Soluble adhesion molecule profile in normal pregnancy and pre-eclampsia

Chaiworapongsa, Tinnakorn; Romero, Roberto; Yoshimatsu, Jun; Espinoza, Jimmy; Kim, Y.M.; Park, K.; Kalache, Karim; Edwin, Samuel S.; Bujold, Emmanuel; Gomez, Ricardo

doi:10.1080/jmf.12.1.19.27

Cited by 109 publications

(35 citation statements)

References 17 publications

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“…We measured vascular cell adhesion molecule (VCAM-1), a widely accepted marker of endothelial dysfunction 34, 35 . As expected, in the presence of 1 ng/ml TNFα, VCAM-1 expression was induced 300-fold compared to cells with no TNFα (Fig.…”

Section: Resultsmentioning

confidence: 99%

Resveratrol inhibits release of soluble fms-like tyrosine kinase (sFlt-1) and soluble endoglin and improves vascular dysfunction – implications as a preeclampsia treatment

Hannan

Brownfoot

Cannon

et al. 2017

Sci Rep

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Preeclampsia is a disease of pregnancy associated with placental oxidative stress, inflammation and elevated release of anti-angiogenic factors sFlt-1 and soluble endoglin. These placental factors cause generalized maternal endothelial dysfunction. There are no treatments to halt disease progression; delivery is the only cure. Resveratrol modulates pathways involved in inflammation and oxidative stress and may offer a potential therapeutic for preeclampsia. Resveratrol reduced sFlt-1, sFlt-1 e15a and soluble endoglin secretion from primary trophoblasts and HUVECs and reduced mRNA expression of pro-inflammatory molecules NFκB, IL-6 and IL-1β in trophoblasts. IL-6, IL-1β and TNFα secretion were also significantly reduced. In HUVECs, resveratrol significantly increased mRNA of anti-oxidant enzymes HO-1, NQO1, GCLC and TXN but did not significantly alter HO-1 protein expression, whilst reducing HO-1 protein in trophoblast. Endothelial dysfunction was induced in HUVECs using TNFα, increasing expression of cell adhesion molecule VCAM1 and adhesion of peripheral blood mononuclear cells, both of which were increased further by resveratrol. In contrast, resveratrol significantly reduced TNFα-induced Endothelin-1 (a vasoconstrictor) and significantly increased the phosphorylation of endothelial nitric oxide synthase (eNOS). In summary, resveratrol decreases secretion of anti-angiogenic factors however its effects on the endothelium are mixed. Overall, it may have potential as a treatment for preeclampsia.

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Section: Resultsmentioning

confidence: 99%

Resveratrol inhibits release of soluble fms-like tyrosine kinase (sFlt-1) and soluble endoglin and improves vascular dysfunction – implications as a preeclampsia treatment

Hannan

Brownfoot

Cannon

et al. 2017

Sci Rep

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“…Previous reports indicated that cell adhesion has a critical role in NP (Konijnenberg et al 1997, Lyall et al 1999, Chaiworapongsa et al 2002, T cell adhesion to the extracellular matrix proteins, which was seen as a determinant of successful or failing pregnancy (Malgorzata & Gorski 1998). In women with RSA, the adhesion of trophoblasts to endothelial cells is inhibited.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients

Dong¹,

Zhang²,

Xia³

et al. 2014

Reproduction

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MicroRNAs (miRNAs) are non-coding RNA molecules of about 22 nucleotides that involved in post-transcriptional gene regulation. Evidence indicates that miRNAs play essential roles in endometriosis, pre-eclampsia, infertility and other reproductive system diseases. However, whether miRNAs are involved in recurrent spontaneous abortion (RSA) is unclear. In this work, we analysed the miRNA expression profiles in six pairs of villus or decidua from RSA patients and normal pregnancy (NP) women using a human miRNA microarray. Some of the chip results were confirmed by RT-qPCR. In the villi of RSA patients, expression of hsa-miR-184, hsa-miR-187 and hsa-miR-125b-2 was significantly higher, while expression of hsa-miR-520f, hsa-miR-3175 and hsa-miR-4672 was significantly lower, comparing with those of NP control. As well, a total of five miRNAs (hsa-miR-517c, hsa-miR-519a-1, hsa-miR-522, hsa-miR-520h and hsa-miR-184) were upregulated in the decidua of RSA patients. The target genes of these differentially expressed miRNAs were predicted by miRWalk, and we speculate a network of miRNA regulating RSA by target genes function on adhesion, apoptosis and angiogenesis. Our study may help clarify the molecular mechanisms which are involved in the progression of RSA, and provide a reference for future research.

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“…Preeclampsia is considered to be characterized by endothelial cell activation/dysfunction [17-24], and this condition is associated with a significant increase in the maternal circulating concentrations of s E-selectin [57,58,60,158,159] and sVCAM-1 [57,60,158,160,161], which are markers of endothelial cell activation/dysfunction [136,162]. However, there is no increase in the concentration of sICAM-1 and sPECAM-1 [57,60,163,164], indicating that there are some unique features of endothelial cell activation/dysfunction in preeclampsia. When assessing the profile of adhesion molecules, we have found that patients with acute pyelonephritis have an increase in sICAM-1, sE-selectin, and sVCAM-1 [60], suggesting that there are subtle differences in the adhesion molecule profile in patients with pyelonephritis and preeclampsia, both of which are characterized by intravascular inflammation [12,157].…”

Section: Discussionmentioning

confidence: 99%

“…However, there is no increase in the concentration of sICAM-1 and sPECAM-1 [57,60,163,164], indicating that there are some unique features of endothelial cell activation/dysfunction in preeclampsia. When assessing the profile of adhesion molecules, we have found that patients with acute pyelonephritis have an increase in sICAM-1, sE-selectin, and sVCAM-1 [60], suggesting that there are subtle differences in the adhesion molecule profile in patients with pyelonephritis and preeclampsia, both of which are characterized by intravascular inflammation [12,157]. …”

Section: Discussionmentioning

confidence: 99%

Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes

Adekola

Romero

Chaemsaithong

et al. 2014

The Journal of Maternal-Fetal & Neonatal Medicine

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Objective: Endocan, a dermatan sulphate proteoglycan produced by endothelial cells, is considered a biomarker for endothelial cell activation/dysfunction. Preeclampsia is characterized by systemic vascular inflammation, and endothelial cell activation/dysfunction. Therefore, the objectives of this study were to determine whether: (1) plasma endocan concentrations in preeclampsia differ from those in uncomplicated pregnancies; (2) changes in plasma endocan concentration relate to the severity of preeclampsia, and whether these changes are specific or observed in other obstetrical syndromes such as small-for-gestational age (SGA), fetal death (FD), preterm labor (PTL) or preterm prelabor rupture of membranes (PROM); (3) a correlation exists between plasma concentration of endocan and angiogenic (placental growth factor or PlGF)/anti-angiogenic factors (soluble vascular endothelial growth factor receptor or sVEGFR-1, and soluble endoglin or sEng) among pregnancies complicated by preeclampsia; and (4) plasma endocan concentrations in patients with preeclampsia and acute pyelonephritis (both conditions in which there is endothelial cell activation) differ. Method: This cross-sectional study included the following groups: (1) uncomplicated pregnancy (n = 130); (2) preeclampsia (n = 102); (3) pregnant women without preeclampsia who delivered an SGA neonate (n = 51); (4) FD (n = 49); (5) acute pyelonephritis (AP; n = 35); (6) spontaneous PTL (n = 75); and (7) preterm PROM (n = 64). Plasma endocan concentrations were determined in all groups, and PIGF, sEng and VEGFR-1 plasma concentrations were measured by ELISA in the preeclampsia group. Results: (1) Women with preeclampsia had a significantly higher median plasma endocan concentration than those with uncomplicated pregnancies (p = 0.004); (2) among women with preeclampsia, the median plasma endocan concentration did not differ significantly according to disease severity (p = 0.1), abnormal uterine artery Doppler velocimetry (p = 0.7) or whether diagnosis was made before or after 34 weeks gestational age (p = 0.3); (3) plasma endocan concentration in women with preeclampsia correlated positively with plasma anti-angiogenic factor concentrations [sVEGFR-1: Spearman rho 0.34, p = 0.001 and sEng: Spearman rho 0.30, p = 0.003]; (4) pregnancies complicated by acute pyelonephritis with bacteremia had a lower median plasma endocan concentration than pregnancies complicated by acute pyelonephritis without bacteremia (p = 0.004), as well as uncomplicated pregnancies (p = 0.001); and (5) there was no significant difference in the median plasma endocan concentration between uncomplicated pregnancies and those complicated by FD, delivery of an SGA neonate, PTL or preterm PROM (other members of the “great obstetrical syndromes”; each p > 0.05). Conclusion: Median maternal plasma endocan concentrations were higher preeclampsia and lower in acute pyelonephritis with bacteremia than in uncomplicated pregnancy. No significant difference was observed in the median plasma endocan...

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Soluble adhesion molecule profile in normal pregnancy and pre-eclampsia

Cited by 109 publications

References 17 publications

Resveratrol inhibits release of soluble fms-like tyrosine kinase (sFlt-1) and soluble endoglin and improves vascular dysfunction – implications as a preeclampsia treatment

Resveratrol inhibits release of soluble fms-like tyrosine kinase (sFlt-1) and soluble endoglin and improves vascular dysfunction – implications as a preeclampsia treatment

Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients

Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes

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