Solid State NMR Study ofCouma utilisSeeds

Abstract

This article studies the seed of sorva (Couma utilis) that is a native fruit of Amazons State, in Brazil. Sorva seed components were analyzed by 1 H and 13 C using solid state high resolution nuclear magnetic resonance (NMR) spectroscopy, because they are important nuclei to be used to evaluate plant seeds. High resolution 1 H NMR ( 1 H HRMAS) with magic angle sample spinning (MAS), and 13 C NMR using MAS and particularly 13 C NMR with cross-polarization (CP) and MAS are techniques that allow the evaluation of the seed in detail. The variable contact-time (VCT) experiment is another useful technique that can be used to measure a proton spin-lattice relaxation time in the rotating frame, which is a parameter that gives response on the intermolecular interaction and is related to the sample spatial homogeneity. From the NMR results it was found that the sorva seeds are mainly constituted of polysaccharides, glycoproteins, and triacylglycerides.

The NMR Study of the Latex Extracted from Brosimum Parinarioides

“…From the analyses of these NMR signals the majority of components of the latex is derived from: cisisoprene, saturated and unsaturated fatty acids and triacylglycerides esters. As minor components terpenoid compounds were detected and the structure of acyl lupeol ester [2] was identified. The same type of components has been characterized in a similar sample, studied before [2][3][4]15].…”

“…Natural latexes are complex emulsion in which many substances, such as proteins, polysaccharides, sugars, oils, gums and cis-1,4polyisoprene can be found as major constituents. The latex extracted from Brosimum parinarioides tree found in Amazônia crops is frequently used as food for humble people and it is also used as a popular medicine for treating tuberculosis and asthma [1][2][3][4]. Since its uses were known by the scientific community, some studies about this latex started, but no study was done deeply to know the major components present in these types of sample.…”

“…It is known that nuclear magnetic resonance is very much used to characterize a chemical structure and a molecular dynamic of samples [2][3][4][5][6][7][8][9][10][11][12][13][14][15]. This spectroscopy has many techniques, which differ in pulse sequence that permits us to evaluate both chemical constituents and molecular dynamic, because NMR analyzes the sample in different physical states [2][3][4][10][11][12][13][14][15]. According to the context, this work has a purpose to map the principal components that are responsible for the pharmacological and toxic effects and determine the stability degree of this latex after being kept at environment conditions.…”

The Use of Low‐Field Solid‐State NMR Relaxation to Study the Latex Extracted from Brosimum Parinarioides

“…[1][2][3][4] However, fundamental studies about the chemical characteristics of Leite de Amapá have not been reported so far.…”

“…[2][3][4][5][6][7][8][9][10][11][12][13][14][15] Particularly, NMR analyses have been employed successfully to characterize the chemical composition of latexes, using different pulse sequences in high and low magnetic fields. [2][3][4][10][11][12][13][14][15] Based on the previous remarks, the main objective of the present work was to monitor the stability of the Brosimum parinarioides latex at storage conditions at ambient temperature for a period of twelve months. In order to do that, low-field NMR analyses were used to determine the hydrogen relaxation parameters and monitor the stability of the latex.…”