Solubilized candida cell Wall β‐glucan, CSBG, is an epitope of natural human antibody

Abstract

We have recently developed a protocol to obtain a soluble Candida spp. b1, 3-glucan (CSBG) by sodium hypochlorite (NaClO) oxidation and subsequent dimethyl sulfoxide (DMSO) extraction. CSBG is composed mainly of b-1,3 and b-1,6-glucosidic linkages with a small amount of branching. Antibody to b-glucan is generally difficult to produce. In the present study, the specificity of the human sera to CSBG was examined by ELISA. Using CSBG-coated plates, sera from normal human volunteers showed high reactivity that was neutralized by adding soluble CSBG as a competitor. However, the reactivity could not be neutralized by a b-1,6 branched b-1,3-glucan, grifolan. Similar specificity of the immunoglobulin (Ig) was detected in commercially available g-globulin prepared from pooled human sera. Comparing various standard glucans, a major epitope was present on b-1,6 glucan segment. Drug Dev.

Antibody to Soluble 1,3/1,6-.BETA.-D-Glucan, SCG in Sera of Naive DBA/2 Mice

“…On the other hand, we recently reported that the antibody to solubilized Candida cell wall b-glucan (CSBG) having b-1,3 and b-1,6glucosidic linkages, was detected in sera of Candida immune mice and human sera. 8,9) From this evidence, it was suggested that not all b-glucans were weak immunogens.…”

Imprime PGG-Mediated Anti-Cancer Immune Activation Requires Immune Complex Formation

“…Since ABA levels vary across the human population [11–13], we sought to characterize whether differences in ABA levels may influence Imprime binding. In sera from 143 healthy human volunteer subjects, binding of Imprime in WB was correlated to IgG (independent of IgM) or IgM (independent of IgG) ABA levels measured in the serum from the same blood draw.…”

Strategy of Infection Control in Immunosuppressive Therapy for ANCA-Associated Vasculitis

“…7) The anti-CSBG antibody recognizes the straight chain b6 structure of b glucan, and its specific immune responses to b glucan were confirmed using an inhibition test. As shown in Fig.…”

“…The measurement of the anti-CSBG antibody in patients with AAV is useful for evaluating the natural or acquired immune capacity of the host against b glucan and predicting the development of deep-seated mycosis as a complication, and this antibody titer can be a clinical parameter for optimal immunosuppressive therapy for AAV and anti-infection measures. 7,8) As shown in Fig. 4, AAV frequently develops in aged people, and induces systemic vasculitis causing disorders in the kidneys and respiratory organ as two major vital organs.…”