statements)

References 21 publications

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“…An analytical device for cholera detection based on antibody-conjugated ZrO 2 NPs has recently been reported 29 . In another study, an electrochemcial gold electrode modified with polytryamine was found to be sensitive at attomolar concentrations of cholera 30 .…”

Section: Discussionmentioning

confidence: 99%

A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection

Patel

Ali

Krishnan

et al. 2015

Sci Rep

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Nanomaterial-based photoluminescence (PL) diagnostic devices offer fast and highly sensitive detection of pesticides, DNA, and toxic agents. Here we report a label-free PL genosensor for sensitive detection of Vibrio cholerae that is based on a DNA hybridization strategy utilizing nanostructured magnesium oxide (nMgO; size >30 nm) particles. The morphology and size of the synthesized nMgO were determined by transmission electron microscopic (TEM) studies. The probe DNA (pDNA) was conjugated with nMgO and characterized by X-ray photoelectron and Fourier transform infrared spectroscopic techniques. The target complementary genomic DNA (cDNA) isolated from clinical samples of V. cholerae was subjected to DNA hybridization studies using the pDNA-nMgO complex and detection of the cDNA was accomplished by measuring changes in PL intensity. The PL peak intensity measured at 700 nm (red emission) increases with the increase in cDNA concentration. A linear range of response in the developed PL genosensor was observed from 100 to 500 ng/μL with a sensitivity of 1.306 emi/ng, detection limit of 3.133 ng/μL and a regression coefficient (R2) of 0.987. These results show that this ultrasensitive PL genosensor has the potential for applications in the clinical diagnosis of cholera.

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Section: Discussionmentioning

confidence: 99%

A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection

Patel

Ali

Krishnan

et al. 2015

Sci Rep

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“…5,6 Carbon allotropes, like graphene oxide (GO), fullerenes, carbon nanotubes, etc., have been explored for cellular imaging, tissue engineering, intercalation materials, drug delivery biomaterials and biosensors. [7][8][9][10][11] For biosensor fabrication, the presence of available functional groups (e.g. hydroxyl, epoxide, carbonyl, carboxyl, etc.)…”

Section: Introductionmentioning

confidence: 99%

Reduced graphene oxide–titania based platform for label-free biosensor

et al. 2014

Self Cite

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A label-free biosensor has been fabricated using a reduced graphene oxide (RGO) and anatase titania (antTiO 2 ) nanocomposite, electrophoretically deposited onto an indium tin oxide coated glass substrate. The RGO-ant-TiO 2 nanocomposite has been functionalized with protein (horseradish peroxidase) conjugated antibodies for the specific recognition and detection of Vibrio cholerae. The presence of Ab-Vc on the RGO-ant-TiO 2 nanocomposite has been confirmed using electron microscopy, Fourier transform infrared spectroscopy and electrochemical techniques. Electrochemical studies relating to the fabricated Ab-Vc/RGO-ant-TiO 2 /ITO immunoelectrode have been conducted to investigate the binding kinetics.This immunosensor exhibits improved biosensing properties in the detection of Vibrio cholerae, with a sensitivity of 18.17 Â 10 6 F mol À1 L À1 m À2 in the detection range of 0.12-5.4 nmol L À1 , and a low detection limit of 0.12 nmol L À1 . The association (k a ), dissociation (k d ) and equilibrium rate constants have been estimated to be 0.07 nM, 0.002 nM and 0.41 nM, respectively. This Ab-Vc/RGO-ant-TiO 2 /ITO immunoelectrode could be a suitable platform for the development of compact diagnostic devices.

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“…This represents a healthy research activity with a 16% growth rate annually [ 79 ]. Biosensors should offer quick and efficient detection of viral diseases with high levels of specificity and sensitivity [ 80 ]. These criteria are crucial in the success or failure of the detection technology.…”

Section: How Are Biosensors and Bioelectronics Beneficial For Virus Dmentioning

confidence: 99%

How should diagnostic kits development adapt quickly in COVID 19-like pandemic models? Pros and cons of sensory platforms used in COVID-19 sensing

Moulahoum

Ghorbanizamani

Zihnioğlu

et al. 2021

Talanta

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As COVID-19 has reached pandemic status and the number of cases continues to grow, widespread availability of diagnostic testing is critical in helping identify and control the emergence of this rapidly spreading and serious illness. However, a lacking in making a quick reaction to the threat and starting early development of diagnostic sensing tools has had an important impact globally. In this regard, here we will review critically the current developed diagnostic tools in response to the COVID-19 pandemic and compare the different types through the discussion of their pros and cons such as nucleic acid detection tests (including PCR and CRISPR), antibody and protein-based diagnosis tests. In addition, potential technologies that are under development such as on-site diagnosis platforms, lateral flow, and portable PCR units are discussed. Data collection and epidemiological analysis could also be an interesting factor to incorporate with the emerging technologies especially with the wide access to smartphones. Lastly, a SWOT analysis and perspectives on how the development of novel sensory platforms should be treated by the different decision-makers are analyzed.

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Sol‐Gel Derived Nanostructured Metal Oxide Platform for Bacterial Detection

Cited by 22 publications

References 21 publications

A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection

A Label-Free Photoluminescence Genosensor Using Nanostructured Magnesium Oxide for Cholera Detection

Reduced graphene oxide–titania based platform for label-free biosensor

How should diagnostic kits development adapt quickly in COVID 19-like pandemic models? Pros and cons of sensory platforms used in COVID-19 sensing

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