Solid-phase extraction of vinblastine and vincristine from plasma and urine: variable drug recoveries due to non-reproducible column packings

Vendrig, D. E. M. M.; Holthuis, J.J.M.; Erdélyi-Tóth, V.; Hulshoff, A.

doi:10.1016/0378-4347(87)80027-0

Cited by 39 publications

(7 citation statements)

References 7 publications

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“…Already by these few reports the versatility of SPE was demonstrated. Vendrig et al [201] however, reported, variable recoveries of dimeric indole alkaloids (vinblastine and vincristine) due to non-reproducible diol-column packings.…”

Section: Analysis Of Alkaloid Productionmentioning

confidence: 99%

Cell and tissue cultures ofCatharanthus roseus (L.) G. Don: a literature survey

Heijden

Verpoorte

Hoopen

1989

Plant Cell Tiss Organ Cult

131

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The literature concerning the formation of secondary metabolites in cell and tissue cultures of Catharanthus roseus has been reviewed. Several aspects involved in the formation of secondary metabolites are discussed; e.g. regulation of secondary metabolism, environmental factors influencing secondary metabolism, biosynthesis and enzymology of the products, analysis of product formation, immobilization of cultured cells and stability of cell lines. Some economical aspects of production processes are discussed.

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Section: Analysis Of Alkaloid Productionmentioning

confidence: 99%

Cell and tissue cultures ofCatharanthus roseus (L.) G. Don: a literature survey

Heijden

Verpoorte

Hoopen

1989

Plant Cell Tiss Organ Cult

131

View full text Add to dashboard Cite

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“…Since vincristine lacks both strong UV absorption and electrochemical response, large volumes [0.5-4 ml] of plasma or serum are needed to achieve the desired sensitivity for pharmacokinetic studies with these methods [10,[12][13][14][15]. By using UV detection at 297 nm and solid-phase extraction of 500 l of plasma, a lower limit of quantitation of 28.6 ng/ml in plasma was achieved [10].…”

Section: Introductionmentioning

confidence: 98%

“…By using UV detection at 297 nm and solid-phase extraction of 500 l of plasma, a lower limit of quantitation of 28.6 ng/ml in plasma was achieved [10]. HPLC-electrochemical detection assays using solid-phase extraction and large sample volumes [1-1.2 ml] led to the detection of 100-300 pg of vincristine on-column at a signal-to-noise ratio of 3 [11][12][13][14]. One electrochemical based HPLC method reported a lower limit of quantitation of 0.48 ng/ml by using an online solid-phase extraction and 300 l of plasma [11].…”

Section: Introductionmentioning

confidence: 99%

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Determination of vincristine in mouse plasma and brain tissues by liquid chromatography–electrospray mass spectrometry

Guo

Wang

Zhou

et al. 2004

Journal of Chromatography B

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“…12 For bioanalytical quantification of vincristine methods have been described using a radioimmunoassay, 6 HPLC coupled to UV detection, 13,14 electrochemical detection (ECD), [15][16][17][18][19] electrospray ionization (ESI) mass spectrometry 20,21 or atmospheric pressure chemical ionization (APCI) mass spectrometry. [22][23][24] Due to high sensitivity and selectivity liquid chromatography coupled on-line to triple quadrupole tandem mass spectrometry (LC/MS/MS) has become the first choice for the analysis of anticancer drugs in biological matrices.…”

mentioning

confidence: 99%

Validated assay for the simultaneous quantification of total vincristine and actinomycin‐D concentrations in human EDTA plasma and of vincristine concentrations in human plasma ultrafiltrate by high‐performance liquid chromatography coupled with tandem mass spectrometry

Damen

Israëls

Caron

et al. 2009

Rapid Comm Mass Spectrometry

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A sensitive, specific and efficient high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) assay for the simultaneous determination of total vincristine and actinomycin-D concentrations in human plasma and an assay for the determination of unbound vincristine are presented. Electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and heated electrospray ionization (H-ESI) were tested as ionization interfaces. For reasons of robustness ESI was chosen followed by tandem mass spectrometry (ESI-MS/MS). For the plasma assay a 30 microL aliquot was protein precipitated with acetonitrile/methanol (50:50, v/v) containing the internal standard vinorelbine and 10 microL volumes were injected onto the HPLC system. To determine unbound vincristine, ultrafiltrate was produced from plasma using 30 kDa centrifugal filter units. The plasma ultrafiltrate was mixed with methanol (50:50, v/v), internal standard vinorelbine was added and 20 microL aliquots were injected onto the HPLC system. Separation was achieved on a 50x2.1 mm i.d. Xbridge C18 column using 1 mM ammonium acetate/acetonitrile (30:70, v/v) adjusted to pH 10.5 with ammonia, run in a gradient with methanol at a flow rate of 0.4 mL/min. HPLC run time was 6 min. The assay quantifies in plasma vincristine from 0.25 to 100 ng/mL and actinomycin-D from 0.5 to 250 ng/mL using plasma sample volumes of only 30 microL. Vincristine in plasma ultrafiltrate can be quantified from 1 to 100 ng/mL. Validation results demonstrate that vincristine and actinomycin-D can be accurately and precisely quantified in human plasma and plasma ultrafiltrate with the presented methods. The assays are now in use to support clinical pharmacological studies in children treated with vincristine and actinomycin-D.

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Solid-phase extraction of vinblastine and vincristine from plasma and urine: variable drug recoveries due to non-reproducible column packings

Cited by 39 publications

References 7 publications

Cell and tissue cultures ofCatharanthus roseus (L.) G. Don: a literature survey

Cell and tissue cultures ofCatharanthus roseus (L.) G. Don: a literature survey

Determination of vincristine in mouse plasma and brain tissues by liquid chromatography–electrospray mass spectrometry

Validated assay for the simultaneous quantification of total vincristine and actinomycin‐D concentrations in human EDTA plasma and of vincristine concentrations in human plasma ultrafiltrate by high‐performance liquid chromatography coupled with tandem mass spectrometry

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