Solid-Phase Synthesis of a Special Phosphorylated Peptide as a Biomarker for LC–MS/MS Detection for OPNA Exposure

Abstract

A synthesis of d 5 -VX adducted nonapeptide via solid-phase approach has been developed. The d 5 -VX peptide could be used as the isotope-labeled internal standard for LC-MS/MS detecting the BuChE-OPNA biomarkers. The Kaiser test was utilized to ensure the right connections of all of the amino acids. This method offers an access to the synthesis and detection of other phosphorylated nonapeptides.