We review the wide range of astrophysical observations of gamma ray emission lines and we discuss their implications. We consider line emission from solar flares, the Orion molecular cloud complex, supernovae 1987A and 1991T, the supernova remnants Cas A and Vela, the interstellar medium, the Galactic center region and several Galactic black hole candidates. The observations have important, and often unique, implications on particle acceleration, star formation, processes of nucleosynthesis, Galactic evolution and compact object physics.