Solid State Cultivation of Hericium erinaceus Biomass and Erinacine: A Production

Abstract

Hericium erinaceus is a medicinal mushroom producing biologically active metabolite erinacin A. In this research we compared fungal growth and metabolite production in different reactor types and different substrates with added casein peptone and NaCl. Cultivation of fungal biomass was performed in glass jars and horizontal stirred tank reactor. The cultivation in reactor was carried out at 24°C and airflow 5 L/min. Periodical mixing of N = 80 rpm, was used. Solid state substrate based on mixture of husked millet and paddy millet was used. For simultaneous determination of erinacine A and ergosterol contents in a single sample new analytical method was developed. The amount of biomass obtained in glass jars averaged at 100 mg/g ± 12 mg/g, while in horizontal mixing bioreactor it easily raised up to 350 mg/g. The highest erinacin A concentration was detected in substrate with 0.56% NaCl and 3.4% casein peptone content.

Hericium erinaceus Improves Recognition Memory and Induces Hippocampal and Cerebellar Neurogenesis in Frail Mice during Aging

“…Lyophilized mycelium and sporophores of He1 were extracted in 70% ethanol, per the procedure described by Gerbec et al [39]. In details, one gram of dry substrate was blended with 10 mL of 70% ethanol and left in the thermostat overnight at 50 °C.…”

Array of Metabolites in Italian Hericium erinaceus Mycelium, Primordium, and Sporophore

“…The procedure of alcoholic extraction described by Lee et al (2016) and Gerbec et al (2015) [18,42] was followed with slight modification: 1 g of lyophilized mycelium/dried WT/cultivated sporophores/fresh primordium was blended with 10 mL of ethanol 70% and left in the thermostat at 50 °C for 24 h. At the end, the material was transferred for centrifugation (4000 rpm for 3 min) and the supernatant was stored at −20 °C for HPLC analysis.…”

Neuroprotective Metabolites of Hericium erinaceus Promote Neuro-Healthy Aging

“…In detail, 1 g of lyophilized mycelium or sporophore of He1 was blended with 10 mL of ethanol 70%, and left in the thermostat overnight at 50 C. Before withdrawing, the material was stirred for one hour and was centrifuged at 4000 rpm for three minutes. The supernatant was stored at 20 • C. The detailed procedures were previously described by Ratto et al, Lee et al, and Gerbec et al [14,52,99,100].…”