Solid-lipid nanoparticle formulation improves antiseizure action of cryptolepine

Mante, Priscilla Kolibea; Adomako, Nana Ofori; Antwi, Paulina; Kusi-Boadum, Nana Kofi; Osafo, Newman

doi:10.1016/j.biopha.2021.111354

Cited by 17 publications

(8 citation statements)

References 53 publications

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“…However, Dexamethasone remained superior to SLN-CRYP in its anti-inflammatory effect. Compared to cryptolepine, the higher anti-inflammatory effect of SLN-CRYP in brain tissues may be due to increased BBB penetration of the nanoparticle formulation of cryptolepine as was previously shown by the authors ( Mante et al., 2021 ).…”

Section: Discussionsupporting

confidence: 54%

“…Extraction of cryptolepine (Cryp) from Cryptolepis sanguinolenta was done as described by Mante et al. (2021) .…”

Section: Methodsmentioning

confidence: 99%

“…Cryptolepine (Cryp) is known to possess anti-inflammatory properties ( Bugyei et al., 2010 ). The authors have previously shown that the SLN formulation of cryptolepine aids the molecule to cross the blood brain barrier and exert improved anti-seizure activity ( Mante et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Chronic administration of cryptolepine nanoparticle formulation alleviates seizures in a neurocysticercosis model

Mante

Adomako

Antwi

et al. 2021

Current Research in Pharmacology and Drug Discovery

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Worldwide, neurocysticercosis remains an important cause of acquired epilepsy. We therefore seek to investigate the effectiveness of the nanoparticle formulation of cryptolepine in alleviating seizures in a neurocysticercosis model. A solid-lipid nanoparticle formulation of extracted cryptolepine was prepared. The parasites were maintained in T. crassiceps metacestode (ORF strain) - infected female BALB/c mice. Cryp (5 mg/kg), SLN-CRYP (5 mg/kg), ABZ (50 mg/kg) DXM (0.5 mg/kg), and PHE (30 mg/kg). were assessed for in vitro cysticidal, in vivo cysticidal and/or antiseizure activity in 70 mice that had developed seizures from infection with T. crassiceps. General pathologic processes were studied in the host tissue and inflammatory mediators were quantified from isolated mice brains. All treatments (CRYP, SLN-CRYP and ABZ) caused significantly reduced viability of T. crassiceps cysts. Treatment with SLN-CRYP significantly shrunk cysticerci and resolved ventricular expansion and deviation similar to albendazole on examination of encephala. SLN-CRYP inhibited hyperemia but was more effective against microgliosis, calcification, edema and meningitis. Mean seizure score was significantly reduced in models administered with SLN-CRYP (p < 0.0001); as were frequency (p < 0.0001) and duration (p < 0.0001) of seizures. SLN-CRYP significantly reduced brain homogenate levels of IL-10 (p = 0.0016) and IFN-γ (p < 0.0001). Our study shows that the chronic administration of the nanoparticle formulation of cryptolepine is effective in alleviating seizures associated with neurocysticercosis in a mouse model.

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Section: Discussionsupporting

confidence: 54%

“…Extraction of cryptolepine (Cryp) from Cryptolepis sanguinolenta was done as described by Mante et al. (2021) .…”

Section: Methodsmentioning

confidence: 99%

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Chronic administration of cryptolepine nanoparticle formulation alleviates seizures in a neurocysticercosis model

Mante

Adomako

Antwi

et al. 2021

Current Research in Pharmacology and Drug Discovery

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“…Kashyap et al, reported nanoformulation-based delivery of various herbal bioactive compounds and evaluated them for in vivo anticancer activity as well as in a cell line study [ 9 ]. There are various approaches reported for increasing the solubility and therapeutic activity of poorly soluble drugs like cryptolepine-loaded solid lipid nanoparticles [ 10 ], black soybean nanoparticles [ 11 ], epicatechin and hydrogenated soybean-loaded hybrid nanoparticles [ 12 ], clofazimine and gefitinib-loaded polymeric nanoparticles [ 13 , 14 ]. The nanoparticle is one of the best ways to increase the solubility and therapeutic efficacy of low-soluble bioactive therapeutics.…”

Section: Introductionmentioning

confidence: 99%

Development and Optimization of Hybrid Polymeric Nanoparticles of Apigenin: Physicochemical Characterization, Antioxidant Activity and Cytotoxicity Evaluation

Zafar

Alruwaili

Imam

et al. 2022

Sensors

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Breast cancer is the most common cancer in females and ranked second after skin cancer. The use of natural compounds is a good alternative for the treatment of breast cancer with less toxicity than synthetic drugs. The aim of the present study is to develop and characterize hybrid Apigenin (AN) Nanoparticles (NPs) for oral delivery (AN-NPs). The hybrid AN-NPs were prepared by the self-assembly method using lecithin, chitosan and TPGS. Further, the NPs were optimized by Box-Behnken design (3-factor, 3-level). The hybrid NPs were evaluated for particle size (PS), entrapment efficiency (EE), zeta potential (ZP), and drug release. The optimized hybrid NPs (ON2), were further evaluated for solid state characterization, permeation, antioxidant, cytotoxicity and antimicrobial study. The formulation (ON2) exhibited small PS of 192.6 ± 4.2 nm, high EE 69.35 ± 1.1%, zeta potential of +36.54 mV, and sustained drug release (61.5 ± 2.5% in 24 h), as well as significantly (p < 0.05) enhanced drug permeation and antioxidant activity. The IC50 of pure AN was found to be significantly (p < 0.05) lower than the formulation (ON2). It also showed significantly greater (p < 0.05) antibacterial activity than pure AN against Bacillus subtilis and Salmonella typhimurium. From these findings, it revealed that a hybrid AN polymeric nanoparticle is a good carrier for the treatment of breast cancer.

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“…Furthermore, nano scaled carriers grant selective and targeted drug delivery to the brain reducing hazardous effects on healthy tissue [15]. Previous studies clarified the therapeutic antiepileptic effect of nanocarriers as liposomes and polymeric or lipid nanoparticles in animal models of epilepsy [16,17]. Finetuning of nanosystem structure, composition and physicochemical properties affect the cellular uptake and response to different nanoparticles [18].…”

Section: Introductionmentioning

confidence: 99%

Brain targeted lactoferrin coated lipid nanocapsules for the combined effects of apocynin and lavender essential oil in PTZ induced seizures

Youssef,

Boraie,

Ismail

et al. 2024

Drug Deliv. and Transl. Res.

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Apocynin (APO) is a plant derived antioxidant exerting specific NADPH oxidase inhibitory action substantiating its neuroprotective effects in various CNS disorders, including epilepsy. Due to rapid elimination and poor bioavailability, treatment with APO is challenging. Correspondingly, novel APO-loaded lipid nanocapsules (APO-LNC) were formulated and coated with lactoferrin (LF-APO-LNC) to improve br ain targetability and prolong residence time. Lavender oil (LAV) was incorporated into LNC as a bioactive ingredient to act synergistically with APO in alleviating pentylenetetrazol (PTZ)-induced seizures. The optimized LF-APO-LAV/LNC showed a particle size 59.7 ± 4.5 nm with narrow distribution and 6.07 ± 1.6mV zeta potential) with high entrapment efficiency 92 ± 2.4% and sustained release (35% in 72 h). Following subcutaneous administration, LF-APO-LAV/LNC brought about ⁓twofold increase in plasma AUC and MRT compared to APO. A Log BB value of 0.2 ± 0.14 at 90 min reflects increased brain accumulation. In a PTZ-induced seizures rat model, LF-APO-LAV/LNC showed a Modified Racine score of 0.67 ± 0.47 with a significant increase in seizures latency and decrease in duration. Moreover, oxidant/antioxidant capacity and inflammatory markers levels in brain tissue were significantly improved. Histopathological and immunohistochemical assessment of brain tissue sections further supported these findings. The results suggest APO/LAV combination in LF-coated LNC as a promising approach to counteract seizures. Graphical Abstract

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Solid-lipid nanoparticle formulation improves antiseizure action of cryptolepine

Cited by 17 publications

References 53 publications

Chronic administration of cryptolepine nanoparticle formulation alleviates seizures in a neurocysticercosis model

Chronic administration of cryptolepine nanoparticle formulation alleviates seizures in a neurocysticercosis model

Development and Optimization of Hybrid Polymeric Nanoparticles of Apigenin: Physicochemical Characterization, Antioxidant Activity and Cytotoxicity Evaluation

Brain targeted lactoferrin coated lipid nanocapsules for the combined effects of apocynin and lavender essential oil in PTZ induced seizures

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