Solubilized zona pellucida proteins and progesterone induce calcium influx and the acrosome reaction in capacitated dog spermatozoa

Brewis, Ian Andrew; Morton, I E; Moore, H. D. M.; England, G. C. W.

doi:10.1002/mrd.1114

Cited by 32 publications

(18 citation statements)

References 35 publications

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“…The latter may include a rise in progesterone concentrations and factors deriving from the maturing oocytes [10,40,41]. In our study despite the low sperm numbers detected in oviduct flushing, viable sperm were found both 2 days and 4 days after ovulation with a maximum proportion of 60% motile spermatozoa (group 2) similar to the in vitro findings of England and Pacey (1998) [42] and Pacey et al (2000) [43].…”

Section: Discussionsupporting

confidence: 88%

Distribution and viability of spermatozoa in the canine female genital tract during post-ovulatory oocyte maturation

et al. 2012

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BackgroundUnlike other domestic mammals, in which metaphase-II oocytes are ovulated, canine ovulation is characterized by the release of primary oocytes, which may take 12 to up to 36 hours. Further 60 hours are needed for maturation to secondary oocytes which then remain fertile for about 48 hours. Oestrus takes 7 to 10 days on average and may start as early as a week before ovulation. This together with the prolonged process of post-ovulatory oocyte maturation requires an according longevity of spermatozoa in the female genital tract in order to provide a population of fertile sperm when oocytes have matured to fertilizability. Therefore the distribution and viability of spermatozoa in the bitch genital tract was examined during post-ovulatory oocyte maturation.MethodsThirteen beagle bitches were inseminated on the day of sonographically verified ovulation with pooled semen of two beagle dogs containing one billion progressively motile spermatozoa. Ovariohysterectomy was performed two days later (group 1, n = 6) and four days later (group 2, n = 7). The oviduct and uterine horn of one side were flushed separately and the flushing’s were checked for the presence of gametes. The oviducts including the utero-tubal junction and the uterine horns, both the flushed and unflushed, were histologically examined for sperm distribution.ResultsThe total number of spermatozoa recovered by flushing was low and evaluation of viability was limited. Prophase-I oocytes were collected from oviduct flushing in group 1, whereas unfertilized metaphase-II oocytes were detected in group 2. From day 2 to day 4 after ovulation a significant decrease in the percentage of glands containing sperm (P<0.05) and a marked reduction of the mean sperm number in uterine horn glands were observed. A concomitant diminution of spermatozoa was indicated in the utero-tubal junction accompanied by a slight increase in sperm numbers in the mid oviduct.ConclusionsOocyte maturation to metaphase-II stage is accompanied by a continuous sperm detachment and elimination in the uterine horns. Entrance of spermatozoa into the caudal oviduct seems to be steadily controlled by the utero-tubal junction thus providing a selected sperm population to be shifted towards the site of fertilization when oocyte maturation is completed.

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Section: Discussionsupporting

confidence: 88%

Distribution and viability of spermatozoa in the canine female genital tract during post-ovulatory oocyte maturation

et al. 2012

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“…It can be argued that P facilitated IVF by promoting both hyperactivation and the acrosome reaction. Many studies report that this hormone, secreted by cumulus cells and contained in FF, induces AR in human (Meizel and Turner 1991;Baldi et al 1998), mouse (Roldan et al 1994), boar (Melendrez et al 1994), stallion (Meyers et al 1995;Cheng et al 1998), golden hamster (Llanos and Anabalón 1996), dog (Brewis et al 2001) and caprine (Somanath et al 2000) spermatozoa. Progesterone also affects human sperm capacitation (Foresta et al 1992;Uhler et al 1992;Luconi et al 1995) and increases the ability of mouse spermatozoa to respond to the zona pellucida (Roldan et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Leptin and leptin receptor are detectable in equine spermatozoa but are not involved in in vitro fertilisation

Consiglio

Corradetti

Perrini

et al. 2016

Reprod. Fertil. Dev.

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In human and swine, leptin (OB) has been identified in seminal plasma and leptin receptors (OB-R) on the cell surface of spermatozoa, indicating that spermatozoa are a target for OB. This hormone has also been detected in follicular fluid (FF) in women and mares, although its role requires further study. The aims of this study were to investigate the immunolocalisation and the expression of OB and OB-R in equine spermatozoa and to evaluate the involvement of OB in equine in vitro fertilisation (IVF). Since progesterone (P) and OB are both found in FF, the individual and combined effects of these two hormones were studied in equine IVF and compared with the results obtained from the use of FF for in vitro sperm preparation. For the first time, we were able to identify OB and OB-R mRNA and their corresponding proteins in equine spermatozoa. When spermatozoa were treated with OB, there was a decrease in the three motility parameters VSL, STR and LIN, commonly associated with hyperactivation, whilst the acrosome reaction rate increased (P<0.05). The fertilisation rate was 51% with FF, 46.15% with P, 43.64% with P+OB and 0% with OB alone. The percentage of eight-cell stage embryos was 18.7% with FF, 17.1% with P and 16.7% with OB+P. OB alone did not permit oocyte fertilisation, indicating that, in the horse, OB is involved in capacitation and hyperactivation but not in sperm penetration.

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“…1995), caprine (Somanath et al. 2000), canine (Brewis et al. 2001) and golden hamster (Meizel et al.…”

Section: Introductionmentioning

confidence: 99%

Effect of Exogenous Progesterone on Post‐thaw Capacitation and Acrosome Reaction of Bovine Spermatozoa

Lukoševičiūtė¹,

Žilinskas²,

Januškauskas³

2004

Reprod Domestic Animals

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The aim of this work was to study the effect of progesterone (P4) on capacitation and acrosome reaction (AR) of post-thaw bovine spermatozoa in vitro. Spermatozoa were incubated (0-180 min) in capacitation medium supplemented with 0, 0.1, 1.0 and 10.0 microg/ml of P4. At different time intervals aliquots were taken to determine sperm plasma membrane lipid destabilization, or capacitation (AR induced by lysophosphatidylcholine) in spermatozoa. The second experiment aimed to study the effects of P4, as potential inducer of AR in heparin-capacitated spermatozoa. The acrosomal status and viability of spermatozoa were evaluated under an epifluorescence microscope using Ethidium homodimer/peanut agglutinin fluorescein isothiocyanate staining method. Plasma membrane scrambling in spermatozoa was assessed by a flow cytometer, using merocyanine staining. The results show that P4 at the concentrations used had no negative effects on sperm viability. Progesterone significantly enhanced sperm capacitation (p < 0.001), but had no effect on plasma membrane lipid stability (p > 0.05) and did not significantly increase the AR of heparin-capacitated spermatozoa (p > 0.05). Progesterone displayed its effects in a dose-dependent manner with a maximum effect of 10 microg/ml P4 at 180 min of incubation. The results demonstrate that in cryopreserved bovine semen, P4 acts as capacitating, but not as an AR-inducing agent.

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Solubilized zona pellucida proteins and progesterone induce calcium influx and the acrosome reaction in capacitated dog spermatozoa

Cited by 32 publications

References 35 publications

Distribution and viability of spermatozoa in the canine female genital tract during post-ovulatory oocyte maturation

Distribution and viability of spermatozoa in the canine female genital tract during post-ovulatory oocyte maturation

Leptin and leptin receptor are detectable in equine spermatozoa but are not involved in in vitro fertilisation

Effect of Exogenous Progesterone on Post‐thaw Capacitation and Acrosome Reaction of Bovine Spermatozoa

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