Solid-Phase Enzyme Immunoassay for Herpes Simplex Virus

Abstract

An enzyme immunoassay was used for the rapid detection of herpes simplex virus type 1 (HSV-1). The test utilized specific antibodies to HSV-1 attached to a solid phase. A laboratory preparation of HSV-1 was detectable in amounts of greater than or equal to 50 50% tissue culture-infective doses by this method. Tests performed with clinical samples indicated a specificity of 95%. The sensitivity appeared to depend on the length of time the samples were stored. When samples were stored frozen for three to six months, the sensitivity was 46% of that of tissue culture isolations performed when the samples were first collected. The sensitivity was 89% in comparison with attempts at tissue culture reisolation with the same samples at the time of enzyme immunoassay. The immunoassay also detected HSV in seven of 10 positive samples that were stored frozen for no more than two weeks before testing.

Enzyme immunoassays with special reference to ELISA techniques.

“…There are few other reports of ELISA being used to detect viruses in clinical specimens, but Miranda et al (1977) indicated that there is considerable promise in detecting herpes simplex virus in such material. The demonstration of antigen may well become more important in other infectious diseases now that sensitive methods are available.…”

Rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin-streptavidin enzyme-linked immunosorbent assay

“…(Parts of this work were presented at the Second Annual Meeting of the Society for Virology, East Lansing, Mich., [10][11][12][13][14] July 1983, and at the Workshop on New and Useful Methods in Rapid Viral Diagnosis, organized by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, and Pan American Group for Rapid Viral Diagnosis in Bethesda, Md., 29-30 September 1983. ) MATERIALS AND METHODS Preparation of stock virus.…”

Detection and Quantification of Foot and Mouth Disease Virus by Enzyme Labelled Immunosorbent Assay Techniques

“…The micro ELISA test is frequently used for the detection and quantification of viruses; (Voller et al 1976;Wolters et al I976, I977;Halbert & Anken, 1977;Miranda et al 1977;Yolken et al ~977). The technique had been found to be rapid and can be as sensitive as radioimmunoassay (RIA), and makes it possible to detect small amounts of material without the problems associated with the use of radioactive compounds.…”