Solid-state NMR spectroscopy reveals anomer specific transport of galactose in the milk yeast Kluyveromyces lactis

Fukasawa, Toshio; Abe, Akio; Nakamura, Atsusi; Horigome, Miyako; Naito, Akira

doi:10.1111/j.1567-1364.2012.00788.x

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…In contrast to bacterial (Fukasawa et al, 2012 ) and mammalian (Park et al, 2007 ) AEPs, the structure and properties of mutarotases of higher plants are not known. NbAELP is probably involved in the metabolism of sugars as suggested by the decreased glucose levels in the leaves of transgenic plants with a decreased level of NbAELP mRNA (Figure 5A ).…”

Section: Discussionmentioning

confidence: 99%

The Intergenic Interplay between Aldose 1-Epimerase-Like Protein and Pectin Methylesterase in Abiotic and Biotic Stress Control

et al. 2017

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The mechanical damage that often precedes the penetration of a leaf by a pathogen promotes the activation of pectin methylesterase (PME); the activation of PME leads to the emission of methanol, resulting in a “priming” effect on intact leaves, which is accompanied by an increased sensitivity to Tobacco mosaic virus (TMV) and resistance to bacteria. In this study, we revealed that mRNA levels of the methanol-inducible gene encoding Nicotiana benthamiana aldose 1-epimerase-like protein (NbAELP) in the leaves of intact plants are very low compared with roots. However, stress and pathogen attack increased the accumulation of the NbAELP mRNA in the leaves. Using transiently transformed plants, we obtained data to support the mechanism underlying AELP/PME-related negative feedback The insertion of the NbAELP promoter sequence (proNbAELP) into the N. benthamiana genome resulted in the co-suppression of the natural NbAELP gene expression, accompanied by a reduction in the NbAELP mRNA content and increased PME synthesis. Knockdown of NbAELP resulted in high activity of PME in the cell wall and a decrease in the leaf glucose level, creating unfavorable conditions for Agrobacterium tumefaciens reproduction in injected leaves. Our results showed that NbAELP is capable of binding the TMV movement protein (MPTMV) in vitro and is likely to affect the cellular nucleocytoplasmic transport, which may explain the sensitivity of NbAELP knockdown plants to TMV. Although NbAELP was primarily detected in the cell wall, the influence of this protein on cellular PME mRNA levels might be associated with reduced transcriptional activity of the PME gene in the nucleus. To confirm this hypothesis, we isolated the N. tabacum PME gene promoter (proNtPME) and showed the inhibition of proNtPME-directed GFP and GUS expression in leaves when co-agroinjected with the NbAELP-encoding plasmid. We hypothesized that plant wounding and/or pathogen attack lead to PME activation and increased methanol emission, followed by increased NbAELP expression, which results in reversion of PME mRNA level and methanol emission to levels found in the intact plant.

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Section: Discussionmentioning

confidence: 99%

The Intergenic Interplay between Aldose 1-Epimerase-Like Protein and Pectin Methylesterase in Abiotic and Biotic Stress Control

et al. 2017

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“…The resulting high-resolution solid-state SPE spectra, unprecedented for apple CW, allowed a detailed insight into the physical and chemical properties of very mobile polysaccharides such as the arabinan and galactan side chains of the pectic polysaccharide rhamnogalacturonan I. ssNMR spectroscopy has been successfully applied also to monitor hydration properties of cellulose ( 2 H and 13 C ssNMR) [27], structure determination of waterinsoluble products [28], and characterization of new solid form of trehalose [29] or detect effects of cooking on the CW [30]. Fukasawa et al [31] studied transportation mechanism of galactose in the milk yeast Kluyveromyces lactis.…”

Section: Solid-state Nmrmentioning

confidence: 98%

Recent Advances in NMR Studies of Carbohydrates

Buda¹,

Nawój²,

Młynarski³

2016

Annual Reports on NMR Spectroscopy

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l-Arabinose induces d-galactose catabolism via the Leloir pathway in Aspergillus nidulans

Németh

Kulcsár

Flipphi

et al. 2019

Fungal Genetics and Biology

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L-Arabinose and D-galactose are the principal constituents of L-arabinogalactan, and also cooccur in other hemicelluloses and pectins. In this work we hypothesized that similar to the induction of relevant glycoside hydrolases by monomers liberated from these plant heteropolymers, their respective catabolisms in saprophytic and phytopathogenic fungi may respond to the presence of the other sugar to promote synergistic use of the complex growth substrate. We showed that these two sugars are indeed consumed simultaneously by Aspergillus nidulans, while L-arabinose is utilised faster in the presence than in the absence of D-galactose. Furthermore, the first two genes of the Leloir pathway for D-galactose catabolismencoding D-galactose 1-epimerase and galactokinaseare induced more rapidly by L-arabinose than by D-galactose eventhough deletion mutants thereof grow as well as a wild type strain on the pentose. D-Galactose 1-epimerase is hyperinduced by L-arabinose, Dxylose and L-arabitol but not by xylitol. The results suggest that in A. nidulans, L-arabinose and D-xyloseboth requiring NADPH for their catabolisationactively promote the enzyme infrastructure necessary to convert -D-galactopyranose via the Leloir pathway with its anomer specific enzymes, into -D-glucose-6-phosphate (the starting substrate of the oxidative part of the pentose phosphate pathway) even in the absence of D-galactose.

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Solid-state NMR spectroscopy reveals anomer specific transport of galactose in the milk yeast Kluyveromyces lactis

Cited by 4 publications

References 26 publications

The Intergenic Interplay between Aldose 1-Epimerase-Like Protein and Pectin Methylesterase in Abiotic and Biotic Stress Control

The Intergenic Interplay between Aldose 1-Epimerase-Like Protein and Pectin Methylesterase in Abiotic and Biotic Stress Control

Recent Advances in NMR Studies of Carbohydrates

l-Arabinose induces d-galactose catabolism via the Leloir pathway in Aspergillus nidulans

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